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Abbandonato G, Storti B, Signore G, et al. Quantitative optical lock-in detection for quantitative imaging of switchable and non-switchable components. Microsc Res Tech. 2016;79(10):929-937doi: 10.1002/jemt.22724.
Abbandonato, G., Storti, B., Signore, G., Beltram, F., & Bizzarri, R. (2016). Quantitative optical lock-in detection for quantitative imaging of switchable and non-switchable components. Microscopy research and technique, 79(10), 929-937. https://doi.org/10.1002/jemt.22724
Abbandonato, Gerardo, et al. "Quantitative optical lock-in detection for quantitative imaging of switchable and non-switchable components." Microscopy research and technique vol. 79,10 (2016): 929-937. doi: https://doi.org/10.1002/jemt.22724
Abbandonato G, Storti B, Signore G, Beltram F, Bizzarri R. Quantitative optical lock-in detection for quantitative imaging of switchable and non-switchable components. Microsc Res Tech. 2016 Oct;79(10):929-937. doi: 10.1002/jemt.22724. Epub 2016 Jul 22. PMID: 27447845.
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Microsc Res Tech. 2016 Oct;79(10):929-937. doi: 10.1002/jemt.22724. Epub 2016 Jul 22.

Quantitative optical lock-in detection for quantitative imaging of switchable and non-switchable components.

Microscopy research and technique

Gerardo Abbandonato, Barbara Storti, Giovanni Signore, Fabio Beltram, Ranieri Bizzarri

Affiliations

  1. NEST, Scuola Normale Superiore and Istituto Nanoscienze, piazza San Silvestro 12, I-56127, Italy.
  2. Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, piazza San Silvestro 12, I-56127, Italy.
  3. NEST, Scuola Normale Superiore and Istituto Nanoscienze, piazza San Silvestro 12, I-56127, Italy. [email protected].

PMID: 27447845 DOI: 10.1002/jemt.22724

Abstract

Reversible photoswitching has been proposed as a way to identify molecules that are present in small numbers over a large, non-switching, background. This approach, called optical-lock-in-detection (OLID) requires the deterministic control of the fluorescence of a photochromic emitter through optical modulation between a bright (on) and a dark state (off). OLID yields a high-contrast map where the switching molecules are pinpointed, but the fractional intensities of the emitters are not returned. The present work presents a modified OLID approach (quantitative OLID or qOLID) that yields quantitative information of the switching (f

© 2016 Wiley Periodicals, Inc.

Keywords: TRPV1; optical lock-in detection; photochromic FRET; photochromism; reversibly switchable fluorescent proteins

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