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Reine TM, Jenssen TG, Kolset SO. Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro. Food Nutr Res. 2016;60:32615doi: 10.3402/fnr.v60.32615.
Reine, T. M., Jenssen, T. G., & Kolset, S. O. (2016). Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro. Food & nutrition research, 6032615. https://doi.org/10.3402/fnr.v60.32615
Reine, Trine M, et al. "Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro." Food & nutrition research vol. 60 (2016): 32615. doi: https://doi.org/10.3402/fnr.v60.32615
Reine TM, Jenssen TG, Kolset SO. Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro. Food Nutr Res. 2016 Sep 23;60:32615. doi: 10.3402/fnr.v60.32615. eCollection 2016. PMID: 27667774; PMCID: PMC5035772.
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Food Nutr Res. 2016 Sep 23;60:32615. doi: 10.3402/fnr.v60.32615. eCollection 2016.

Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro.

Food & nutrition research

Trine M Reine, Trond Geir Jenssen, Svein Olav Kolset

Affiliations

  1. Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
  2. Section of Nephrology, Department of Transplant Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway; [email protected].
  3. Section of Nephrology, Department of Transplant Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
  4. Metabolic and Renal Research Group, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway.

PMID: 27667774 PMCID: PMC5035772 DOI: 10.3402/fnr.v60.32615

Abstract

PURPOSE: Glucosamine (GlcN) supplements are promoted for medical reasons, for example, for patients with arthritis and other joint-related diseases. Oral intake of GlcN is followed by uptake in the intestine, transport in the circulation and thereafter delivery to chondrocytes. Here, it is postulated to have an effect on synthesis and turnover of extracellular matrix constituents expressed by these cells. Following uptake in the intestine, serum levels are transiently increased, and the endothelium is exposed to increased levels of GlcN. We investigated the possible effects of GlcN on synthesis of proteoglycans (PGs), an important matrix component, in primary human endothelial cells.

METHODS: Primary human endothelial cells were cultured in vitro in medium with 5 mM glucose and 0-10 mM GlcN. PGs were recovered and analysed by western blotting, or by SDS-PAGE, gel chromatography or ion-exchange chromatography of (35)S-PGs after (35)S-sulphate labelling of the cells.

RESULTS: The synthesis and secretion of (35)S-PGs from cultured endothelial cells were reduced in a dose- and time-dependent manner after exposure to GlcN. PGs are substituted with sulphated glycosaminoglycan (GAG) chains, vital for PG function. The reduction in (35)S-PGs was not related to an effect on GAG chain length, number or sulphation, but rather to the total expression of PGs.

CONCLUSION: Exposure of endothelial cells to GlcN leads to a general decrease in (35)S-PG synthesis. These results suggest that exposure to high levels of GlcN can lead to decreased matrix synthesis, contrary to what has been claimed by supporters of such supplements.

Keywords: arthritis; endothelial cells; glucosamine; glycosaminoglycan; proteoglycan

Conflict of interest statement

and funding The authors have not received any funding or benefits from industry or elsewhere to conduct this study.

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