Display options
Cite
Freitas MS, Oliveira AF, da Silva TA, et al. Paracoccin Induces M1 Polarization of Macrophages via Interaction with TLR4. Front Microbiol. 2016;7:1003doi: 10.3389/fmicb.2016.01003.
Freitas, M. S., Oliveira, A. F., da Silva, T. A., Fernandes, F. F., Gonçales, R. A., Almeida, F., & Roque-Barreira, M. C. (2016). Paracoccin Induces M1 Polarization of Macrophages via Interaction with TLR4. Frontiers in microbiology, 71003. https://doi.org/10.3389/fmicb.2016.01003
Freitas, Mateus S, et al. "Paracoccin Induces M1 Polarization of Macrophages via Interaction with TLR4." Frontiers in microbiology vol. 7 (2016): 1003. doi: https://doi.org/10.3389/fmicb.2016.01003
Freitas MS, Oliveira AF, da Silva TA, Fernandes FF, Gonçales RA, Almeida F, Roque-Barreira MC. Paracoccin Induces M1 Polarization of Macrophages via Interaction with TLR4. Front Microbiol. 2016 Jul 05;7:1003. doi: 10.3389/fmicb.2016.01003. eCollection 2016. PMID: 27458431; PMCID: PMC4932198.
Copy Download .nbib Format: NLM
Share it on

Front Microbiol. 2016 Jul 05;7:1003. doi: 10.3389/fmicb.2016.01003. eCollection 2016.

Paracoccin Induces M1 Polarization of Macrophages via Interaction with TLR4.

Frontiers in microbiology

Mateus S Freitas, Aline F Oliveira, Thiago A da Silva, Fabrício F Fernandes, Relber A Gonçales, Fausto Almeida, Maria C Roque-Barreira

Affiliations

  1. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo Ribeirão Preto, Brazil.

PMID: 27458431 PMCID: PMC4932198 DOI: 10.3389/fmicb.2016.01003

Abstract

The fungal human pathogen Paracoccidioides brasiliensis contains paracoccin (PCN), a multi-domain protein that has lectin and N-acetyl-glucosaminidase activities, which account for its effects on the growth and morphogenesis of the fungus and on the activation of host macrophages through its interaction with TLR N-glycans. With the purpose of detailing the knowledge on the effects of PCN on macrophages, we used recombinant PCN expressed in Pichia pastoris (p-rPCN) to stimulate isolated murine peritoneal macrophages. The activation of these cells manifested through the release of high levels of inflammatory mediators, such as nitric oxide, TNF-α, IL-12p40, and IL-6. Furthermore, peritoneal macrophages stimulated with p-rPCN increased the relative expression of STAT1, SOCS3, and iNOS2 mRNA (M1 polarization markers). However, the expression of Arginase-1, Ym-1, and FIZZ1 (M2 polarization markers) remained at basal levels. Interestingly, the observed M1 macrophages' polarization triggered by p-rPCN was abolished in cells obtained from knockout Toll-like receptor-4 mice. In this case, the p-rPCN-induced production of pro-inflammatory mediators was blocked too. These results demonstrate that the classical activation of macrophages induced by paracoccin depends on TLR4. Taken together, the results of our study indicate that paracoccin acts as a TLR agonist able to modulate immunity and exerts biological activities that favor its applicability as an immunotherapeutic agent to combat systemic fungal infections.

Keywords: TLR; lectin; macrophage polarization; paracoccin; proinflammatory mediators

References

  1. J Immunol. 2009 Jul 15;183(2):1279-90 - PubMed
  2. Crit Rev Oral Biol Med. 2003;14(5):377-83 - PubMed
  3. Yeast. 2005 Mar;22(4):249-70 - PubMed
  4. PLoS One. 2015 Nov 17;10(11):e0143087 - PubMed
  5. J Clin Invest. 2012 Mar;122(3):787-95 - PubMed
  6. Nat Rev Immunol. 2014 Feb;14(2):81-93 - PubMed
  7. Microbes Infect. 2006 Mar;8(3):704-13 - PubMed
  8. Am J Pathol. 2010 Feb;176(2):774-85 - PubMed
  9. Methods Mol Biol. 2012;824:329-58 - PubMed
  10. Mycopathologia. 1985 Dec;92(3):179-88 - PubMed
  11. J Leukoc Biol. 2008 May;83(5):1088-99 - PubMed
  12. PLoS One. 2015 Dec 10;10 (12 ):e0144507 - PubMed
  13. Infect Immun. 2010 Mar;78(3):1078-88 - PubMed
  14. Rev Inst Med Trop Sao Paulo. 2005 Sep-Oct;47(5):257-62 - PubMed
  15. Yeast. 2010 Feb;27(2):67-76 - PubMed
  16. PLoS One. 2011;6(12):e29216 - PubMed
  17. PLoS Negl Trop Dis. 2014 Apr 17;8(4):e2788 - PubMed
  18. Braz J Infect Dis. 2005 Apr;9(2):126-33 - PubMed
  19. PLoS One. 2013;8(1):e54845 - PubMed
  20. Avicenna J Med Biotechnol. 2010 Oct;2(4):197-206 - PubMed
  21. Anal Biochem. 1982 Oct;126(1):131-8 - PubMed
  22. PLoS Negl Trop Dis. 2014 Dec 04;8(12):e3317 - PubMed
  23. Mycopathologia. 2008 Apr-May;165(4-5):223-36 - PubMed
  24. Microbes Infect. 2007 May;9(6):695-703 - PubMed
  25. Nat Rev Immunol. 2011 Oct 14;11(11):723-37 - PubMed
  26. Rev Iberoam Micol. 2007 Dec 31;24(4):249-58 - PubMed
  27. Am J Trop Med Hyg. 1993 May;48(5):626-9 - PubMed
  28. Semin Respir Crit Care Med. 2008 Apr;29(2):182-97 - PubMed
  29. Med Mycol. 2000 Aug;38(4):317-22 - PubMed
  30. MBio. 2013 Jun 18;4(3):e00264-13 - PubMed
  31. Nat Rev Immunol. 2011 Oct 25;11(11):750-61 - PubMed

Publication Types