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Bohloli M, Atashi A, Soleimani M, et al. Investigating Effects of Acidic pH on Proliferation, Invasion and Drug-Induced Apoptosis in Lymphoblastic Leukemia. Cancer Microenviron. 2016;9(2):119-126doi: 10.1007/s12307-016-0187-0.
Bohloli, M., Atashi, A., Soleimani, M., Kaviani, S., & Anbarlou, A. (2016). Investigating Effects of Acidic pH on Proliferation, Invasion and Drug-Induced Apoptosis in Lymphoblastic Leukemia. Cancer microenvironment : official journal of the International Cancer Microenvironment Society, 9(2), 119-126. https://doi.org/10.1007/s12307-016-0187-0
Bohloli, Mahbobeh, et al. "Investigating Effects of Acidic pH on Proliferation, Invasion and Drug-Induced Apoptosis in Lymphoblastic Leukemia." Cancer microenvironment : official journal of the International Cancer Microenvironment Society vol. 9,2 (2016): 119-126. doi: https://doi.org/10.1007/s12307-016-0187-0
Bohloli M, Atashi A, Soleimani M, Kaviani S, Anbarlou A. Investigating Effects of Acidic pH on Proliferation, Invasion and Drug-Induced Apoptosis in Lymphoblastic Leukemia. Cancer Microenviron. 2016 Dec;9(2):119-126. doi: 10.1007/s12307-016-0187-0. Epub 2016 Jul 25. PMID: 27457339; PMCID: PMC5264660.
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Cancer Microenviron. 2016 Dec;9(2):119-126. doi: 10.1007/s12307-016-0187-0. Epub 2016 Jul 25.

Investigating Effects of Acidic pH on Proliferation, Invasion and Drug-Induced Apoptosis in Lymphoblastic Leukemia.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society

Mahbobeh Bohloli, Amir Atashi, Masoud Soleimani, Saeid Kaviani, Azadeh Anbarlou

Affiliations

  1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  2. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. [email protected].

PMID: 27457339 PMCID: PMC5264660 DOI: 10.1007/s12307-016-0187-0

Abstract

Some studies have shown that extracellular pH in tumors, which results in tumor progression, is less than that in normal tissues. The aim of this study was to investigate the effects of extracellular acidic pH on proliferation, invasion, and drug-induced apoptosis in acute lymphoblastic cells. The cells were cultured in different pH (pH 6.6 and pH 7.4) for 12 days. Cell proliferation was assessed by MTT assay and cell invasion was assayed by invasion assay and gene expression analysis of MMP-9. Drug-induced apoptosis was evaluated after exposure to doxorubicin for 24 hours by annexin V/PI staining and gene expression analysis of BAX pro-apoptotic protein. The results indicated the enhanced growth and invasion of leukemic cells at pH 6.6 (P ≤ 0.05). Furthermore, the cells at pH 6.6 were resistant to apoptosis by doxorubicin (P ≤ 0.05). It can be concluded that acidic pH increases the proliferation, invasion and reduces the drug-induced apoptosis in acute lymphoblastic leukemia. Extracellular acidity can influence the behavior of leukemic cells and therefore, the manipulation of extracellular liquid can be selected as a therapeutic strategy for leukemia, especially for acute lymphoblastic leukemia.

Keywords: Acidic pH; Drug-induced apoptosis; Invasion; Lymphoblastic leukemia; Proliferation

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