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Yoo da K, Lee SH. Effect of Lipopolysaccharide (LPS) Exposure on the Reproductive Organs of Immature Female Rats. Dev Reprod. 2016;20(2):113-21doi: 10.12717/DR.2016.20.2.113.
Yoo, d. a. . K., & Lee, S. H. (2016). Effect of Lipopolysaccharide (LPS) Exposure on the Reproductive Organs of Immature Female Rats. Development & reproduction, 20(2), 113-21. https://doi.org/10.12717/DR.2016.20.2.113
Yoo, Da Kyung, and Lee, Sung-Ho. "Effect of Lipopolysaccharide (LPS) Exposure on the Reproductive Organs of Immature Female Rats." Development & reproduction vol. 20,2 (2016): 113-21. doi: https://doi.org/10.12717/DR.2016.20.2.113
Yoo da K, Lee SH. Effect of Lipopolysaccharide (LPS) Exposure on the Reproductive Organs of Immature Female Rats. Dev Reprod. 2016 Jun;20(2):113-21. doi: 10.12717/DR.2016.20.2.113. PMID: 27660826; PMCID: PMC5027216.
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Dev Reprod. 2016 Jun;20(2):113-21. doi: 10.12717/DR.2016.20.2.113.

Effect of Lipopolysaccharide (LPS) Exposure on the Reproductive Organs of Immature Female Rats.

Development & reproduction

Da Kyung Yoo, Sung-Ho Lee

Affiliations

  1. Dept. of Life Science, Sangmyung University, Seoul 03016, Korea.

PMID: 27660826 PMCID: PMC5027216 DOI: 10.12717/DR.2016.20.2.113

Abstract

Lipopolysaccharide (LPS), an endotoxin, elicits strong immune responses in mammals. Several lines of evidence demonstrate that LPS challenge profoundly affects female reproductive function. For example, LPS exposure affects steroidogenesis and folliculogenesis, resulting in delayed puberty onset. The present study was conducted to clarify the mechanism underlying the adverse effect of LPS on the delayed puberty in female rats. LPS was daily injected for 5 days (50 μg/kg, PND 25-29) to treated animals and the date at VO was evaluated through daily visual examination. At PND 39, animals were sacrificed, and the tissues were immediately removed and weighed. Among the reproductive organs, the weights of the ovaries and oviduct from LPS-treated animals were significantly lower than those of control animals. There were no changes in the weights of uterus and vagina between the LPS-treated and their control animals. Immunological challenge by LPS delayed VO. Multiple corpora lutea were found in the control ovaries, indicating ovulations were occurred. However, none of corpus luteum was present in the LPS-treated ovary. The transcription level of steroidogenic acute regulatory protein (StAR), CYP11A1, CYP17A1 and CYP19 were significantly increased by LPS treatment. On the other hand, the levels of 3β- HSD, 17β-HSD and LH receptor were not changed by LPS challenge. In conclusion, the present study demonstrated that the repeated LPS exposure during the prepubertal period could induce multiple alterations in the steroidogenic machinery in ovary, and in turn, delayed puberty onset. The prepubertal LPS challenge model used in our study is useful to understand the reciprocal regulation of immune (stress) - reproductive function in early life.

Keywords: Delayed puberty onset; Lipopolysaccharide (LPS); Ovary; Rat; Steroidogenesis

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