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Maggi E, Parronchi P, Macchia D, et al. CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal. Leuk Lymphoma. 1990;2(3):215-22doi: 10.3109/10428199009053526.
Maggi, E., Parronchi, P., Macchia, D., Borrello, M. G., Biswas, P., Biassoni, D., Simonelli, C., Bellesi, G., Gallo, O., Pierotti, & Romagnani, S. (1990). CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal. Leukemia & lymphoma, 2(3), 215-22. https://doi.org/10.3109/10428199009053526
Maggi, E, et al. "CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal." Leukemia & lymphoma vol. 2,3 (1990): 215-22. doi: https://doi.org/10.3109/10428199009053526
Maggi E, Parronchi P, Macchia D, Borrello MG, Biswas P, Biassoni D, Simonelli C, Bellesi G, Gallo O, Pierotti, Romagnani S. CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal. Leuk Lymphoma. 1990;2(3):215-22. doi: 10.3109/10428199009053526. PMID: 27456737.
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Leuk Lymphoma. 1990;2(3):215-22. doi: 10.3109/10428199009053526.

CD4 + T Cells from Lymph Nodes Involved by Hodgkin's Disease Showing Response in Autologous Mixed Lymphocyte Reaction, are Polyclonal.

Leukemia & lymphoma

E Maggi, P Parronchi, D Macchia, M G Borrello, P Biswas, D Biassoni, C Simonelli, G Bellesi, O Gallo, Pierotti, S Romagnani

Affiliations

  1. a Clinical Immunology Department, University of Florence, Florence.
  2. b Experimental Oncology Division, National Institute for Cancer Research and Therapy, Milan, Italy.
  3. c Haematology Departmen, University of Florence, Florence.
  4. d Othorynolaryngology Departments, University of Florence, Florence.

PMID: 27456737 DOI: 10.3109/10428199009053526

Abstract

Thirteen CD4 + T cell clones (TCCs) showing autologous mixed lymphocyte reaction (AMLR) and 12 CD4 + AMLR-negative TCCs derived from involved lymph nodes (LN) of 4 untreated patients with newly-diagnosed Hodgkin's disease (HD) were analyzed for some functional activities and for T cell receptor (TCR) gamma and beta gene rearrangement The majority of the AMLR-positive-, but only two AMLR-negative TCCs showed cytolytic potential when assayed by a lectin-dependent assay. In addition, the proportion of clones able to produce interleukin 2 (IL-2) was higher among AMLR-positive- than AMLR-negative TCCs and the amount of IL-2 synthesized by AMLR-positive TCCs was significantly greater than that of AMLR-negative TCCs. In contrast, no difference in the profile of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) production between AMLR-positive- and AMLR-negative TCCs was detected When AMLR-positive TCCs were analysed for their TCR beta and gamma gene rearrangements, a highly heterogeneous pattern was found. More importantly, TCCs derived from the same donor and displaying the same kind of TCR beta and gamma gene rearrangement showed different patterns of rearrangements, suggesting that CD4 + cells from LN involved by HD, displaying such an unusual functional profile, are polyclonal.

Keywords: Hodgkin's Disease; T cell clones; TCR beta and gamma gene rearrangements; autoreactive T cells

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