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Tillekeratne LG, Vidanagama D, Tippalagama R, et al. Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka. Infect Chemother. 2016;48(3):160-165doi: 10.3947/ic.2016.48.3.160.
Tillekeratne, L. G., Vidanagama, D., Tippalagama, R., Lewkebandara, R., Joyce, M., Nicholson, B. P., Nagahawatte, A., Bodinayake, C. K., De Silva, A. D., & Woods, C. W. (2016). Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka. Infection & chemotherapy, 48(3), 160-165. https://doi.org/10.3947/ic.2016.48.3.160
Tillekeratne, L Gayani, et al. "Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka." Infection & chemotherapy vol. 48,3 (2016): 160-165. doi: https://doi.org/10.3947/ic.2016.48.3.160
Tillekeratne LG, Vidanagama D, Tippalagama R, Lewkebandara R, Joyce M, Nicholson BP, Nagahawatte A, Bodinayake CK, De Silva AD, Woods CW. Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka. Infect Chemother. 2016 Sep;48(3):160-165. doi: 10.3947/ic.2016.48.3.160. PMID: 27704730; PMCID: PMC5047996.
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Infect Chemother. 2016 Sep;48(3):160-165. doi: 10.3947/ic.2016.48.3.160.

Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka.

Infection & chemotherapy

L Gayani Tillekeratne, Dhammika Vidanagama, Rashmi Tippalagama, Rashmi Lewkebandara, Maria Joyce, Bradly P Nicholson, Ajith Nagahawatte, Champica K Bodinayake, Aruna Dharshan De Silva, Christopher W Woods

Affiliations

  1. Duke Global Health Institute, Durham, NC, USA. [email protected].
  2. Teaching Hospital Karapitiya, Galle, Sri Lanka.
  3. Genetech Research Institute, Colombo, Sri Lanka.
  4. Durham Veteran Affairs Medical Center, Durham, NC, USA.
  5. Department of Microbiology, Ruhuna University Faculty of Medicine, Galle, Sri Lanka.
  6. Department of Medicine, Ruhuna University Faculty of Medicine, Galle, Sri Lanka.
  7. Duke Global Health Institute, Durham, NC, USA.

PMID: 27704730 PMCID: PMC5047996 DOI: 10.3947/ic.2016.48.3.160

Abstract

BACKGROUND: Extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-PE) are increasingly reported as pathogens in urinary tract infections (UTIs). However, in Sri Lanka, the clinical and molecular epidemiology of ESBL-PE implicated in UTIs has not been well described.

MATERIALS AND METHODS: We conducted prospective, laboratory-based surveillance from October to December 2013 at a tertiary care hospital in southern Sri Lanka and enrolled patients ≥1 year of age with clinically relevant UTIs due to ESBL-PE. Isolate identity, antimicrobial drug susceptibility, and ESBL production were determined. Presence of ß-lactamase genes, bla(SHV), bla(TEM), and bla(CTX-M), was identified by polymerase chain reaction.

RESULTS: During the study period, Enterobacteriaceae were detected in 184 urine samples, with 74 (40.2%) being ESBL producers. Among 47 patients with ESBL-PE who had medical records available, 38 (80.9%) had clinically significant UTIs. Most UTIs (63.2%) were community acquired and 34.2% were in patients with diabetes. Among 36 cultured ESBL-PE isolates, significant susceptibility (>80%) was only retained to amikacin and the carbapenems. The group 1 bla(CTX-M) gene was present in 90.0% of Escherichia coli isolates and all Klebsiella pneumoniae and Enterobacter cloacae isolates. The bla(SHV) and bla(TEM) genes were more common in K. pneumoniae (75% and 50%) and E. cloacae (50% and 50%) isolates than in E. coli (10% and 20%) isolates, respectively.

CONCLUSION: The majority of UTIs caused by ESBL-PE were acquired in the community and due to organisms carrying the group 1 CTX-M ß-lactamase. Further epidemiologic studies of infections due to ESBL-PE are urgently needed to better prevent and treat these infections in South Asia.

Keywords: Community-acquired urinary tract infections; Enterobacteriaceae; Extended-spectrum ß-lactamases; Sri Lanka

Conflict of interest statement

No conflicts of interest.

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