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Ivanov YD, Frantsuzov PA, Zöllner A, et al. Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System. Nanoscale Res Lett. 2010;6(1):54doi: 10.1007/s11671-010-9809-5.
Ivanov, Y. D., Frantsuzov, P. A., Zöllner, A., Medvedeva, N. V., Archakov, A. I., Reinle, W., & Bernhardt, R. (2011). Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System. Nanoscale research letters, 6(1), 54. https://doi.org/10.1007/s11671-010-9809-5
Ivanov, Y D, et al. "Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System." Nanoscale research letters vol. 6,1 (2011): 54. doi: https://doi.org/10.1007/s11671-010-9809-5
Ivanov YD, Frantsuzov PA, Zöllner A, Medvedeva NV, Archakov AI, Reinle W, Bernhardt R. Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System. Nanoscale Res Lett. 2011 Dec;6(1):54. doi: 10.1007/s11671-010-9809-5. Epub 2010 Sep 30. PMID: 27502676; PMCID: PMC3212078.
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Nanoscale Res Lett. 2011 Dec;6(1):54. doi: 10.1007/s11671-010-9809-5. Epub 2010 Sep 30.

Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System.

Nanoscale research letters

Y D Ivanov, P A Frantsuzov, A Zöllner, N V Medvedeva, A I Archakov, W Reinle, R Bernhardt

Affiliations

  1. Institute of Biomedical Chemistry RAMS, Pogodinskaya st. 10, 119121, Moscow, Russia. [email protected].
  2. Institute of Biomedical Chemistry RAMS, Pogodinskaya st. 10, 119121, Moscow, Russia.
  3. Saarland University, Saarbrücken, Germany.

PMID: 27502676 PMCID: PMC3212078 DOI: 10.1007/s11671-010-9809-5

Abstract

Atomic force microscopy (AFM) and photon correlation spectroscopy (PCS) were used for monitoring of the procedure for cytochrome CYP11A1 monomerization in solution without phospholipids. It was shown that the incubation of 100 μM CYP11A1 with 12% Emulgen 913 in 50 mM KP, pH 7.4, for 10 min at T = 22°C leads to dissociation of hemoprotein aggregates to monomers with the monomerization degree of (82 ± 4)%. Following the monomerization procedure, CYP11A1 remained functionally active. AFM was employed to detect and visualize the isolated proteins as well as complexes formed between the components of the cytochrome CYP11A1-dependent steroid hydroxylase system. Both Ad and AdR were present in solution as monomers. The typical heights of the monomeric AdR, Ad and CYP11A1 images were measured by AFM and were found to correspond to the sizes 1.6 ± 0.2 nm, 1.0 ± 0.2 nm and 1.8 ± 0.2 nm, respectively. The binary Ad/AdR and AdR/CYP11A1mon complexes with the heights 2.2 ± 0.2 nm and 2.8 ± 0.2 nm, respectively, were registered by use of AFM. The Ad/CYP11A1mon complex formation reaction was kinetically characterized based on optical biosensor data. In addition, the ternary AdR/Ad/CYP11A1 complexes with a typical height of 4 ± 1 nm were AFM registered.

Keywords: Atomic force microscopy; Complex formation; Cytochrome CYP11A1; Monomerization; Photon correlation spectroscopy

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