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Padermshoke A, Ogawa T, Nishio K, et al. Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena. PLoS One. 2016;11(9):e0162827doi: 10.1371/journal.pone.0162827.
Padermshoke, A., Ogawa, T., Nishio, K., Nakazawa, M., Nakamoto, M., Okazawa, A., Kanaya, S., Arita, M., & Ohta, D. (2016). Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena. PloS one, 11(9), e0162827. https://doi.org/10.1371/journal.pone.0162827
Padermshoke, Adchara, et al. "Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena." PloS one vol. 11,9 (2016): e0162827. doi: https://doi.org/10.1371/journal.pone.0162827
Padermshoke A, Ogawa T, Nishio K, Nakazawa M, Nakamoto M, Okazawa A, Kanaya S, Arita M, Ohta D. Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena. PLoS One. 2016 Sep 26;11(9):e0162827. doi: 10.1371/journal.pone.0162827. eCollection 2016. PMID: 27669566; PMCID: PMC5036851.
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PLoS One. 2016 Sep 26;11(9):e0162827. doi: 10.1371/journal.pone.0162827. eCollection 2016.

Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena.

PloS one

Adchara Padermshoke, Takumi Ogawa, Kazuki Nishio, Masami Nakazawa, Masatoshi Nakamoto, Atsushi Okazawa, Shigehiko Kanaya, Masanori Arita, Daisaku Ohta

Affiliations

  1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
  2. Graduate School of Information Science, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
  3. Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka, Japan.
  4. RIKEN Center for Sustainable Resource Science, Tsurumi, Kanagawa, Japan.

PMID: 27669566 PMCID: PMC5036851 DOI: 10.1371/journal.pone.0162827

Abstract

Accumulation profiles of wax esters in Euglena gracilis Z were studied under several environmental conditions. The highest amount of total wax esters accumulated under hypoxia in the dark, and C28 (myristyl-myristate, C14:0-C14:0) was prevalent among all conditions investigated. The wax ester production was almost completely suppressed under anoxia in the light, and supplying exogenous inorganic carbon sources restored wax ester fermentation, indicating the need for external carbon sources for the wax ester fermentation. 13C-labeling experiments revealed specific isotopic enrichment in the odd-numbered fatty acids derived from wax esters, indicating that the exogenously-supplied CO2 was incorporated into wax esters via the propionyl-CoA pathway through the reverse tricarboxylic acid (TCA) cycle. The addition of 3-mercaptopicolinic acid, a phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, significantly affected the incorporation of 13C into citrate and malate as the biosynthetic intermediates of the odd-numbered fatty acids, suggesting the involvement of PEPCK reaction to drive wax ester fermentation. Additionally, the 13C-enrichment pattern of succinate suggested that the CO2 assimilation might proceed through alternative pathways in addition to the PEPCK reaction. The current results indicate that the mechanisms of anoxic CO2 assimilation are an important target to reinforce wax ester fermentation in Euglena.

Conflict of interest statement

The authors have declared that no competing interests exist.

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