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Becker A, Klapczynski A, Kuch N, et al. Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light. Sci Rep. 2016;6:33847doi: 10.1038/srep33847.
Becker, A., Klapczynski, A., Kuch, N., Arpino, F., Simon-Keller, K., De La Torre, C., Sticht, C., van Abeelen, F. A., Oversluizen, G., & Gretz, N. (2016). Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light. Scientific reports, 633847. https://doi.org/10.1038/srep33847
Becker, Anja, et al. "Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light." Scientific reports vol. 6 (2016): 33847. doi: https://doi.org/10.1038/srep33847
Becker A, Klapczynski A, Kuch N, Arpino F, Simon-Keller K, De La Torre C, Sticht C, van Abeelen FA, Oversluizen G, Gretz N. Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light. Sci Rep. 2016 Sep 27;6:33847. doi: 10.1038/srep33847. PMID: 27669902; PMCID: PMC5037386.
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Sci Rep. 2016 Sep 27;6:33847. doi: 10.1038/srep33847.

Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light.

Scientific reports

Anja Becker, Anna Klapczynski, Natalia Kuch, Fabiola Arpino, Katja Simon-Keller, Carolina De La Torre, Carsten Sticht, Frank A van Abeelen, Gerrit Oversluizen, Norbert Gretz

Affiliations

  1. Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
  2. Philips Group Innovation, Research, High Tech Campus 34, 7.031, 5656 AE Eindhoven, The Netherlands.

PMID: 27669902 PMCID: PMC5037386 DOI: 10.1038/srep33847

Abstract

Photobiomodulation (PBM) with blue light induces a biphasic dose response curve in proliferation of immortalized human keratinocytes (HaCaT), with a maximum anti-proliferative effect reached with 30min (41.4 J/cm2). The aim of this study was to test the photobiomodulatory effect of 41.4 J/cm2 blue light irradiation on ROS production, apoptosis and gene expression at different time points after irradiation of HaCaT cells in vitro and assess its safety. ROS concentration was increased 30 min after irradiation. However, already 1 h after irradiation, cells were able to reduce ROS and balance the concentration to a normal level. The sudden increase in ROS did not damage the cells, which was demonstrated with FACS analysis where HaCaT cells did not show any sign of apoptosis after blue light irradiation. Furthermore, a time course could be seen in gene expression analysis after blue light, with an early response of stimulated genes already 1 h after blue light irradiation, leading to the discovery of the aryl hydrocarbon receptor as possible target for blue light irradiation.

References

  1. Photomed Laser Surg. 2011 May;29(5):285-304 - PubMed
  2. Proc Natl Acad Sci U S A. 2007 May 22;104(21):8851-6 - PubMed
  3. Philos Trans R Soc Lond B Biol Sci. 2013 Jan 06;368(1612):20120431 - PubMed
  4. Nat Med. 2014 Jul;20(7):709-11 - PubMed
  5. Mol Cell Biol. 2004 Dec;24(24):10941-53 - PubMed
  6. J Biol Chem. 2000 Jan 28;275(4):2943-50 - PubMed
  7. Annu Rev Pharmacol Toxicol. 2000;40:519-61 - PubMed
  8. Dose Response. 2014 Jan 31;12(2):288-341 - PubMed
  9. Drug Metab Dispos. 2007 Jul;35(7):1009-16 - PubMed
  10. Lasers Surg Med. 1985;5(1):31-9 - PubMed
  11. Drug Resist Updat. 2012 Aug;15(4):223-36 - PubMed
  12. J Invest Dermatol. 2010 Jan;130(1):259-69 - PubMed
  13. Cell Metab. 2014 May 6;19(5):757-66 - PubMed
  14. J Invest Dermatol. 2012 Nov;132(11):2505-21 - PubMed
  15. Exp Biol Med (Maywood). 2012 Nov;237(11):1241-8 - PubMed
  16. Sci Rep. 2015 Jun 01;5:10581 - PubMed
  17. Cochrane Database Syst Rev. 2011 Dec 07;(12):CD007969 - PubMed
  18. Curr Biol. 2014 May 19;24(10):R453-62 - PubMed
  19. Trends Biochem Sci. 2014 Apr;39(4):199-218 - PubMed
  20. J Periodontal Implant Sci. 2013 Dec;43(6):262-8 - PubMed
  21. J Biol Chem. 2005 Jul 22;280(29):27244-50 - PubMed
  22. Anticancer Agents Med Chem. 2014 Jan;14(1):77-96 - PubMed
  23. Toxicol Lett. 2014 Nov 18;231(1):99-107 - PubMed
  24. Br J Pharmacol. 2006 Jun;148(3):245-54 - PubMed
  25. J Biophotonics. 2012 Nov;5(11-12):827-37 - PubMed
  26. Chem Res Toxicol. 1995 Sep;8(6):847-58 - PubMed
  27. Mol Cell. 2009 Oct 9;36(1):131-40 - PubMed
  28. Drug Metab Rev. 2007;39(1):235-48 - PubMed
  29. Genes Dev. 1999 Jan 1;13(1):20-5 - PubMed
  30. Biol Chem. 2006 Sep;387(9):1149-57 - PubMed
  31. Immunity. 2014 Jun 19;40(6):863-4 - PubMed
  32. Anticancer Res. 2013 Jul;33(7):2823-31 - PubMed
  33. Immunity. 2014 Jun 19;40(6):989-1001 - PubMed
  34. J Biol Chem. 1998 Aug 28;273(35):22708-13 - PubMed
  35. Genes Dev. 1999 Jul 1;13(13):1742-53 - PubMed
  36. Mol Cell. 2012 Oct 26;48(2):158-67 - PubMed
  37. J Biol Chem. 2005 May 27;280(21):20340-8 - PubMed
  38. J Clin Laser Med Surg. 2001 Dec;19(6):305-14 - PubMed
  39. Adv Anat Embryol Cell Biol. 2012;212:v, vii, 1-115 - PubMed
  40. Biochem Soc Trans. 2015 Aug;43(4):621-6 - PubMed
  41. Annu Rev Pharmacol Toxicol. 2003;43:309-34 - PubMed
  42. Photomed Laser Surg. 2005 Aug;23(4):355-61 - PubMed
  43. Biochem Pharmacol. 2013 Sep 1;86(5):561-70 - PubMed
  44. Wound Repair Regen. 2007 Nov-Dec;15(6):866-74 - PubMed
  45. J Biol Chem. 2004 Jun 4;279(23):23847-50 - PubMed
  46. Biol Pharm Bull. 2012;35(6):818-23 - PubMed
  47. Dermatology. 2015;231(1):24-34 - PubMed
  48. J Endocrinol. 2003 Sep;178(3):347-55 - PubMed
  49. Photomed Laser Surg. 2015 Apr;33(4):183-4 - PubMed
  50. Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506 - PubMed
  51. Nucleic Acids Res. 2005 Nov 10;33(20):e175 - PubMed
  52. PLoS One. 2011;6(7):e22453 - PubMed
  53. Nat Rev Mol Cell Biol. 2014 Jun;15(6):411-21 - PubMed
  54. Redox Biol. 2015 Dec;6:183-97 - PubMed
  55. Biol Chem. 2006 Sep;387(9):1195-9 - PubMed
  56. Nat Med. 2014 Jul;20(7):711-3 - PubMed
  57. Biochem Pharmacol. 2000 Jan 1;59(1):65-85 - PubMed

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