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Hatayama Y, Hashimoto Y, Hara A, et al. Construction and Evaluation of Cytomegalovirus DNA Quantification System with Real-Time Detection Polymerase Chain Reaction. Yonago Acta Med. 2016;59(3):217-222
Hatayama, Y., Hashimoto, Y., Hara, A., & Motokura, T. (2016). Construction and Evaluation of Cytomegalovirus DNA Quantification System with Real-Time Detection Polymerase Chain Reaction. Yonago acta medica, 59(3), 217-222.
Hatayama, Yuki, et al. "Construction and Evaluation of Cytomegalovirus DNA Quantification System with Real-Time Detection Polymerase Chain Reaction." Yonago acta medica vol. 59,3 (2016): 217-222.
Hatayama Y, Hashimoto Y, Hara A, Motokura T. Construction and Evaluation of Cytomegalovirus DNA Quantification System with Real-Time Detection Polymerase Chain Reaction. Yonago Acta Med. 2016 Sep 12;59(3):217-222. eCollection 2016 Sep. PMID: 27708537; PMCID: PMC5050271.
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Yonago Acta Med. 2016 Sep 12;59(3):217-222. eCollection 2016 Sep.

Construction and Evaluation of Cytomegalovirus DNA Quantification System with Real-Time Detection Polymerase Chain Reaction.

Yonago acta medica

Yuki Hatayama, Yuki Hashimoto, Ayako Hara, Toru Motokura

Affiliations

  1. Division of Clinical Laboratory, Tottori University Hospital, Yonago 683-8504, Japan.
  2. Division of Clinical Laboratory, Tottori University Hospital, Yonago 683-8504, Japan; †Division of Clinical Laboratory Medicine, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.

PMID: 27708537 PMCID: PMC5050271

Abstract

BACKGROUND: For patients with reactivation of human cytomegalovirus (CMV), a highly sensitive and accurate CMV quantification system is essential to monitor viral load.

METHODS: We constructed a real-time detection PCR (RTD-PCR) system for CMV DNA and evaluated its linearity, lower detection limit, dynamic range and accuracy using two CMV standards. We used 219 clinical samples derived from 101 patients to compare the system with the pp65 antigen test.

RESULTS: The 95% detection limit was determined to be 556 IU/mL (95% CI, 440-797 IU/mL), and the quantification range was between 10

CONCLUSION: We constructed the RTD-PCR system which enables accurate evaluation of CMV reactivation by monitoring of viral load in immunosuppressed or immunocompromised patients.

Keywords: CMV antigenemia; RTD-PCR; cytomegalovirus; quantification

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