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Eynali S, Khoei S, Khoee S, et al. Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29. Int J Hyperthermia. 2016;33(3):327-335doi: 10.1080/02656736.2016.1243260.
Eynali, S., Khoei, S., Khoee, S., & Esmaelbeygi, E. (2017). Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 33(3), 327-335. https://doi.org/10.1080/02656736.2016.1243260
Eynali, Samira, et al. "Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29." International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group vol. 33,3 (2017): 327-335. doi: https://doi.org/10.1080/02656736.2016.1243260
Eynali S, Khoei S, Khoee S, Esmaelbeygi E. Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29. Int J Hyperthermia. 2017 May;33(3):327-335. doi: 10.1080/02656736.2016.1243260. Epub 2016 Oct 23. PMID: 27701929.
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Int J Hyperthermia. 2017 May;33(3):327-335. doi: 10.1080/02656736.2016.1243260. Epub 2016 Oct 23.

Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group

Samira Eynali, Samideh Khoei, Sepideh Khoee, Elaheh Esmaelbeygi

Affiliations

  1. a Department of Medical Physics and Biomedical Engineering, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
  2. b Department of Medical Physics, School of Medicine , Iran University of Medical Sciences , Tehran , Iran.
  3. c Razi Drug Research Centre, Iran University of Medical Sciences , Tehran , Iran.
  4. d Department of Polymer Chemistry, School of Sciences , University of Tehran , Tehran , Iran.

PMID: 27701929 DOI: 10.1080/02656736.2016.1243260

Abstract

The purpose of this study was to evaluate the combined effects of heat and polylactic-co-glycolic acid (PLGA) nanoparticles, as 5-fluorouracil carriers with/without iron oxide core, on the viability and proliferation capacity of human colon cancer cell line HT-29 in the spheroid model. HT-29 spheroid cells were treated with different concentrations of 5-FU or 5-FU-loaded into both nanoparticles for 74 h. Hyperthermia was then performed at 43 °C for 60 min. Finally, the effects of the mentioned treatments on cell viability and proliferation capacity were evaluated using the trypan blue dye exclusion test and colony formation assay, respectively. Our results showed that hyperthermia, in combination with 5-FU or PLGA nanoparticles as 5-FU carriers, significantly enhanced the cytotoxic effects as compared to the control group. Considering that nanoparticles could increase the intracellular concentration of drugs in cancer cells, the extent of cytotoxic effects following treatment with 5-FU-loaded into both nanoparticles was significantly higher than that with free 5-FU. In addition, the presence of iron oxide cores in nanoparticles during hyperthermia enhanced the cytotoxic effects of hyperthermia compared with nanoparticles without iron oxide core. Based on this study, hyperthermia in combination with 5-FU-loaded PLGA nanoparticles with iron oxide core drastically reduced the proliferation capacity of HT-29 cells; therefore, it may be considered as a new direction in the treatment of colon cancer.

Keywords: 5-fluorouracil; Hyperthermia; PLGA nanoparticles with/without iron oxide core; colon cancer; colony formation assay

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