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Soligo D, Balercia G, Osculati F, et al. The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study. Leuk Lymphoma. 1989;1(1):29-34doi: 10.3109/10428198909042455.
Soligo, D., Balercia, G., Osculati, F., Quirici, N., Sbarbati, A., Harven, E. D., & Deliliers, G. L. (1989). The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study. Leukemia & lymphoma, 1(1), 29-34. https://doi.org/10.3109/10428198909042455
Soligo, D, et al. "The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study." Leukemia & lymphoma vol. 1,1 (1989): 29-34. doi: https://doi.org/10.3109/10428198909042455
Soligo D, Balercia G, Osculati F, Quirici N, Sbarbati A, Harven ED, Deliliers GL. The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study. Leuk Lymphoma. 1989;1(1):29-34. doi: 10.3109/10428198909042455. PMID: 27456404.
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Leuk Lymphoma. 1989;1(1):29-34. doi: 10.3109/10428198909042455.

The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study.

Leukemia & lymphoma

D Soligo, G Balercia, F Osculati, N Quirici, A Sbarbati, E D Harven, G L Deliliers

Affiliations

  1. a Istituto di Scienze Mediche, University of Milano, Italy.
  2. b Istituto di Anatomia Umana ed Istologia, University of Verona, Italy.
  3. c Department of Pathology, University of Toronto, Canada.

PMID: 27456404 DOI: 10.3109/10428198909042455

Abstract

Bone-marrow cells from 11 cases of T- and 18 cases of B-lymphatic leukemia, at different maturation stages, were examined by scanning electron microscope (SEMI. All cases were extensively studied for the expression of surface markers by immunofluorescence. In addition six cases of T- and 10 cases of B-cell leukemia were labeled with a panel of monoclon;tl antibodies (including CD3, CD5, CD7, CD10, CD4, CD8, CD19, CD20 and CD22) and, after incubation with a colloidal gold conjugate, observed with SEM in the back-scattered electron imaging mode. Early stages of leukemic lymphoid B- and T-cell differentiation are characterized by prevalently smooth cell surfaces. Short stub-like microvilli constantly appear on more mature T cells, while complex surface features like small ruffles and pleomorphic microvilli are present in well-differentiated B-cell proliferations. Surface microvilli can be interpreted als structural features of lymphoid cells, progressively expressed with maturation and differentiation of leukemic as well as normal cells.

Keywords: Lymphatic leukemia; monoclonal antibodies; scanning electron microscopy

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