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Ye S, Song Z, Li J, et al. Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. Int J Endocrinol. 2016;2016:6704851doi: 10.1155/2016/6704851.
Ye, S., Song, Z., Li, J., Li, C., Yang, J., & Chang, B. (2016). Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. International journal of endocrinology, 20166704851. https://doi.org/10.1155/2016/6704851
Ye, Shoujiao, et al. "Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats." International journal of endocrinology vol. 2016 (2016): 6704851. doi: https://doi.org/10.1155/2016/6704851
Ye S, Song Z, Li J, Li C, Yang J, Chang B. Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. Int J Endocrinol. 2016;2016:6704851. doi: 10.1155/2016/6704851. Epub 2016 Sep 15. PMID: 27703477; PMCID: PMC5040811.
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Int J Endocrinol. 2016;2016:6704851. doi: 10.1155/2016/6704851. Epub 2016 Sep 15.

Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats.

International journal of endocrinology

Shoujiao Ye, Zhenqiang Song, Jing Li, Chunshen Li, Juhong Yang, Bai Chang

Affiliations

  1. Endocrinology, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, No. 66, Tongan Road, Heping District, Tianjin 300070, China; Endocrinology, Nanyang TCM Hospital, No. 939, Qiyi Road, Wolong District, Nanyang, Henan 473000, China; Clinical Medicine Combined with Chinese Traditional Medicine and Western Medicine, Tianjin Chinese Medical University, No. 88, Yuquan Road, Nankai District, Tianjin 300193, China.
  2. Endocrinology, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, No. 66, Tongan Road, Heping District, Tianjin 300070, China.
  3. Clinical Medicine Combined with Chinese Traditional Medicine and Western Medicine, Tianjin Chinese Medical University, No. 88, Yuquan Road, Nankai District, Tianjin 300193, China.

PMID: 27703477 PMCID: PMC5040811 DOI: 10.1155/2016/6704851

Abstract

In the study, type 2 diabetic rat model was established using streptozotocin (STZ) combined with a high-fat diet, and the rats were divided into control and diabetic groups. Diabetic groups were further divided into nonintervening, simvastatin, Didang Decoction (DDD) early-phase intervening, DDD mid-phase intervening, and DDD late-phase intervening groups. The expression level of MLCK was detected using Western Blot analysis, and the levels of cyclic adenosine monophosphate (cAMP), protein kinase C (PKC), and protein kinase A (PKA) were examined using Real Time PCR. Under the electron microscope, the cells in the early-DDD-intervention group and the simvastatin group were significantly more continuous and compact than those in the diabetic group. Compared with the control group, the expression of cAMP-1 and PKA was decreased in all diabetic groups, whereas the expression of MLCK and PKC was increased in early- and mid-phase DDD-intervening groups (

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