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Balakrishnan S, Antony PX, Mukhopadhyay HK, et al. Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India. Vet World. 2016;9(7):705-9doi: 10.14202/vetworld.2016.705-709.
Balakrishnan, S., Antony, P. X., Mukhopadhyay, H. K., Pillai, R. M., Thanislass, J., Padmanaban, V., & Srinivas, M. V. (2016). Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India. Veterinary world, 9(7), 705-9. https://doi.org/10.14202/vetworld.2016.705-709
Balakrishnan, Sangeetha, et al. "Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India." Veterinary world vol. 9,7 (2016): 705-9. doi: https://doi.org/10.14202/vetworld.2016.705-709
Balakrishnan S, Antony PX, Mukhopadhyay HK, Pillai RM, Thanislass J, Padmanaban V, Srinivas MV. Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India. Vet World. 2016 Jul;9(7):705-9. doi: 10.14202/vetworld.2016.705-709. Epub 2016 Jul 11. PMID: 27536030; PMCID: PMC4983120.
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Vet World. 2016 Jul;9(7):705-9. doi: 10.14202/vetworld.2016.705-709. Epub 2016 Jul 11.

Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India.

Veterinary world

Sangeetha Balakrishnan, Prabhakar Xavier Antony, Hirak Kumar Mukhopadhyay, Raghavan Madhusoodanan Pillai, Jacob Thanislass, Vijayalakshmi Padmanaban, Mouttou Vivek Srinivas

Affiliations

  1. Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry - 605 009, India.
  2. Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry - 605 009, India.
  3. Department of Veterinary Medicine, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry - 605 009, India.

PMID: 27536030 PMCID: PMC4983120 DOI: 10.14202/vetworld.2016.705-709

Abstract

AIM: The present study was undertaken to characterize the mutation in gyrA (DNA gyrase) and parC (topoisomerase IV) genes responsible for fluoroquinolone resistance in Escherichia coli isolates associated with the bovine mastitis.

MATERIALS AND METHODS: A total of 92 milk samples from bovine mastitis cases were sampled in and around Puducherry (Southern India). Among these samples, 30 isolates were bacteriologically characterized as E. coli. Minimum inhibitory concentrations (MIC) of fluoroquinolones of these 30 E. coli isolates were evaluated by resazurin microtiter assay. Then, the quinolone resistance determining region (QRDR) (gyrA and parC genes) of these E. coli isolates was genetically analyzed for determining the chromosomal mutation causing fluoroquinolone resistance.

RESULTS: E. coli isolates showed a resistance rate of 63.33%, 23.33% and 30.03% to nalidixic acid, ciprofloxacin and enrofloxacin, respectively. Mutations were found at 83(rd) and 87(th) amino acid position of gyrA gene, and at 80(th) and 108(th) amino acid position of parC gene in our study isolates. Among these five isolates, one had a single mutation at 83 amino acid position of gyrA with reduced susceptibility (0.5 µg/ml) to ciprofloxacin. Then, in remaining four isolates, three isolates showed triple mutation (at gyrA: S83⟶L and D87⟶N; at parC: S80⟶I) and the fifth isolate showed an additional mutation at codon 108 of parC (A108⟶T) with the increased ciprofloxacin MIC of 16-128 µg/ml. The most common mutation noticed were at S83⟶L and D87⟶N of gyrA and S80⟶I of ParC.

CONCLUSION: The study confirms the presence of mutation/s responsible for fluoroquinolone resistance in QRDR of gyrA and parC genes of E. coli isolates of animal origin, and there is increased rate of fluoroquinolone resistance with high-level of MIC. The mutations observed in this study were similar to that of human isolates.

Keywords: Escherichia coli; fluoroquinolones; gyrA; parC; quinolone resistance determining region

References

  1. Vet Ital. 2013 Jul-Sep;49(3):291-7 - PubMed
  2. Microb Drug Resist. 2014 Apr;20(2):162-9 - PubMed
  3. Poult Sci. 2014 Apr;93(4):856-63 - PubMed
  4. Microb Drug Resist. 2014 Dec;20(6):544-9 - PubMed
  5. Int J Antimicrob Agents. 2010 Apr;35(4):387-91 - PubMed
  6. Asian Pac J Trop Med. 2014 Jun;7(6):462-7 - PubMed
  7. J Antimicrob Chemother. 2009 Sep;64 Suppl 1:i29-36 - PubMed
  8. Methods. 2007 Aug;42(4):321-4 - PubMed
  9. J Med Microbiol. 2012 Nov;61(Pt 11):1591-9 - PubMed
  10. Yakushigaku Zasshi. 2003;38(2):161-79 - PubMed
  11. BMC Infect Dis. 2012 Dec 23;12:371 - PubMed
  12. Comp Immunol Microbiol Infect Dis. 2015 Aug;41:43-8 - PubMed
  13. J Clin Microbiol. 2004 Nov;42(11):4947-55 - PubMed
  14. Antimicrob Agents Chemother. 1996 Oct;40(10):2380-6 - PubMed
  15. Kansenshogaku Zasshi. 2006 Sep;80(5):507-12 - PubMed
  16. Indian J Med Res. 2011 Aug;134:214-23 - PubMed
  17. Drug Resist Updat. 1999 Feb;2(1):38-55 - PubMed
  18. Expert Rev Anti Infect Ther. 2008 Oct;6(5):733-50 - PubMed
  19. Int J Antimicrob Agents. 2005 May;25(5):358-73 - PubMed

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