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Kotlo K, Samarel AM, Chen HY, et al. Global comparison of phosphoproteins in human and rodent hearts: implications for translational studies of myosin light chain and troponin phosphorylations. Springerplus. 2016;5(1):808doi: 10.1186/s40064-016-2469-x.
Kotlo, K., Samarel, A. M., Chen, H. Y., Aldstadt, J., & Danziger, R. S. (2016). Global comparison of phosphoproteins in human and rodent hearts: implications for translational studies of myosin light chain and troponin phosphorylations. SpringerPlus, 5(1), 808. https://doi.org/10.1186/s40064-016-2469-x
Kotlo, K, et al. "Global comparison of phosphoproteins in human and rodent hearts: implications for translational studies of myosin light chain and troponin phosphorylations." SpringerPlus vol. 5,1 (2016): 808. doi: https://doi.org/10.1186/s40064-016-2469-x
Kotlo K, Samarel AM, Chen HY, Aldstadt J, Danziger RS. Global comparison of phosphoproteins in human and rodent hearts: implications for translational studies of myosin light chain and troponin phosphorylations. Springerplus. 2016 Jun 21;5(1):808. doi: 10.1186/s40064-016-2469-x. eCollection 2016. PMID: 27390648; PMCID: PMC4916117.
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Springerplus. 2016 Jun 21;5(1):808. doi: 10.1186/s40064-016-2469-x. eCollection 2016.

Global comparison of phosphoproteins in human and rodent hearts: implications for translational studies of myosin light chain and troponin phosphorylations.

SpringerPlus

K Kotlo, A M Samarel, H Y Chen, J Aldstadt, R S Danziger

Affiliations

  1. Division of Cardiology, University of Illinois at Chicago, 840 S. Wood St, Chicago, IL 60612 USA.
  2. The Cardiovascular Institute and the Department of Medicine, Loyola University Chicago Stritch School of Medicine, Building 110, Rm 5222, 2160 South First Avenue, Maywood, IL 60153 USA.
  3. Division of Epidemiology and Biostatistics, University of Illinois at Chicago, Chicago, IL 60612 USA.
  4. Department of Geography, University of Buffalo, 105 Wilkeson Quad, Buffalo, NY 14261 USA.
  5. Division of Cardiology, University of Illinois at Chicago, 840 S. Wood St, Chicago, IL 60612 USA ; Jesse Brown Veterans Administration, 820 S. Damen, Chicago, IL 60612 USA.

PMID: 27390648 PMCID: PMC4916117 DOI: 10.1186/s40064-016-2469-x

Abstract

Cardiac remodeling and failure are regulated by a myriad of cardiac protein phosphorylations. In the present study, cardiac phosphoprotein patterns were examined in rodent and human hearts Left ventricular tissue samples were obtained from human systolic failing (n = 5) and control (n = 5) hearts and from two rat models of hypertensive heart failure, i.e., spontaneously hypertensive heart failure and Dahl salt-sensitive rats and corresponding controls. Phosphoproteins were separated by 2D-DIGE with Cydye staining, phosphoprotein patterns were analyzed using pixel intensity in rectified images. Specific phosphoproteins which were different in human versus rodent hearts were identified by MALDI-TOF/TOF Mass Spectrometry. Targeted pair-wise analyses showed differences (p < 0.05) in 26 % of the pixels, which included pixels containing phosphorylated troponin T, myosin light chain, peroxiredoxin, and haptoglobin. These results show differences in rodent versus human cardiac remodeling which will influence the translation rodent studies to humans in this area.

References

  1. J Mol Cell Cardiol. 2013 Jul;60:116-20 - PubMed
  2. J Gen Physiol. 2005 Mar;125(3):257-71 - PubMed
  3. Br J Pharmacol. 2007 Jan;150(2):130-5 - PubMed
  4. Circ Res. 2004 Oct 1;95(7):717-25 - PubMed
  5. Front Biosci. 2008 May 01;13:5988-6005 - PubMed
  6. Adv Exp Med Biol. 2003;538:3-15 - PubMed
  7. Cardiovasc Res. 2003 Aug 1;59(2):400-11 - PubMed
  8. J Hypertens. 2008 Feb;26(2):334-44 - PubMed
  9. Mol Pharmacol. 2006 Dec;70(6):1992-2003 - PubMed
  10. FASEB J. 2007 Jun;21(8):1884-92 - PubMed
  11. Ann N Y Acad Sci. 1998 Sep 16;853:240-50 - PubMed
  12. Mol Cell Biochem. 2005 Jan;269(1-2):49-57 - PubMed
  13. Cardiovasc Res. 2008 Aug 1;79(3):464-71 - PubMed
  14. Circulation. 2000 Oct 31;102(18):2269-75 - PubMed
  15. J Clin Invest. 1990 May;85(5):1698-702 - PubMed
  16. Kidney Int. 1999 Jan;55(1):29-62 - PubMed
  17. Cardiovasc Res. 2002 Jan;53(1):131-7 - PubMed
  18. Trends Pharmacol Sci. 2008 Apr;29(4):175-80 - PubMed
  19. Circ Res. 2007 Oct 12;101(8):819-29 - PubMed
  20. J Thorac Cardiovasc Surg. 2007 Jan;133(1):37-43 - PubMed
  21. J Mol Cell Cardiol. 1992 Apr;24(4):387-96 - PubMed
  22. J Biol Chem. 2003 Sep 12;278(37):35135-44 - PubMed
  23. J Mol Cell Cardiol. 2006 Sep;41(3):441-50 - PubMed
  24. J Biol Chem. 2005 May 27;280(21):20814-23 - PubMed
  25. Circulation. 2006 Jul 25;114(4):298-308 - PubMed
  26. Circ Res. 2007 Jul 20;101(2):195-204 - PubMed
  27. JAMA. 2006 Oct 11;296(14):1731-2 - PubMed
  28. Pharmacol Res. 2007 Jun;55(6):511-22 - PubMed
  29. Circulation. 1995 Oct 15;92(8):2041-3 - PubMed
  30. Cardiovasc Res. 2001 Oct;52(1):95-102 - PubMed
  31. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1655-60 - PubMed
  32. Nat Med. 2005 Apr;11(4):409-17 - PubMed
  33. EMBO J. 2000 Jun 1;19(11):2537-48 - PubMed
  34. Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2373-81 - PubMed
  35. Circ Res. 2005 Jan 7;96(1):54-63 - PubMed
  36. Circulation. 1995 Sep 15;92 (6):1612-8 - PubMed
  37. Methods Enzymol. 2011;496:435-63 - PubMed
  38. Physiol Genomics. 2006 Oct 11;27(2):156-70 - PubMed
  39. Cell. 2002 Aug 23;110(4):479-88 - PubMed
  40. J Biol Chem. 2002 Jun 21;277(25):22896-901 - PubMed
  41. Mol Cell Biol. 2004 Oct;24(19):8374-85 - PubMed
  42. Mol Cell Biol. 2006 May;26(10):3875-88 - PubMed
  43. Cell. 2005 Oct 7;123(1):25-35 - PubMed
  44. Am J Physiol Heart Circ Physiol. 2007 Jun;292(6):H2782-90 - PubMed
  45. J Clin Invest. 2003 Mar;111(5):617-25 - PubMed
  46. J Am Coll Cardiol. 2008 Apr 15;51(15):1490-7 - PubMed
  47. Ann N Y Acad Sci. 2004 May;1015:39-52 - PubMed
  48. Genes Dev. 2006 Dec 15;20(24):3347-65 - PubMed
  49. J Proteomics. 2012 Dec 21;77:1-13 - PubMed
  50. J Biomed Biotechnol. 2010;2010:258494 - PubMed
  51. Science. 2002 May 31;296(5573):1655-7 - PubMed
  52. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3059-63 - PubMed
  53. Nature. 1994 Aug 4;370(6488):341-7 - PubMed
  54. Circ Res. 2008 Jun 6;102(11):1389-97 - PubMed
  55. FASEB J. 1998 Sep;12(12):1191-9 - PubMed
  56. J Biol Chem. 2002 Jan 11;277(2):1261-7 - PubMed
  57. Circ Res. 2005 Apr 15;96(7):756-66 - PubMed
  58. Circulation. 2006 Aug 1;114(5):388-96 - PubMed

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