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Bronnec V, Turoňová H, Bouju A, et al. Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions. Front Microbiol. 2016;7:1002doi: 10.3389/fmicb.2016.01002.
Bronnec, V., Turoňová, H., Bouju, A., Cruveiller, S., Rodrigues, R., Demnerova, K., Tresse, O., Haddad, N., & Zagorec, M. (2016). Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions. Frontiers in microbiology, 71002. https://doi.org/10.3389/fmicb.2016.01002
Bronnec, Vicky, et al. "Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions." Frontiers in microbiology vol. 7 (2016): 1002. doi: https://doi.org/10.3389/fmicb.2016.01002
Bronnec V, Turoňová H, Bouju A, Cruveiller S, Rodrigues R, Demnerova K, Tresse O, Haddad N, Zagorec M. Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions. Front Microbiol. 2016 Jun 30;7:1002. doi: 10.3389/fmicb.2016.01002. eCollection 2016. PMID: 27446042; PMCID: PMC4927563.
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Front Microbiol. 2016 Jun 30;7:1002. doi: 10.3389/fmicb.2016.01002. eCollection 2016.

Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions.

Frontiers in microbiology

Vicky Bronnec, Hana Turoňová, Agnès Bouju, Stéphane Cruveiller, Ramila Rodrigues, Katerina Demnerova, Odile Tresse, Nabila Haddad, Monique Zagorec

Affiliations

  1. UMR 1014 SECALIM, Oniris Nantes, France.
  2. Institute of Chemical Technology, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology Prague, Czech Republic.
  3. CNRS-UMR 8030 and Commissariat à l'Energie Atomique et aux Energies Alternatives CEA/DRF/IG/Genoscope LABGeM Evry, France.

PMID: 27446042 PMCID: PMC4927563 DOI: 10.3389/fmicb.2016.01002

Abstract

Campylobacter jejuni is the leading cause of bacterial enteritis in Europe. Human campylobacteriosis cases are frequently associated to the consumption of contaminated poultry meat. To survive under environmental conditions encountered along the food chain, i.e., from poultry digestive tract its natural reservoir to the consumer's plate, this pathogen has developed adaptation mechanisms. Among those, biofilm lifestyle has been suggested as a strategy to survive in the food environment and under atmospheric conditions. Recently, the clinical isolate C. jejuni Bf has been shown to survive and grow under aerobic conditions, a property that may help this strain to better survive along the food chain. The aim of this study was to evaluate the adhesion capacity of C. jejuni Bf and its ability to develop a biofilm. C. jejuni Bf can adhere to abiotic surfaces and to human epithelial cells, and can develop biofilm under both microaerobiosis and aerobiosis. These two conditions have no influence on this strain, unlike results obtained with the reference strain C. jejuni 81-176, which harbors only planktonic cells under aerobic conditions. Compared to 81-176, the biofilm of C. jejuni Bf is more homogenous and cell motility at the bottom of biofilm was not modified whatever the atmosphere used. C. jejuni Bf whole genome sequence did not reveal any gene unique to this strain, suggesting that its unusual property does not result from acquisition of new genetic material. Nevertheless some genetic particularities seem to be shared only between Bf and few others strains. Among the main features of C. jejuni Bf genome we noticed (i) a complete type VI secretion system important in pathogenicity and environmental adaptation; (ii) a mutation in the oorD gene involved in oxygen metabolism; and (iii) the presence of an uncommon insertion of a 72 amino acid coding sequence upstream from dnaK, which is involved in stress resistance. Therefore, the atypical behavior of this strain under aerobic atmosphere may result from the combination of insertions and mutations. In addition, the comparison of mRNA transcript levels of several genes targeted through genome analysis suggests the modification of regulatory processes in this strain.

Keywords: biofilm; confocal microscopy; food borne pathogen; genome sequence; oxidative stress

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