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Mattioli TM, Alanis LR, Sapelli Sda S, et al. Effects of Benzodiazepines on Acinar and Myoepithelial Cells. Front Pharmacol. 2016;7:173doi: 10.3389/fphar.2016.00173.
Mattioli, T. M., Alanis, L. R., Sapelli, S. d. a. . S., de Lima, A. A., de Noronha, L., Rosa, E. A., Althobaiti, Y. S., Almalki, A. H., Sari, Y., Ignacio, S. A., Johann, A. C., & Gregio, A. M. (2016). Effects of Benzodiazepines on Acinar and Myoepithelial Cells. Frontiers in pharmacology, 7173. https://doi.org/10.3389/fphar.2016.00173
Mattioli, Tatiana M F, et al. "Effects of Benzodiazepines on Acinar and Myoepithelial Cells." Frontiers in pharmacology vol. 7 (2016): 173. doi: https://doi.org/10.3389/fphar.2016.00173
Mattioli TM, Alanis LR, Sapelli Sda S, de Lima AA, de Noronha L, Rosa EA, Althobaiti YS, Almalki AH, Sari Y, Ignacio SA, Johann AC, Gregio AM. Effects of Benzodiazepines on Acinar and Myoepithelial Cells. Front Pharmacol. 2016 Jun 24;7:173. doi: 10.3389/fphar.2016.00173. eCollection 2016. PMID: 27445812; PMCID: PMC4919344.
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Front Pharmacol. 2016 Jun 24;7:173. doi: 10.3389/fphar.2016.00173. eCollection 2016.

Effects of Benzodiazepines on Acinar and Myoepithelial Cells.

Frontiers in pharmacology

Tatiana M F Mattioli, Luciana R A Alanis, Silvana da Silva Sapelli, Antonio A S de Lima, Lucia de Noronha, Edvaldo A R Rosa, Yusuf S Althobaiti, Atiah H Almalki, Youssef Sari, Sergio A Ignacio, Aline C B R Johann, Ana M T Gregio

Affiliations

  1. Pharmacology and Experimental Pathology, School of Dentistry, Pontifical Catholic University of Paraná Curitiba, Brazil.
  2. Program of Post-Graduation, School of Dentistry, Health and Bioscience School, Pontifical Catholic University of Paraná Curitiba, Brazil.
  3. Department of Oral Pathology, School of Dentistry, Federal University of Paraná Curitiba, Brazil.
  4. Program of Post-Graduation, Department of Pathology, School of Medicine, Pontifical Catholic University of Paraná Curitiba, Brazil.
  5. Department of Pharmacology, College of Pharmacy and Pharmaceutical, The University of Toledo Toledo, OH, US.
  6. Department of Pharmacology, College of Pharmacy and Pharmaceutical, The University of ToledoToledo, OH, US; Program of Post-Graduation, Department of Pharmacology, School of Dentistry, Health and Bioscience School, Pontifical Catholic University of ParanáCuritiba, Brazil.

PMID: 27445812 PMCID: PMC4919344 DOI: 10.3389/fphar.2016.00173

Abstract

BACKGROUND: Benzodiazepines (BZDs), the most commonly prescribed psychotropic drugs with anxiolytic action, may cause hyposalivation. It has been previously shown that BZDs can cause hypertrophy and decrease the acini cell number. In this study, we investigated the effects of BZDs and pilocarpine on rat parotid glands, specifically on acinar, ductal, and myoepithelial cells.

METHODS: Ninety male Wistar rats were divided into nine groups. Control groups received a saline solution for 30 days (C30) and 60 days (C60), and pilocarpine (PILO) for 60 days. Experimental groups received lorazepam (L30) and midazolam (M30) for 30 days. Another group (LS60 or MS60) received lorazepam or midazolam for 30 days, respectively, and saline for additional 30 days. Finally, other groups (LP60 or MP60) received either lorazepam or midazolam for 30 days, respectively, and pilocarpine for additional 30 days. The expression of calponin in myoepithelial cells and the proliferating cell nuclear antigen (PCNA) in acinar and ductal cells were evaluated.

RESULTS: Animals treated with lorazepam showed an increase in the number of positive staining cells for calponin as compared to control animals (p < 0.05). Midazolam administered with pilocarpine (MP60) induced an increase in the proliferation of acinar and ductal cells and a decrease in the positive staining cells for calponin as compared to midazolam administered with saline (MS60).

CONCLUSION: We found that myoepithelial cells might be more sensitive to the effects of BZD than acinar and ductal cells in rat parotid glands.

Keywords: GABAA receptor; benzodiazepines; dry mouth; immunohistochemistry; salivary glands

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