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Johnson KH, Webb CH, Schmeling DO, et al. Epstein-Barr virus dynamics in asymptomatic immunocompetent adults: an intensive 6-month study. Clin Transl Immunology. 2016;5(5):e81doi: 10.1038/cti.2016.28.
Johnson, K. H., Webb, C. H., Schmeling, D. O., Brundage, R. C., & Balfour, H. H. (2016). Epstein-Barr virus dynamics in asymptomatic immunocompetent adults: an intensive 6-month study. Clinical & translational immunology, 5(5), e81. https://doi.org/10.1038/cti.2016.28
Johnson, Kristin H, et al. "Epstein-Barr virus dynamics in asymptomatic immunocompetent adults: an intensive 6-month study." Clinical & translational immunology vol. 5,5 (2016): e81. doi: https://doi.org/10.1038/cti.2016.28
Johnson KH, Webb CH, Schmeling DO, Brundage RC, Balfour HH. Epstein-Barr virus dynamics in asymptomatic immunocompetent adults: an intensive 6-month study. Clin Transl Immunology. 2016 May 13;5(5):e81. doi: 10.1038/cti.2016.28. eCollection 2016 May. PMID: 27350880; PMCID: PMC4910122.
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Clin Transl Immunology. 2016 May 13;5(5):e81. doi: 10.1038/cti.2016.28. eCollection 2016 May.

Epstein-Barr virus dynamics in asymptomatic immunocompetent adults: an intensive 6-month study.

Clinical & translational immunology

Kristin H Johnson, Chiu-Ho Webb, David O Schmeling, Richard C Brundage, Henry H Balfour

Affiliations

  1. Department of Laboratory Medicine and Pathology, University of Minnesota Medical School , Minneapolis, MN, USA.
  2. Department of Experimental and Clinical Pharmacology, University of Minnesota Medical School , Minneapolis, MN, USA.
  3. Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN, USA; Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.

PMID: 27350880 PMCID: PMC4910122 DOI: 10.1038/cti.2016.28

Abstract

Characterizing Epstein-Barr virus (EBV) dynamics in asymptomatic immunocompetent persons provides a baseline for defining quantitative thresholds associated with EBV disease. Studying latent membrane protein (LMP)-1 sequence variation over time could establish the rates of reactivation and superinfection, and also trace transmission. Twelve asymptomatic adult subjects were evaluated prospectively nine times over 6 months. EBV serum antibodies were measured by enzyme immunoassay. EBV DNA in oral and whole-blood samples was quantitated by real-time (TaqMan) PCR and analyzed for LMP-1 sequence variability. All 11 antibody positive subjects had EBV DNA detected in their oral compartment at least once during the 6-month study. The quantities ranged from 1.70 to 4.91 log10 copies EBV per ml of oral cell pellet. One subject was continuously viremic for 79 days. Overall, EBV DNA was detected in 63 (24%) of 260 samples from 11 antibody-positive subjects and in 0/27 samples from an antibody-negative subject. The quantities in positive samples ranged from 1.7 to 4.9 log10 copies EBV per ml. EBV LMP-1 gene sequence variations in subjects were constant over time regardless of the compartment sampled. Subjects 18-30 years old had EBV DNA detected more frequently than subjects >30 years old (38/108 positive samples versus 25/152; P<0.001). In conclusion, EBV DNA shedding is common in asymptomatic adults. The younger adults shed more frequently, which may reflect a shorter time from their primary EBV infection to sampling. The LMP-1 sequence analysis method employed here could be used to trace person-to-person transmission because patterns remained almost identical over time.

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