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Mahey S, Kumar R, Arora R, et al. Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines. Springerplus. 2016;5(1):930doi: 10.1186/s40064-016-2405-0.
Mahey, S., Kumar, R., Arora, R., Mahajan, J., Arora, S., Bhardwaj, R., & Thukral, A. K. (2016). Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines. SpringerPlus, 5(1), 930. https://doi.org/10.1186/s40064-016-2405-0
Mahey, Sonia, et al. "Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines." SpringerPlus vol. 5,1 (2016): 930. doi: https://doi.org/10.1186/s40064-016-2405-0
Mahey S, Kumar R, Arora R, Mahajan J, Arora S, Bhardwaj R, Thukral AK. Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines. Springerplus. 2016 Jun 30;5(1):930. doi: 10.1186/s40064-016-2405-0. eCollection 2016. PMID: 27386374; PMCID: PMC4929113.
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Springerplus. 2016 Jun 30;5(1):930. doi: 10.1186/s40064-016-2405-0. eCollection 2016.

Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines.

SpringerPlus

Sonia Mahey, Rakesh Kumar, Rohit Arora, Jyoti Mahajan, Saroj Arora, Renu Bhardwaj, Ashwani Kumar Thukral

Affiliations

  1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
  2. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India ; Department of Botany, DAV University, Jalandhar, Punjab 144012 India.

PMID: 27386374 PMCID: PMC4929113 DOI: 10.1186/s40064-016-2405-0

Abstract

The present study investigates the anti-proliferative and apoptosis inducing mechanism of CoCl2·6H2O in PC-3 cancer cell line. Preliminary, three different forms of cobalt i.e., cobaltous (CoCl2·6H2O), macro-Co(II,III) oxide and nano-Co(II,III) oxide were screened for antiproliferative activity in PC-3 cell line. The CoCl2·6H2O being the most effective antiproliferative agent, hence it was further tested against lung (A549), prostrate (PC-3) and brain (IMR-32) cell lines. Human embryonic kidney cell line (293T) was used as a normal cell line to compare the toxicity of CoCl2·6H2O. The CoCl2·6H2O induced morphological and anatomical changes in PC-3 cancer cell which were studied using light, confocal and scanning electron microscopy. The lactate dehydrogenase was estimated which showed mild necrotic mode of cell death. The Annexin/PI staining confirmed the apoptotic mode of cell death in PC-3 cells. Further, production of reaction of reactive oxygen species and changes in mitochondrial membrane potential was also assessed spectrofluorimetrically. The cell cycle arrest was also investigated using flow cytometery. Finally, the caspase activity was estimated in CoCl2·6H2O treated PC-3 cancer cell line. Interestingly, it was found that CoCl2·6H2O induces more cell death in cancerous cells as compared to normal non-cancerous cells. These findings presented CoCl2·6H2O as potential antiproliferative agent.

Keywords: Antiproliferative; Apoptosis; CoCl2·6H2O; MTT

References

  1. Arterioscler Thromb Vasc Biol. 1999 Sep;19(9):2154-61 - PubMed
  2. Cancer Res. 2000 Jan 1;60(1):38-41 - PubMed
  3. Steroids. 2002 Dec;67(13-14):1029-40 - PubMed
  4. Am J Respir Cell Mol Biol. 2005 May;32(5):395-403 - PubMed
  5. Br J Sports Med. 2005 Nov;39(11):872-3 - PubMed
  6. Toxicol Pathol. 2007 Jun;35(4):495-516 - PubMed
  7. Apoptosis. 2007 Oct;12(10):1911-26 - PubMed
  8. Int J Oncol. 2007 Oct;31(4):923-9 - PubMed
  9. Food Chem Toxicol. 2008 Aug;46(8):2922-7 - PubMed
  10. Acta Pharmacol Sin. 2008 Aug;29(8):881-90 - PubMed
  11. Dalton Trans. 2009 Oct 7;(37):7588-98 - PubMed
  12. Mol Vis. 2009 Dec 16;15:2789-95 - PubMed
  13. Expert Opin Pharmacother. 2010 Feb;11(2):249-56 - PubMed
  14. Mol Pharm. 2011 Dec 5;8(6):2368-78 - PubMed
  15. Anat Rec (Hoboken). 2012 Mar;295(3):417-22 - PubMed
  16. Exp Toxicol Pathol. 2013 Mar;65(3):341-9 - PubMed
  17. Cancer Cell Int. 2012 May 15;12(1):18 - PubMed
  18. Future Med Chem. 2012 Jun;4(10):1257-72 - PubMed
  19. Clin Chem Lab Med. 2013 Jan;51(1):139-55 - PubMed
  20. Curr Pharm Des. 2014;20(2):223-44 - PubMed
  21. J Vet Sci. 2013;14(3):281-9 - PubMed
  22. BMC Complement Altern Med. 2013 Sep 10;13:223 - PubMed
  23. Dis Model Mech. 2013 Nov;6(6):1507-14 - PubMed
  24. Eur J Clin Nutr. 2014 Jan;68(1):2-7 - PubMed
  25. PLoS One. 2014 Mar 18;9(3):e90968 - PubMed
  26. Metallomics. 2014 May;6(5):1014-22 - PubMed
  27. Sci Rep. 2014 Apr 15;4:4698 - PubMed
  28. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Nov 11;132:663-71 - PubMed
  29. Eur J Med Chem. 2014 Jul 23;82:574-83 - PubMed
  30. Atherosclerosis. 2014 Aug;235(2):649-58 - PubMed
  31. Sci Rep. 2014 Aug 05;4:5955 - PubMed
  32. Chemistry. 2014 Oct 6;20(41):13103-7 - PubMed
  33. J Inorg Biochem. 2015 Jan;142:1-7 - PubMed
  34. Biometals. 2015 Feb;28(1):175-85 - PubMed
  35. Cancer Lett. 2015 Mar 1;358(1):8-16 - PubMed
  36. Cell Death Dis. 2015 Jan 15;6:e1590 - PubMed
  37. Mol Cancer. 2015 Jan 21;14:6 - PubMed
  38. Toxicol In Vitro. 2015 Apr;29(3):551-7 - PubMed
  39. Philos Trans A Math Phys Eng Sci. 2015 Mar 13;373(2037):null - PubMed
  40. Chem Soc Rev. 2015 Apr 21;44(8):2529-42 - PubMed
  41. Sci Rep. 2015 Mar 17;5:9157 - PubMed
  42. Exp Ther Med. 2015 Apr;9(4):1465-1469 - PubMed
  43. Nat Commun. 2015 Mar 20;6:6582 - PubMed
  44. Sci Rep. 2017 Jun 05;7:46793 - PubMed

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