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Lan H, Sun R, Fan K, et al. The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity. Front Microbiol. 2016;7:1324doi: 10.3389/fmicb.2016.01324.
Lan, H., Sun, R., Fan, K., Yang, K., Zhang, F., Nie, X. Y., Wang, X., Zhuang, Z., & Wang, S. (2016). The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity. Frontiers in microbiology, 71324. https://doi.org/10.3389/fmicb.2016.01324
Lan, Huahui, et al. "The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity." Frontiers in microbiology vol. 7 (2016): 1324. doi: https://doi.org/10.3389/fmicb.2016.01324
Lan H, Sun R, Fan K, Yang K, Zhang F, Nie XY, Wang X, Zhuang Z, Wang S. The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity. Front Microbiol. 2016 Aug 30;7:1324. doi: 10.3389/fmicb.2016.01324. eCollection 2016. PMID: 27625637; PMCID: PMC5003836.
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Front Microbiol. 2016 Aug 30;7:1324. doi: 10.3389/fmicb.2016.01324. eCollection 2016.

The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity.

Frontiers in microbiology

Huahui Lan, Ruilin Sun, Kun Fan, Kunlong Yang, Feng Zhang, Xin Y Nie, Xiunai Wang, Zhenhong Zhuang, Shihua Wang

Affiliations

  1. Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University Fuzhou, China.

PMID: 27625637 PMCID: PMC5003836 DOI: 10.3389/fmicb.2016.01324

Abstract

Histone acetyltransferases (HATs) help regulate fungal development and the production of secondary metabolites. In this study, we determined that the HAT AflGcnE influenced morphogenesis and aflatoxin biosynthesis in Aspergillus flavus. We observed that AflGcnE localized to the nucleus and cytoplasm during the conidial production and germination stages, while it was located mainly in the nucleus during the hyphal development stage. Deletion of AflgcnE inhibited the growth of A. flavus and decreased the hydrophobicity of the cell surface. The ΔAflgcnE mutant exhibited a lack of asexual sporulation and was unable to generate sclerotia. Additionally, AflgcnE was required to maintain cell wall integrity and genotoxic stress responses. Importantly, the ΔAflgcnE mutant did not produce aflatoxins, which was consistent with a significant down-regulation of aflatoxin gene expression levels. Furthermore, our data revealed that AflgcnE is a pathogenicity factor required for colonizing maize seeds. In summary, we revealed that A. flavus AflGcnE is crucial for morphological development, aflatoxin biosynthesis, stress responses, and pathogenicity. Our findings help clarify the functional divergence of GcnE orthologs, and may provide a possible target for controlling A. flavus infections of agriculturally important crops.

Keywords: A. flavus; AflgcnE; aflatoxin; histone acetyltransferase; pathogenicity

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