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Int Sch Res Notices. 2014 Oct 28;2014:976264. doi: 10.1155/2014/976264. eCollection 2014.

Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC.

International scholarly research notices

Sharada L Deore, Payal S Jaju, Bhushan A Baviskar

Affiliations

  1. Government College of Pharmacy, Kathora Naka, Amravati 444604, India.

PMID: 27382629 PMCID: PMC4897258 DOI: 10.1155/2014/976264

Abstract

A new simple, rapid, selective and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous estimation of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup. The retention factors of vasicine, glycyrrhizin, eugenol, and cineole are 0.53, 0.44, 0.75, and 0.77, respectively. Chromatography was performed on 60F254 percolated TLC plate using n-hexane : ethyl acetate : glacial acetic acid (8.5 : 1.0 : 0.5 v/v/v). Methods are validated according to ICH guidelines and can be adopted for the routine analysis of vasicine, glycyrrhizin, eugenol and cineole in herbal cough syrup.

References

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