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Lamon S, Zacharewicz E, Arentson-Lantz E, et al. Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults. Front Physiol. 2016;7:292doi: 10.3389/fphys.2016.00292.
Lamon, S., Zacharewicz, E., Arentson-Lantz, E., Gatta, P. A., Ghobrial, L., Gerlinger-Romero, F., Garnham, A., Paddon-Jones, D., & Russell, A. P. (2016). Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults. Frontiers in physiology, 7292. https://doi.org/10.3389/fphys.2016.00292
Lamon, Séverine, et al. "Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults." Frontiers in physiology vol. 7 (2016): 292. doi: https://doi.org/10.3389/fphys.2016.00292
Lamon S, Zacharewicz E, Arentson-Lantz E, Gatta PA, Ghobrial L, Gerlinger-Romero F, Garnham A, Paddon-Jones D, Russell AP. Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults. Front Physiol. 2016 Jul 08;7:292. doi: 10.3389/fphys.2016.00292. eCollection 2016. PMID: 27458387; PMCID: PMC4937030.
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Front Physiol. 2016 Jul 08;7:292. doi: 10.3389/fphys.2016.00292. eCollection 2016.

Erythropoietin Does Not Enhance Skeletal Muscle Protein Synthesis Following Exercise in Young and Older Adults.

Frontiers in physiology

Séverine Lamon, Evelyn Zacharewicz, Emily Arentson-Lantz, Paul A Della Gatta, Lobna Ghobrial, Frederico Gerlinger-Romero, Andrew Garnham, Douglas Paddon-Jones, Aaron P Russell

Affiliations

  1. Institute for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University Geelong, VIC, Australia.
  2. Department of Nutrition and Metabolism, University of Texas Medical Branch Galveston, TX, USA.

PMID: 27458387 PMCID: PMC4937030 DOI: 10.3389/fphys.2016.00292

Abstract

PURPOSE: Erythropoietin (EPO) is a renal cytokine that is primarily involved in hematopoiesis while also playing a role in non-hematopoietic tissues expressing the EPO-receptor (EPOR). The EPOR is present in human skeletal muscle. In mouse skeletal muscle, EPO stimulation can activate the AKT serine/threonine kinase 1 (AKT) signaling pathway, the main positive regulator of muscle protein synthesis. We hypothesized that a single intravenous EPO injection combined with acute resistance exercise would have a synergistic effect on skeletal muscle protein synthesis via activation of the AKT pathway.

METHODS: Ten young (24.2 ± 0.9 years) and 10 older (66.6 ± 1.1 years) healthy subjects received a primed, constant infusion of [ring-13C(6)] L-phenylalanine and a single injection of 10,000 IU epoetin-beta or placebo in a double-blind randomized, cross-over design. 2 h after the injection, the subjects completed an acute bout of leg extension resistance exercise to stimulate skeletal muscle protein synthesis.

RESULTS: Significant interaction effects in the phosphorylation levels of the members of the AKT signaling pathway indicated a differential activation of protein synthesis signaling in older subjects when compared to young subjects. However, EPO offered no synergistic effect on vastus lateralis mixed muscle protein synthesis rate in young or older subjects.

CONCLUSIONS: Despite its ability to activate the AKT pathway in skeletal muscle, an acute EPO injection had no additive or synergistic effect on the exercise-induced activation of muscle protein synthesis or muscle protein synthesis signaling pathways.

Keywords: aging; anabolic signaling; erythropoietin; muscle protein synthesis; resistance exercise

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