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Goto-Inoue N, Kashiwagi A, Kashiwagi K, et al. Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging. Biol Open. 2016;5(9):1252-9doi: 10.1242/bio.019646.
Goto-Inoue, N., Kashiwagi, A., Kashiwagi, K., & Mori, T. (2016). Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging. Biology open, 5(9), 1252-9. https://doi.org/10.1242/bio.019646
Goto-Inoue, Naoko, et al. "Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging." Biology open vol. 5,9 (2016): 1252-9. doi: https://doi.org/10.1242/bio.019646
Goto-Inoue N, Kashiwagi A, Kashiwagi K, Mori T. Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging. Biol Open. 2016 Sep 15;5(9):1252-9. doi: 10.1242/bio.019646. PMID: 27422901; PMCID: PMC5051643.
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Biol Open. 2016 Sep 15;5(9):1252-9. doi: 10.1242/bio.019646.

Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging.

Biology open

Naoko Goto-Inoue, Akihiko Kashiwagi, Keiko Kashiwagi, Tsukasa Mori

Affiliations

  1. Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan [email protected].
  2. Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
  3. Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan.

PMID: 27422901 PMCID: PMC5051643 DOI: 10.1242/bio.019646

Abstract

In developmental and cell biology it is crucial to evaluate the dynamic profiles of metabolites. An emerging frog model system using Xenopus tropicalis, whose genome sequence and inbred strains are available, is now ready for metabolomics investigation in amphibians. In this study we applied matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) analysis to identify and visualize metabolomic molecular markers in tadpoles of Xenopus tropicalis We detected tissue-specific peaks and visualized their distribution in tissues, and distinguished 19 tissues and their specific peaks. We identified, for the first time, some of their molecular localizations via tandem mass spectrometric analysis: hydrocortisone in artery, L-DOPA in rhombencephalon, taurine in eye, corticosterone in gill, heme in heart, inosine monophosphate and carnosine in muscle, dopamine in nerves, and phosphatidylethanolamine (16:0/20:4) in pharynx. This is the first MALDI-MSI study of X. tropicalis tadpoles, as in small tadpoles it is hard to distinguish and dissect the various organs. Furthermore, until now there has been no data about the metabolomic profile of each organ. Our results suggest that MALDI-MSI is potentially a powerful tool for examining the dynamics of metabolomics in metamorphosis as well as conformational changes due to metabolic changes.

© 2016. Published by The Company of Biologists Ltd.

Keywords: Imaging; Metabolites; Molecular marker; Tadpoles; Xenopus tropicalis

Conflict of interest statement

The authors declare no competing or financial interests.

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