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Rahi P, Prakash O, Shouche YS. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists. Front Microbiol. 2016;7:1359doi: 10.3389/fmicb.2016.01359.
Rahi, P., Prakash, O., & Shouche, Y. S. (2016). Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists. Frontiers in microbiology, 71359. https://doi.org/10.3389/fmicb.2016.01359
Rahi, Praveen, et al. "Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists." Frontiers in microbiology vol. 7 (2016): 1359. doi: https://doi.org/10.3389/fmicb.2016.01359
Rahi P, Prakash O, Shouche YS. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists. Front Microbiol. 2016 Aug 30;7:1359. doi: 10.3389/fmicb.2016.01359. eCollection 2016. PMID: 27625644; PMCID: PMC5003876.
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Front Microbiol. 2016 Aug 30;7:1359. doi: 10.3389/fmicb.2016.01359. eCollection 2016.

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists.

Frontiers in microbiology

Praveen Rahi, Om Prakash, Yogesh S Shouche

Affiliations

  1. Microbial Culture Collection, National Centre for Cell Science Pune, India.

PMID: 27625644 PMCID: PMC5003876 DOI: 10.3389/fmicb.2016.01359

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) based biotyping is an emerging technique for high-throughput and rapid microbial identification. Due to its relatively higher accuracy, comprehensive database of clinically important microorganisms and low-cost compared to other microbial identification methods, MALDI-TOF MS has started replacing existing practices prevalent in clinical diagnosis. However, applicability of MALDI-TOF MS in the area of microbial ecology research is still limited mainly due to the lack of data on non-clinical microorganisms. Intense research activities on cultivation of microbial diversity by conventional as well as by innovative and high-throughput methods has substantially increased the number of microbial species known today. This important area of research is in urgent need of rapid and reliable method(s) for characterization and de-replication of microorganisms from various ecosystems. MALDI-TOF MS based characterization, in our opinion, appears to be the most suitable technique for such studies. Reliability of MALDI-TOF MS based identification method depends mainly on accuracy and width of reference databases, which need continuous expansion and improvement. In this review, we propose a common strategy to generate MALDI-TOF MS spectral database and advocated its sharing, and also discuss the role of MALDI-TOF MS based high-throughput microbial identification in microbial ecology studies.

Keywords: MALDI-TOF mass spectrometry; culturomics; high-throughput identifications; microbial identification; spectral database

References

  1. J Clin Microbiol. 2010 Oct;48(10):3482-6 - PubMed
  2. ISME J. 2016 Dec;10 (12 ):2801-2816 - PubMed
  3. PLoS One. 2011;6(5):e20223 - PubMed
  4. PLoS One. 2011;6(12):e28425 - PubMed
  5. BMC Res Notes. 2014 Jun 02;7:330 - PubMed
  6. J Clin Microbiol. 2010 Mar;48(3):900-7 - PubMed
  7. J Clin Microbiol. 2011 Feb;49(2):693-6 - PubMed
  8. J Microbiol Methods. 2015 Feb;109:93-105 - PubMed
  9. J Proteomics. 2013 Jan 14;78:273-80 - PubMed
  10. J Microbiol Methods. 2013 Sep;94(3):390-6 - PubMed
  11. Front Genet. 2016 Feb 10;7:4 - PubMed
  12. Sci Rep. 2015 Nov 06;5:16326 - PubMed
  13. J Microbiol Methods. 2012 May;89(2):133-6 - PubMed
  14. Nat Rev Microbiol. 2013 Aug;11(8):574-85 - PubMed
  15. PLoS One. 2008 Jul 30;3(7):e2843 - PubMed
  16. Med Microbiol Immunol. 2014 Apr;203(2):93-9 - PubMed
  17. Clin Microbiol Infect. 2012 Dec;18(12):1157-9 - PubMed
  18. PLoS One. 2014 May 01;9(5):e87419 - PubMed
  19. J Clin Microbiol. 2012 Apr;50(4):1415-8 - PubMed
  20. Clin Infect Dis. 2013 Aug;57(4):564-72 - PubMed
  21. PLoS One. 2012;7(6):e38515 - PubMed
  22. J Clin Microbiol. 2013 Jul;51(7):2225-31 - PubMed
  23. Mass Spectrom Rev. 2001 Jul-Aug;20(4):157-71 - PubMed
  24. J Clin Microbiol. 2011 Aug;49(8):2868-73 - PubMed
  25. Clin Infect Dis. 2009 Aug 15;49(4):543-51 - PubMed
  26. J Clin Microbiol. 2014 May;52(5):1453-8 - PubMed
  27. Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2878-83 - PubMed
  28. Anal Biochem. 2011 Apr 1;411(1):122-8 - PubMed
  29. Antonie Van Leeuwenhoek. 2013 Mar;103(3):603-15 - PubMed
  30. J Microbiol Methods. 2015 May;112:24-7 - PubMed
  31. Appl Microbiol Biotechnol. 2014 Jun;98(12):5719-35 - PubMed
  32. Electrophoresis. 2012 Jul;33(14):2138-42 - PubMed
  33. Sci Rep. 2015 Nov 05;5:15834 - PubMed
  34. J Clin Microbiol. 2013 Nov;51(11):3711-6 - PubMed
  35. Swiss Med Wkly. 2010 Sep 24;140:w13095 - PubMed
  36. Appl Microbiol Biotechnol. 2005 Jun;67(4):539-48 - PubMed
  37. Anaerobe. 2011 Aug;17(4):211-2 - PubMed
  38. Appl Environ Microbiol. 2006 Feb;72(2):1180-9 - PubMed
  39. Int J Syst Evol Microbiol. 2015 Nov;65(11):4032-4042 - PubMed
  40. Syst Appl Microbiol. 2011 Feb;34(1):2-11 - PubMed
  41. J Clin Microbiol. 2012 Dec;50(12):3862-6 - PubMed
  42. FEMS Microbiol Lett. 2012 Mar;328(1):46-53 - PubMed
  43. J Microbiol Methods. 2013 Jan;92(1):14-24 - PubMed
  44. FEMS Microbiol Rev. 2012 Mar;36(2):380-407 - PubMed
  45. Bioinformatics. 2014 May 1;30(9):1280-6 - PubMed
  46. Mass Spectrom Rev. 2013 May-Jun;32(3):188-217 - PubMed
  47. Bioinformatics. 2006 Sep 1;22(17):2059-65 - PubMed
  48. Clin Microbiol Rev. 2015 Jan;28(1):237-64 - PubMed
  49. Syst Appl Microbiol. 2015 Feb;38(1):16-25 - PubMed
  50. Syst Appl Microbiol. 2011 Feb;34(1):69-75 - PubMed
  51. Nucleic Acids Res. 2014 Jan;42(Database issue):D613-6 - PubMed
  52. BMC Res Notes. 2014 Dec 09;7:892 - PubMed
  53. Int J Antimicrob Agents. 2014 Aug;44(2):117-24 - PubMed
  54. Syst Appl Microbiol. 2013 Dec;36(8):565-71 - PubMed
  55. Anal Chim Acta. 2014 Aug 20;840:49-57 - PubMed
  56. J Mol Diagn. 2012 Sep;14(5):419-23 - PubMed
  57. J Vet Diagn Invest. 2014 Sep;26(5):635-9 - PubMed
  58. Science. 2002 May 10;296(5570):1127-9 - PubMed
  59. Anal Chem. 2001 Feb 15;73(4):746-50 - PubMed
  60. Acta Clin Belg. 2011 Jul-Aug;66(4):267-73 - PubMed
  61. Clin Microbiol Infect. 2012 Dec;18(12):1185-93 - PubMed
  62. J Clin Microbiol. 2012 Jun;50(6):2034-9 - PubMed
  63. J Clin Microbiol. 2013 Mar;51(3):828-34 - PubMed
  64. FEMS Microbiol Lett. 2010 Aug 1;309(1):1-7 - PubMed
  65. Syst Appl Microbiol. 2011 Feb;34(1):1 - PubMed
  66. Syst Appl Microbiol. 2015 Jun;38(4):266-75 - PubMed
  67. J Microbiol Methods. 2011 Sep;86(3):327-36 - PubMed
  68. Nat Protoc. 2009;4(5):732-42 - PubMed
  69. Am J Bot. 2007 May;94(5):799-808 - PubMed
  70. Clin Microbiol Infect. 2011 Nov;17(11):1735-9 - PubMed
  71. J Clin Microbiol. 2012 Apr;50(4):1313-25 - PubMed
  72. Food Microbiol. 2013 Apr;33(2):235-42 - PubMed
  73. J Clin Microbiol. 2010 Nov;48(11):4015-21 - PubMed
  74. Antonie Van Leeuwenhoek. 2015 May;107(5):1207-16 - PubMed
  75. PLoS One. 2012;7(10):e48441 - PubMed
  76. J Med Microbiol. 2010 Mar;59(Pt 3):295-301 - PubMed
  77. J Biotechnol. 2013 Jun 10;165(3-4):167-74 - PubMed
  78. Extremophiles. 2004 Aug;8(4):259-68 - PubMed
  79. J Clin Microbiol. 2010 Apr;48(4):1169-75 - PubMed
  80. Bioinformatics. 2012 Sep 1;28(17):2270-1 - PubMed
  81. Appl Environ Microbiol. 2008 Dec;74(24):7715-22 - PubMed
  82. J Clin Microbiol. 2013 Apr;51(4):1231-6 - PubMed
  83. J Clin Microbiol. 2013 Jul;51(7):2182-94 - PubMed
  84. Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):15-22 - PubMed
  85. Appl Microbiol Biotechnol. 2012 Feb;93(3):965-74 - PubMed
  86. Appl Environ Microbiol. 2011 Oct;77(19):6858-66 - PubMed
  87. Proteomics. 2011 Aug;11(15):3143-53 - PubMed
  88. Appl Environ Microbiol. 2005 Jan;71(1):58-64 - PubMed
  89. Food Microbiol. 2013 Jun;34(2):406-11 - PubMed
  90. Clin Microbiol Infect. 2010 Nov;16(11):1604-13 - PubMed
  91. J Bacteriol. 2012 Aug;194(16):4151-60 - PubMed
  92. Appl Microbiol Biotechnol. 2014 Apr;98(8):3737-52 - PubMed
  93. Appl Environ Microbiol. 2007 Mar;73(6):1899-907 - PubMed
  94. PLoS One. 2014 Feb 12;9(2):e88895 - PubMed
  95. Clin Microbiol Infect. 2014 Oct;20(10):1007-15 - PubMed

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