Display options
Cite
Rothrauff BB, Numpaisal PO, Lauro BB, et al. Augmented repair of radial meniscus tear with biomimetic electrospun scaffold: an in vitro mechanical analysis. J Exp Orthop. 2016;3(1):23doi: 10.1186/s40634-016-0058-0.
Rothrauff, B. B., Numpaisal, P. O., Lauro, B. B., Alexander, P. G., Debski, R. E., Musahl, V., & Tuan, R. S. (2016). Augmented repair of radial meniscus tear with biomimetic electrospun scaffold: an in vitro mechanical analysis. Journal of experimental orthopaedics, 3(1), 23. https://doi.org/10.1186/s40634-016-0058-0
Rothrauff, Benjamin B, et al. "Augmented repair of radial meniscus tear with biomimetic electrospun scaffold: an in vitro mechanical analysis." Journal of experimental orthopaedics vol. 3,1 (2016): 23. doi: https://doi.org/10.1186/s40634-016-0058-0
Rothrauff BB, Numpaisal PO, Lauro BB, Alexander PG, Debski RE, Musahl V, Tuan RS. Augmented repair of radial meniscus tear with biomimetic electrospun scaffold: an in vitro mechanical analysis. J Exp Orthop. 2016 Dec;3(1):23. doi: 10.1186/s40634-016-0058-0. Epub 2016 Sep 13. PMID: 27624439; PMCID: PMC5021645.
Copy Download .nbib Format: NLM
Share it on

J Exp Orthop. 2016 Dec;3(1):23. doi: 10.1186/s40634-016-0058-0. Epub 2016 Sep 13.

Augmented repair of radial meniscus tear with biomimetic electrospun scaffold: an in vitro mechanical analysis.

Journal of experimental orthopaedics

Benjamin B Rothrauff, Piya-On Numpaisal, Brian B Lauro, Peter G Alexander, Richard E Debski, Volker Musahl, Rocky S Tuan

Affiliations

  1. Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 450 Technology Drive, Room 221, Pittsburgh, PA, 15219, USA.
  2. McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, 15219, USA.
  3. College of Medicine, National Taiwan University, Taipei, Taiwan.
  4. Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
  5. Orthopaedic Robotics Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 300 Technology Drive, Pittsburgh, PA, USA.
  6. Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 450 Technology Drive, Room 221, Pittsburgh, PA, 15219, USA. [email protected].
  7. Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA. [email protected].
  8. McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, 15219, USA. [email protected].

PMID: 27624439 PMCID: PMC5021645 DOI: 10.1186/s40634-016-0058-0

Abstract

BACKGROUND: Large radial tears that disrupt the circumferential fibers of the meniscus are associated with reduced meniscal function and increased risk of joint degeneration. Electrospun fibrous scaffolds can mimic the topography and mechanics of fibrocartilaginous tissues and simultaneously serve as carriers of cells and growth factors, yet their incorporation into clinically relevant suture repair techniques for radial meniscus tears is unexplored. The purposes of this study were to (1) evaluate the effect of fiber orientation on the tensile properties and suture-retention strength of multilayered electrospun scaffolds and (2) determine the mechanical effects of scaffold inclusion within a surgical repair of a simulated radial meniscal tear. The experimental hypothesis was that augmentation with a multilayered scaffold would not compromise the strength of the repair.

METHODS: Three multilayered electrospun scaffolds with different fiber orientations were fabricated-aligned, random, and biomimetic. The biomimetic scaffold was comprised of four layers in the following order (deep to superficial)-aligned longitudinal, aligned transverse, aligned longitudinal, and random-respectively corresponding to circumferential, radial, circumferential, and superficial collagen fibers of the native meniscus. Material properties (i.e., ultimate stress, modulus, etc.) of the scaffolds were determined in the parallel and perpendicular directions, as was suture retention strength. Complete radial tears of lateral bovine meniscus explants were repaired with a double horizontal mattress suture technique, with or without inclusion of the biomimetic scaffold sheath. Both repair groups, as well as native controls, were cyclically loaded between 5 and 20 N for 500 cycles and then loaded to failure. Clamp-to-clamp distance (i.e., residual elongation) was measured following various cycles. Ultimate load, ultimate elongation, and stiffness, were also determined. Group differences were evaluated by one-way ANOVA or Student's t-test where appropriate.

RESULTS: Aligned scaffolds possessed the most anisotropic mechanical properties, whereas random scaffolds showed uniform properties in the parallel and perpendicular directions. In comparison, the biomimetic scaffold possessed moduli in the parallel (68.7 ± 14.7 MPa) and perpendicular (39.4 ± 11.6 MPa) directions that respectively approximate the reported circumferential and radial tensile properties of native menisci. The ultimate suture retention load of the biomimetic scaffold in the parallel direction (7.2 ± 1.6 N) was significantly higher than all other conditions (p < 0.001). Biomimetic scaffold augmentation did not compromise mechanical properties when compared against suture repair in terms of residual elongation after 500 cycles (scaffold: 5.05 ± 0.89 mm vs. repair: 4.78 ± 1.24 mm), ultimate failure load (137.1 ± 31.0 N vs. 124.4 ± 21.4 N), ultimate elongation (12.09 ± 5.89 mm vs. 10.14 ± 4.61 mm), and stiffness (20.8 ± 3.6 vs. 18.4 ± 4.7 N/mm).

CONCLUSIONS: While multilayered scaffold sheets were successfully fabricated to mimic the ultrastructure and anisotropic tensile properties of native menisci, improvements in suture retention strength or adoption of superior surgical techniques will be needed to further enhance the mechanical strength of repairs of radial meniscal tears.

Keywords: Meniscus repair; Radial tear; Scaffold

References

  1. Tissue Eng Part A. 2016 Mar;22(5-6):436-48 - PubMed
  2. J Bone Joint Surg Am. 2011 Jun 15;93(12 ):1089-95 - PubMed
  3. Am J Sports Med. 2016 Mar;44(3):639-45 - PubMed
  4. Stem Cell Res Ther. 2015 Apr 30;6:86 - PubMed
  5. Am J Sports Med. 2015 Sep;43(9):2270-6 - PubMed
  6. Am J Sports Med. 2012 Aug;40(8):1863-70 - PubMed
  7. Am J Sports Med. 2013 Oct;41(10):2333-9 - PubMed
  8. J Orthop Res. 2015 Apr;33(4):572-83 - PubMed
  9. J Orthop Res. 1989;7(6):771-82 - PubMed
  10. Arthroscopy. 2012 Dec;28(12 ):1873-81 - PubMed
  11. Arthroscopy. 2012 Mar;28(3):372-81 - PubMed
  12. Acta Biomater. 2015 Oct;26:124-35 - PubMed
  13. Am J Sports Med. 1983 May-Jun;11(3):131-41 - PubMed
  14. J Shoulder Elbow Surg. 2010 Jul;19(5):688-96 - PubMed
  15. J Shoulder Elbow Surg. 2012 Dec;21(12 ):1680-6 - PubMed
  16. Biomaterials. 2011 Oct;32(30):7411-31 - PubMed
  17. Biomaterials. 2014 Aug;35(25):6907-17 - PubMed
  18. Am J Sports Med. 2010 Dec;38(12 ):2472-6 - PubMed
  19. Clin Anat. 2015 Mar;28(2):269-87 - PubMed
  20. Arthroscopy. 2015 Feb;31(2):293-8 - PubMed
  21. Am J Sports Med. 2010 Nov;38(11):2281-7 - PubMed
  22. J Biomech. 1995 Apr;28(4):411-22 - PubMed
  23. Biomaterials. 2010 Aug;31(24):6190-200 - PubMed
  24. J Biomech. 2014 Jun 27;47(9):2183-8 - PubMed
  25. Am J Sports Med. 2012 Feb;40(2):414-8 - PubMed
  26. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14176-81 - PubMed
  27. Ann Biomed Eng. 2015 Mar;43(3):819-31 - PubMed
  28. Am J Sports Med. 1998 Jan-Feb;26(1):87-95 - PubMed
  29. J Orthop Res. 2013 Aug;31(8):1208-17 - PubMed
  30. J Bone Joint Surg Br. 1948 Nov;30B(4):664-70 - PubMed
  31. HSS J. 2011 Jul;7(2):157-63 - PubMed
  32. J Orthop Res. 2000 Nov;18(6):945-51 - PubMed
  33. J Biomech. 2015 Jun 1;48(8):1412-9 - PubMed
  34. Knee. 2012 Aug;19(4):493-9 - PubMed
  35. J Shoulder Elbow Surg. 2012 Aug;21(8):1064-71 - PubMed
  36. Tissue Eng Part A. 2015 Jul;21(13-14):2066-75 - PubMed
  37. Biomaterials. 2015 Jan;39:85-94 - PubMed
  38. Arthroscopy. 2015 May;31(5):944-55 - PubMed
  39. Cartilage. 2016 Apr;7(2):123-39 - PubMed
  40. Clin Orthop Relat Res. 1990 Mar;(252):19-31 - PubMed
  41. Am J Sports Med. 1989 Mar-Apr;17(2):164-75 - PubMed
  42. Sci Transl Med. 2014 Dec 10;6(266):266ra171 - PubMed
  43. Am J Sports Med. 1991 Nov-Dec;19(6):626-31 - PubMed
  44. Arthroscopy. 2012 Aug;28(8):1124-1134.e2 - PubMed

Publication Types