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Ghatole K, Patil A, Giriyappa RH, et al. Evaluation of Antibacterial Efficacy of MTA with and without Additives Like Silver Zeolite and Chlorhexidine. J Clin Diagn Res. 2016;10(6):ZC11-4doi: 10.7860/JCDR/2016/18014.7913.
Ghatole, K., Patil, A., Giriyappa, R. H., Singh, T. V., Jyotsna, S. V., & Rairam, S. (2016). Evaluation of Antibacterial Efficacy of MTA with and without Additives Like Silver Zeolite and Chlorhexidine. Journal of clinical and diagnostic research : JCDR, 10(6), ZC11-4. https://doi.org/10.7860/JCDR/2016/18014.7913
Ghatole, Kiran, et al. "Evaluation of Antibacterial Efficacy of MTA with and without Additives Like Silver Zeolite and Chlorhexidine." Journal of clinical and diagnostic research : JCDR vol. 10,6 (2016): ZC11-4. doi: https://doi.org/10.7860/JCDR/2016/18014.7913
Ghatole K, Patil A, Giriyappa RH, Singh TV, Jyotsna SV, Rairam S. Evaluation of Antibacterial Efficacy of MTA with and without Additives Like Silver Zeolite and Chlorhexidine. J Clin Diagn Res. 2016 Jun;10(6):ZC11-4. doi: 10.7860/JCDR/2016/18014.7913. Epub 2016 Jun 01. PMID: 27504400; PMCID: PMC4963760.
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J Clin Diagn Res. 2016 Jun;10(6):ZC11-4. doi: 10.7860/JCDR/2016/18014.7913. Epub 2016 Jun 01.

Evaluation of Antibacterial Efficacy of MTA with and without Additives Like Silver Zeolite and Chlorhexidine.

Journal of clinical and diagnostic research : JCDR

Kiran Ghatole, Anand Patil, Ramesh Halebathi Giriyappa, Thakur Veerandar Singh, Sistla Venkata Jyotsna, Surabhi Rairam

Affiliations

  1. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery , Vikarabad, India .
  2. Professor and Head, Department of Conservative Dentistry and Endodontics, K.L.E. V.K. Institute of Dental Sciences , Belgaum, India .
  3. Professor, Department of Conservative Dentistry and Endodontics, K.L.E. V.K. Institute of Dental Sciences , Belgaum, India .
  4. Reader, Department of Conservative Dentistry and Endodontics, HKE'S S. Nijalingappa Dental College , Kalaburgi, India .

PMID: 27504400 PMCID: PMC4963760 DOI: 10.7860/JCDR/2016/18014.7913

Abstract

INTRODUCTION: Microorganisms, predominantly Enterococcus faecalis are found responsible in the progression of pulpal, periradicular diseases and in endodontic failures. Unsuccessful conventional treatment might necessitate the need for a surgical approach; where in retrograde restorative materials are used to seal the apex. Among the root end filling materials, Mineral Trioxide Aggregate (MTA) is considered biocompatible and is most commonly used in clinical applications but it has limited antibacterial activity. Metallic silver and chlorhexidine have been added into various dental materials to enhance the antibacterial activity.

AIM: This study aimed to compare the antibacterial effect of MTA, MTA mixed with silver zeolite and MTA mixed with chlorhexidine against Enterococcus faecalis.

MATERIALS AND METHODS: Test materials used in the study were divided into three groups namely Group 1- MTA, Group 2-MTA + Silver Zeolite, Group 3-MTA + Chlorhexidine. Direct contact test was done by placing a standardized suspension of Enterococcus faecalis on the test materials in a 96 well microtiter plate. The bacterial growth was measured spectrophotometrically using ELISA reader at intervals of one, three and seven days.

STATISTICAL ANALYSIS: Data was collected by recording the optical density and analyzed using two-way ANOVA and Tukey's multiple post hoc test followed by paired-t test.

RESULTS AND CONCLUSION: All test groups showed antibacterial activity against Enterococcus faecalis at day one, three and seven. MTA with silver zeolite showed the maximum antibacterial activity followed by MTA with 2% chlorhexidine. The least antibacterial effect was shown by MTA mixed with sterile water.

Keywords: Antibacterial additives; Direct contact test; Enterococcus faecalis; Root end filling materials

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