Display options
Cite
Jaafar C, Omais S, Al Lafi S, et al. Role of Rb during Neurogenesis and Axonal Guidance in the Developing Olfactory System. Front Mol Neurosci. 2016;9:81doi: 10.3389/fnmol.2016.00081.
Jaafar, C., Omais, S., Al Lafi, S., El Jamal, N., Noubani, M., Skaf, L., & Ghanem, N. (2016). Role of Rb during Neurogenesis and Axonal Guidance in the Developing Olfactory System. Frontiers in molecular neuroscience, 981. https://doi.org/10.3389/fnmol.2016.00081
Jaafar, Carine, et al. "Role of Rb during Neurogenesis and Axonal Guidance in the Developing Olfactory System." Frontiers in molecular neuroscience vol. 9 (2016): 81. doi: https://doi.org/10.3389/fnmol.2016.00081
Jaafar C, Omais S, Al Lafi S, El Jamal N, Noubani M, Skaf L, Ghanem N. Role of Rb during Neurogenesis and Axonal Guidance in the Developing Olfactory System. Front Mol Neurosci. 2016 Sep 09;9:81. doi: 10.3389/fnmol.2016.00081. eCollection 2016. PMID: 27667971; PMCID: PMC5016521.
Copy Download .nbib Format: NLM
Share it on

Front Mol Neurosci. 2016 Sep 09;9:81. doi: 10.3389/fnmol.2016.00081. eCollection 2016.

Role of Rb during Neurogenesis and Axonal Guidance in the Developing Olfactory System.

Frontiers in molecular neuroscience

Carine Jaafar, Saad Omais, Sawsan Al Lafi, Nadim El Jamal, Mohammad Noubani, Larissa Skaf, Noël Ghanem

Affiliations

  1. Department of Biology, American University of Beirut Beirut, Lebanon.

PMID: 27667971 PMCID: PMC5016521 DOI: 10.3389/fnmol.2016.00081

Abstract

The Retinoblastoma protein, Rb, was shown to regulate distinct aspects of neurogenesis in the embryonic and adult brain besides its primary role in cell cycle control. It is still unknown, however, whether Rb is required for tissue morphogenesis and the establishment of synaptic connections between adjacent tissues during development. We have investigated here the role of Rb during development of the olfactory system (OS), which heavily relies on reciprocal interactions between the olfactory epithelium (OE) and the olfactory bulb (OB). We show that mice carrying a telencephalic-specific deletion of Rb display several neurogenic defects in the OS during late development. In the OE, loss of Rb leads to ectopic proliferation of late-born progenitors (Tuj-1+), abnormal radial migration and terminal maturation of olfactory sensory neurons (OSNs). In the OB, deletion of Rb causes severe lamination defects with loss of clear boundaries between distinct layers. Importantly, starting around E15.5 when OB glomerulogenesis is initiated, many OSNs axons that project along the olfactory nerve layer (ONL) fail to properly innervate the nascent bulb, thus resulting in partial loss of connectivity between OE-OB and gradual neuronal degeneration in both tissues peaking at birth. This deficiency correlates with deregulated expressions of two key chemo-repellant molecules, Robo2/Slit1 and Nrp2/Sema3F that control the formation of dorsal-ventral topographic map of OSNs connections with OB glomeruli. This study highlights a critical requirement for Rb during neurogenesis and the establishment of proper synaptic connections inside the OS during development.

Keywords: Rb; axonal guidance; cell survival; neurogenesis; olfactory bulb; olfactory epithelium; signaling molecules

References

  1. Stem Cell Rev. 2013 Dec;9(6):794-805 - PubMed
  2. Mol Cell Biol. 2007 Jul;27(13):4825-43 - PubMed
  3. J Comp Neurol. 2006 Jun 1;496(4):529-43 - PubMed
  4. Annu Rev Cell Dev Biol. 2006;22:713-37 - PubMed
  5. Dev Biol. 1980 Jan;74(1):205-15 - PubMed
  6. Int J Dev Neurosci. 2014 Oct;37:76-86 - PubMed
  7. J Neurosci. 1998 Nov 1;18(21):8919-27 - PubMed
  8. J Neurosci. 2004 Jul 28;24(30):6676-85 - PubMed
  9. EMBO J. 2005 Dec 21;24(24):4381-91 - PubMed
  10. Neuron. 1993 Jun;10(6):1035-48 - PubMed
  11. J Neurosci. 2012 Jun 13;32(24):8219-30 - PubMed
  12. Development. 2009 May;136(9):1453-64 - PubMed
  13. J Neurosci. 2012 Feb 29;32(9):2964-75 - PubMed
  14. J Neurosci. 2007 Aug 22;27(34):9094-104 - PubMed
  15. Stain Technol. 1983 Mar;58(2):121-2 - PubMed
  16. Cell. 2010 Jun 11;141(6):1056-67 - PubMed
  17. Semin Cell Dev Biol. 2006 Aug;17(4):462-70 - PubMed
  18. J Neurosci. 2011 May 25;31(21):7920-6 - PubMed
  19. J Comp Neurol. 1968 Nov;134(3):287-304 - PubMed
  20. Mol Cell Biol. 2011 Jan;31(2):238-47 - PubMed
  21. Cell Mol Neurobiol. 2006 Jul-Aug;26(4-6):803-21 - PubMed
  22. Genes Dev. 2000 Apr 15;14(8):994-1004 - PubMed
  23. J Neurosci. 2007 May 30;27(22):5926-35 - PubMed
  24. Neuroscience. 1992;46(3):617-29 - PubMed
  25. J Comp Neurol. 1997 Mar 10;379(2):171-84 - PubMed
  26. Genes Dev. 2012 Jul 1;26(13):1409-20 - PubMed
  27. J Comp Neurol. 1996 Feb 26;366(1):1-14 - PubMed
  28. Cell Death Differ. 2002 Dec;9(12):1279-86 - PubMed
  29. Hippocampus. 2016 Nov;26(11):1379-1392 - PubMed
  30. Brain Res. 1987 Oct 20;424(1):144-52 - PubMed
  31. Neuroscience. 1992 Jul;49(2):255-75 - PubMed
  32. Behav Brain Res. 2012 Feb 14;227(2):480-9 - PubMed
  33. J Neurosci. 2012 Oct 17;32(42):14809-14 - PubMed
  34. J Neurosci. 2005 Apr 6;25(14):3586-92 - PubMed
  35. Curr Opin Genet Dev. 2003 Oct;13(5):543-50 - PubMed
  36. Cell Cycle. 2006 Jul;5(14):1506-13 - PubMed
  37. Ann N Y Acad Sci. 2007 Dec;1121:87-101 - PubMed
  38. J Comp Neurol. 1999 Oct 18;413(2):289-304 - PubMed
  39. Glia. 2011 Feb;59(2):278-92 - PubMed
  40. Development. 2016 May 1;143(9):1534-46 - PubMed
  41. J Neurosci. 2008 Apr 16;28(16):4244-9 - PubMed
  42. Sci Rep. 2016 Feb 05;6:20230 - PubMed
  43. J Neurophysiol. 2004 Jun;91(6):2532-40 - PubMed
  44. Cold Spring Harb Perspect Biol. 2010 Sep;2(9):a001933 - PubMed
  45. J Neurosci. 2003 Jan 15;23(2):568-78 - PubMed
  46. EMBO J. 2002 Jul 1;21(13):3337-46 - PubMed
  47. J Neurocytol. 1979 Feb;8(1):1-18 - PubMed
  48. Development. 1999 Jul;126(13):2901-9 - PubMed
  49. Neuron. 2000 Nov;28(2):411-25 - PubMed
  50. Neuron. 1989 Jul;3(1):115-27 - PubMed
  51. J Comp Neurol. 2000 Dec 18;428(3):511-26 - PubMed
  52. Ann N Y Acad Sci. 1998 Nov 30;855:83-94 - PubMed
  53. Mol Cell Neurosci. 2010 Jul;44(3):233-45 - PubMed
  54. Dev Biol. 2012 Nov 15;371(2):269-79 - PubMed
  55. Dev Biol. 2000 Jun 15;222(2):296-306 - PubMed

Publication Types