Vasc Specialist Int. 2016 Sep;32(3):105-112. doi: 10.5758/vsi.2016.32.3.105. Epub 2016 Sep 30.
Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population.
Vascular specialist international
Khalil Mahmoodi, Leila Nasehi, Elham Karami, Mohammad Soleiman Soltanpour
Affiliations
Affiliations
- Department of Cardiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Tehran.
- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran.
- Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.
PMID: 27699157
PMCID: PMC5045252 DOI: 10.5758/vsi.2016.32.3.105
Abstract
PURPOSE: The endothelial nitric oxide synthase (eNOS) G894T polymorphism has been reported to cause endothelial dysfunction and may have a role in the development of coronary artery disease (CAD). The aim of the present study was to investigate the association of eNOS G894T genetic polymorphism and plasma levels of nitric oxide (NO) with CAD risk in an Iranian population.
MATERIALS AND METHODS: We studied 200 patients with angiographically documented CAD and 100 matched controls. Analysis of G894T genetic polymorphism of eNOS was performed by polymerase chain reaction-restriction fragment length polymorphism method. Plasma levels of NO were determined using Griess method. Biochemical analysis was conducted by routine colorimetric methods.
RESULTS: Plasma levels of NO were significantly lower in CAD patients than control subjects (41.60±12.70 vs. 55.48±16.57, P=0.001). Also, the mean plasma levels of NO were significantly lower in T allele carriers of eNOS G894T polymorphism than G allele carriers (P<0.001). The genotype distribution and minor T allele frequency of eNOS G894T polymorphism significantly differed between CAD patients and control subjects (P<0.05). However, no significant association was found between the eNOS G894T polymorphism and the severity of CAD (number of diseased vessel) or the lipid profile of CAD patients (P>0.05).
CONCLUSION: Reduced plasma level of NO is associated with increased risk of CAD in our population. Moreover, eNOS G894T polymorphism is a significant risk factor for CAD development via reducing the plasma levels of NO. However, eNOS G894T polymorphism is not a contributing factor for the severity of CAD.
Keywords: Coronary artery disease; Nitric oxide synthase type III; Polymerase chain reaction-restriction fragment length polymorphism; Single nucleotide polymorphism G894T
References
- Clin Chem. 1972 Jun;18(6):499-502 - PubMed
- J Biol Chem. 1993 Aug 15;268(23):17478-88 - PubMed
- Methods Enzymol. 1996;268:237-46 - PubMed
- BMC Med Genet. 2008 May 21;9:43 - PubMed
- Mol Aspects Med. 2005 Feb-Apr;26(1-2):33-65 - PubMed
- Clin Chem Lab Med. 2002 May;40(5):436-40 - PubMed
- Dis Markers. 2011;31(4):215-22 - PubMed
- BMC Med Genet. 2010 Sep 20;11:133 - PubMed
- Curr Vasc Pharmacol. 2012 Jan;10(1):4-18 - PubMed
- Physiol Rev. 2007 Jan;87(1):315-424 - PubMed
- Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2832-5 - PubMed
- PLoS One. 2014 Nov 19;9(11):e113363 - PubMed
- Diabetes Metab Syndr. 2012 Apr-Jun;6(2):106-9 - PubMed
- Eur Heart J Acute Cardiovasc Care. 2012 Apr;1(1):60-74 - PubMed
- Clin Chem. 2006 Jan;52(1):53-8 - PubMed
- Hypertension. 2000 Nov;36(5):885-9 - PubMed
- Biochem Genet. 2013 Feb;51(1-2):92-100 - PubMed
- Hypertension. 2001 Dec 1;38(6):1289-93 - PubMed
- PLoS One. 2014 Jan 30;9(1):e87196 - PubMed
- Exp Ther Med. 2016 May;11(5):1913-1917 - PubMed
- Circulation. 1999 Jun 22;99(24):3096-8 - PubMed
- Exp Mol Pathol. 2010 Dec;89(3):205-8 - PubMed
- Am J Physiol Heart Circ Physiol. 2001 Nov;281(5):H1908-12 - PubMed
- Circ Res. 2014 Jun 6;114(12):1852-66 - PubMed
- Mol Biol Rep. 2010 Jan;37(1):171-8 - PubMed
- Genet Med. 2007 Jun;9(6):325-31 - PubMed
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