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Kang DK, Zhao LY, Wang HL. Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano. [Cytotoxic effects of local anesthesia through lidocaine/ropivacaine on human melanoma cell lines]. Rev Bras Anestesiol. 2016;66(6):594-602doi: 10.1016/j.bjan.2016.08.002.
Kang, D. K., Zhao, L. Y., & Wang, H. L. (2016). Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano. [Cytotoxic effects of local anesthesia through lidocaine/ropivacaine on human melanoma cell lines]. Revista brasileira de anestesiologia, 66(6), 594-602. https://doi.org/10.1016/j.bjan.2016.08.002
Kang, Ding-Kun, et al. "Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano." [Cytotoxic effects of local anesthesia through lidocaine/ropivacaine on human melanoma cell lines]. Revista brasileira de anestesiologia vol. 66,6 (2016): 594-602. doi: https://doi.org/10.1016/j.bjan.2016.08.002
Kang DK, Zhao LY, Wang HL. Efeitos citotóxicos de anestesia local com lidocaína/ropivacaína em linhagens celulares de melanoma humano. [Cytotoxic effects of local anesthesia through lidocaine/ropivacaine on human melanoma cell lines]. Rev Bras Anestesiol. 2016 Nov - Dec;66(6):594-602. doi: 10.1016/j.bjan.2016.08.002. Epub 2016 Sep 14. Portuguese. PMID: 27639508.
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Rev Bras Anestesiol. 2016 Nov - Dec;66(6):594-602. doi: 10.1016/j.bjan.2016.08.002. Epub 2016 Sep 14.

[Cytotoxic effects of local anesthesia through lidocaine/ropivacaine on human melanoma cell lines].

Revista brasileira de anestesiologia

[Article in Portuguese]
Ding-Kun Kang, Li-Yan Zhao, Hong-Li Wang

Affiliations

  1. Luoyang Orthopedic - Traumatological Hospital, Department of Anesthesiology, Luoyang, China. Electronic address: [email protected].
  2. Luoyang Orthopedic - Traumatological Hospital, Department of Anesthesiology, Luoyang, China.

PMID: 27639508 DOI: 10.1016/j.bjan.2016.08.002

Abstract

BACKGROUND: Local anesthetics (LAs) are generally considered as safe, but cytotoxicity has been reported for several local anesthetics used in humans, which is not well investigated. In the present study, the cytotoxicity of lidocaine, ropivacaine and the combination of lidocaine and ropivacaine were evaluated on human melanoma cell lines. Melphalan, a nitrogen mustard alkylating agent, was used as a control agent for comparison of cytotoxic activity.

METHODS: Melanoma cell lines, A375 and Hs294T, were exposed to 1h to different concentrations of above agents. Cell-viability after exposure was determined by flow cytometry.

RESULTS: Investigated LAs showed detrimental cytotoxicity on studied melanoma cell lines in time- (p<0.001), concentration- (p<0.001), and agent dependant. In both A375 and Hs294T cell lines, minimum cell viability rates were found after 72h of exposure to these agents. Lidocaine 2% caused a reduction of vital cells to 10%±2% and 14%±2% in A375 and Hs294T, respectively after 72h of exposure. Ropivacaine 0.75% after 72h reduced viable cells to 15%±3% and 25%±3% in A375 and Hs294T, respectively. Minimum cell viability after 72h exposure to the combination was 10%±2% and 18%±2% in A375 and Hs294T, respectively. Minimum cell viability after 72h exposure to melphalan was 8%±1% and 12%±2%, in A375 and Hs294T, respectively.

CONCLUSION: LAs have cytotoxic activity on human melanoma cell lines in a time-, concentration- and agent-dependant manner. Apoptosis in the cell lines was mediated through activity of caspases-3 and caspases-8.

Copyright © 2016 Sociedade Brasileira de Anestesiologia. Publicado por Elsevier Editora Ltda. All rights reserved.

Keywords: Aminoamide local anesthetics; Anestésicos locais do grupo amino‐amida; Citometria de fluxo; Citotoxicidade; Cytotoxicity; Flow cytometry; Lidocaine; Lidocaína; Linhagens celulares de melanoma; Melanoma cell lines; Ropivacaine; Ropivacaína

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