Display options
Cite
Valero-Gracia A, Marino R, Crocetta F, et al. Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny. Front Zool. 2016;13:45doi: 10.1186/s12983-016-0177-6.
Valero-Gracia, A., Marino, R., Crocetta, F., Nittoli, V., Tiozzo, S., & Sordino, P. (2016). Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny. Frontiers in zoology, 1345. https://doi.org/10.1186/s12983-016-0177-6
Valero-Gracia, Alberto, et al. "Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny." Frontiers in zoology vol. 13 (2016): 45. doi: https://doi.org/10.1186/s12983-016-0177-6
Valero-Gracia A, Marino R, Crocetta F, Nittoli V, Tiozzo S, Sordino P. Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny. Front Zool. 2016 Sep 29;13:45. doi: 10.1186/s12983-016-0177-6. eCollection 2016. PMID: 27708681; PMCID: PMC5041399.
Copy Download .nbib Format: NLM
Share it on

Front Zool. 2016 Sep 29;13:45. doi: 10.1186/s12983-016-0177-6. eCollection 2016.

Comparative localization of serotonin-like immunoreactive cells in Thaliacea informs tunicate phylogeny.

Frontiers in zoology

Alberto Valero-Gracia, Rita Marino, Fabio Crocetta, Valeria Nittoli, Stefano Tiozzo, Paolo Sordino

Affiliations

  1. Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy.
  2. Institute of Marine Biological Resources and Inland Waters, Hellenic Centre for Marine Research, GR-19013 Anavyssos, Greece.
  3. Observatoire Océanographique, CNRS, Sorbonne Universités, UPMC Univ Paris 06, Laboratoire de Biologie du Développement de Villefranche-sur-mer, 06230 Villefranche-sur-Mer, France.

PMID: 27708681 PMCID: PMC5041399 DOI: 10.1186/s12983-016-0177-6

Abstract

BACKGROUND: Thaliaceans is one of the understudied classes of the phylum Tunicata. In particular, their phylogenetic relationships remain an issue of debate. The overall pattern of serotonin (5-HT) distribution is an excellent biochemical trait to interpret internal relationships at order level. In the experiments reported here we compared serotonin-like immunoreactivity at different life cycle stages of two salpid, one doliolid, and one pyrosomatid species. This multi-species comparison provides new neuroanatomical data for better resolving the phylogeny of the class Thaliacea.

RESULTS: Adults of all four examined thaliacean species exhibited serotonin-like immunoreactivity in neuronal and non-neuronal cell types, whose anatomical position with respect to the nervous system is consistently identifiable due to α-tubulin immunoreactivity. The results indicate an extensive pattern that is consistent with the presence of serotonin in cell bodies of variable morphology and position, with some variation within and among orders. Serotonin-like immunoreactivity was not found in immature forms such as blastozooids (Salpida), tadpole larvae (Doliolida) and young zooids (Pyrosomatida).

CONCLUSIONS: Comparative anatomy of serotonin-like immunoreactivity in all three thaliacean clades has not been reported previously. These results are discussed with regard to studies of serotonin-like immunoreactivity in adult ascidians. Lack of serotonin-like immunoreactivity in the endostyle of Salpida and Doliolida compared to

Keywords: Comparative neuroanatomy; Evolution; Immunohistochemistry; Thaliaceans; Tunicata; Zooplankton

References

  1. Mech Dev. 1999 Sep;87(1-2):77-91 - PubMed
  2. Eur J Histochem. 2012 Apr 20;56(2):e16 - PubMed
  3. J Comp Neurol. 1999 Sep 27;412(3):527-41 - PubMed
  4. Mol Phylogenet Evol. 2002 Dec;25(3):408-28 - PubMed
  5. Teratology. 1993 Jun;47(6):573-84 - PubMed
  6. Nature. 2012 Mar 14;483(7389):289-94 - PubMed
  7. Evol Dev. 2005 Sep-Oct;7(5):483-9 - PubMed
  8. Mech Dev. 1994 Jan;45(1):31-47 - PubMed
  9. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7182-6 - PubMed
  10. Cell Tissue Res. 1988 Jul;253(1):137-43 - PubMed
  11. Dev Dyn. 2009 Aug;238(8):2081-94 - PubMed
  12. Brain Res. 1985 Jan;349(1-2):209-24 - PubMed
  13. Proc Biol Sci. 2000 Jun 7;267(1448):1071-9 - PubMed
  14. J Comp Neurol. 2013 Aug 15;521(12):2756-71 - PubMed
  15. Genome Biol Evol. 2012;4(8):740-9 - PubMed
  16. Integr Comp Biol. 2010 Jul;50(1):35-52 - PubMed
  17. Q Rev Biol. 2015 Jun;90(2):117-45 - PubMed
  18. Dev Biol. 2007 Nov 1;311(1):264-75 - PubMed
  19. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4469-74 - PubMed
  20. Cell Tissue Res. 1982;226(1):231-5 - PubMed
  21. Cell Tissue Res. 2001 Aug;305(2):177-86 - PubMed
  22. BMC Evol Biol. 2009 Aug 05;9:187 - PubMed
  23. Philos Trans R Soc Lond B Biol Sci. 2016 Jan 5;371(1685):20150050 - PubMed
  24. Front Zool. 2010 Nov 09;7:29 - PubMed
  25. Genesis. 2008 Nov;46(11):592-604 - PubMed
  26. Cell Tissue Res. 1982;223(2):369-402 - PubMed
  27. Brain Res Bull. 1983 Apr;10(4):459-79 - PubMed
  28. Nature. 2006 Feb 23;439(7079):965-8 - PubMed
  29. Placenta. 1985 Jan-Feb;6(1):53-63 - PubMed
  30. Neurochem Res. 2005 Jun-Jul;30(6-7):825-37 - PubMed
  31. J Comp Neurol. 1985 Jun 22;236(4):443-53 - PubMed
  32. BMC Evol Biol. 2012 Jul 24;12:121 - PubMed
  33. Development. 1998 Mar;125(6):1113-22 - PubMed
  34. Int Rev Neurobiol. 2007;77:31-56 - PubMed
  35. Dev Neurosci. 2005;27(6):349-63 - PubMed
  36. BMC Evol Biol. 2013 Oct 24;13:230 - PubMed
  37. J Morphol. 2013 Mar;274(3):258-74 - PubMed
  38. J Exp Biol. 2004 Mar;207 (Pt 8):1415-29 - PubMed
  39. Curr Opin Genet Dev. 2000 Aug;10(4):434-42 - PubMed
  40. Dev Growth Differ. 2001 Dec;43(6):647-56 - PubMed
  41. Int J Dev Neurosci. 1986;4(5):465-75 - PubMed
  42. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1801-4 - PubMed
  43. Syst Biol. 2000 Mar;49(1):52-64 - PubMed
  44. Curr Biol. 2005 May 10;15(9):794-803 - PubMed
  45. Development. 1997 May;124(9):1745-55 - PubMed
  46. Dev Neurobiol. 2007 May;67(6):752-63 - PubMed

Publication Types