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Singer E, Andreopoulos B, Bowers RM, et al. Next generation sequencing data of a defined microbial mock community. Sci Data. 2016;3:160081doi: 10.1038/sdata.2016.81.
Singer, E., Andreopoulos, B., Bowers, R. M., Lee, J., Deshpande, S., Chiniquy, J., Ciobanu, D., Klenk, H. P., Zane, M., Daum, C., Clum, A., Cheng, J. F., Copeland, A., & Woyke, T. (2016). Next generation sequencing data of a defined microbial mock community. Scientific data, 3160081. https://doi.org/10.1038/sdata.2016.81
Singer, Esther, et al. "Next generation sequencing data of a defined microbial mock community." Scientific data vol. 3 (2016): 160081. doi: https://doi.org/10.1038/sdata.2016.81
Singer E, Andreopoulos B, Bowers RM, Lee J, Deshpande S, Chiniquy J, Ciobanu D, Klenk HP, Zane M, Daum C, Clum A, Cheng JF, Copeland A, Woyke T. Next generation sequencing data of a defined microbial mock community. Sci Data. 2016 Sep 27;3:160081. doi: 10.1038/sdata.2016.81. PMID: 27673566; PMCID: PMC5037974.
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Sci Data. 2016 Sep 27;3:160081. doi: 10.1038/sdata.2016.81.

Next generation sequencing data of a defined microbial mock community.

Scientific data

Esther Singer, Bill Andreopoulos, Robert M Bowers, Janey Lee, Shweta Deshpande, Jennifer Chiniquy, Doina Ciobanu, Hans-Peter Klenk, Matthew Zane, Christopher Daum, Alicia Clum, Jan-Fang Cheng, Alex Copeland, Tanja Woyke

Affiliations

  1. DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
  2. Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.

PMID: 27673566 PMCID: PMC5037974 DOI: 10.1038/sdata.2016.81

Abstract

Generating sequence data of a defined community composed of organisms with complete reference genomes is indispensable for the benchmarking of new genome sequence analysis methods, including assembly and binning tools. Moreover the validation of new sequencing library protocols and platforms to assess critical components such as sequencing errors and biases relies on such datasets. We here report the next generation metagenomic sequence data of a defined mock community (Mock Bacteria ARchaea Community; MBARC-26), composed of 23 bacterial and 3 archaeal strains with finished genomes. These strains span 10 phyla and 14 classes, a range of GC contents, genome sizes, repeat content and encompass a diverse abundance profile. Short read Illumina and long-read PacBio SMRT sequences of this mock community are described. These data represent a valuable resource for the scientific community, enabling extensive benchmarking and comparative evaluation of bioinformatics tools without the need to simulate data. As such, these data can aid in improving our current sequence data analysis toolkit and spur interest in the development of new tools.

Conflict of interest statement

The authors declare no competing financial interests.

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