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Motoji T, Takanashi M, Masuda M, et al. Colony Stimulating Activities of GM-CSF, G-CSF and IL-3 on Blast Progenitors from Acute Myeloblastic Leukemia. Leuk Lymphoma. 1990;2(6):407-14doi: 10.3109/10428199009069294.
Motoji, T., Takanashi, M., Masuda, M., Nakayama, K., Oshimi, K., & Mizoguchi, H. (1990). Colony Stimulating Activities of GM-CSF, G-CSF and IL-3 on Blast Progenitors from Acute Myeloblastic Leukemia. Leukemia & lymphoma, 2(6), 407-14. https://doi.org/10.3109/10428199009069294
Motoji, T, et al. "Colony Stimulating Activities of GM-CSF, G-CSF and IL-3 on Blast Progenitors from Acute Myeloblastic Leukemia." Leukemia & lymphoma vol. 2,6 (1990): 407-14. doi: https://doi.org/10.3109/10428199009069294
Motoji T, Takanashi M, Masuda M, Nakayama K, Oshimi K, Mizoguchi H. Colony Stimulating Activities of GM-CSF, G-CSF and IL-3 on Blast Progenitors from Acute Myeloblastic Leukemia. Leuk Lymphoma. 1990;2(6):407-14. doi: 10.3109/10428199009069294. PMID: 27457045.
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Leuk Lymphoma. 1990;2(6):407-14. doi: 10.3109/10428199009069294.

Colony Stimulating Activities of GM-CSF, G-CSF and IL-3 on Blast Progenitors from Acute Myeloblastic Leukemia.

Leukemia & lymphoma

T Motoji, M Takanashi, M Masuda, K Nakayama, K Oshimi, H Mizoguchi

Affiliations

  1. a Division of Hematology, Department of Medicine, Tokyo Women's Medical College, Tokyo, Japan.

PMID: 27457045 DOI: 10.3109/10428199009069294

Abstract

The colony stimulating activities (CSAs) of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF), recombinant granulocyte colony-stimulating factor (rG-CSF) and recombinant interleukin-3 (rIL-3) on blast cells from various types of acute myeloblastic leukemia (AML) patients were studied using an in vitro blast colony assay. The stimulatory magnitude of rGM-CSF was highest in type M4 blasts but almost non-existent in type M1 blasts. Some preferential stimulatory action of rG-CSF was observed in type M2, M4 blasts and in overt leukemia in patients with refractory anemia with excess of blasts. On the other hand, the stimulatory activity of rIL-3 did not differ much with the AML subtype. Exposure to rGM-CSF, rG-CSF or rIL-3 did not alter the cellular phenotype or morphology of the leukemic blasts or the self-renewal capacity of blast progenitors. Simultaneous addition of rGM-CSF, rG-CSF and rIL-3, in various combinations, produced variable results and a simple additive effect was seen in 40% of the cases studied and either a significantly larger or smaller value in the remaining cases. Recombinant GM-CSF, rG-CSF and rIL-3 when added together did not reconstitute CSA of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM).

Keywords: AML subtype; G-CSF; GM-CSF; IL-3; L-CFU

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