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Rizek CF, Luiz AM, Assis GR, et al. COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS. Rev Inst Med Trop Sao Paulo. 2016;58:60doi: 10.1590/S1678-9946201658060.
Rizek, C. F., Luiz, A. M., Assis, G. R., Costa, S. F., Levin, A. S., & Lopes, M. H. (2016). COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS. Revista do Instituto de Medicina Tropical de Sao Paulo, 5860. https://doi.org/10.1590/S1678-9946201658060
Rizek, Camila F, et al. "COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS." Revista do Instituto de Medicina Tropical de Sao Paulo vol. 58 (2016): 60. doi: https://doi.org/10.1590/S1678-9946201658060
Rizek CF, Luiz AM, Assis GR, Costa SF, Levin AS, Lopes MH. COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS. Rev Inst Med Trop Sao Paulo. 2016 Sep 22;58:60. doi: 10.1590/S1678-9946201658060. PMID: 27680165; PMCID: PMC5048631.
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Rev Inst Med Trop Sao Paulo. 2016 Sep 22;58:60. doi: 10.1590/S1678-9946201658060.

COMPARISON OF METHODS TO IDENTIFY Neisseria meningitidis IN ASYMPTOMATIC CARRIERS.

Revista do Instituto de Medicina Tropical de Sao Paulo

Camila F Rizek, André Machado Luiz, Gracilene Ramos de Assis, Silvia Figueiredo Costa, Anna Sara Levin, Marta Heloisa Lopes

Affiliations

  1. University of São Paulo, School of Medicine, Department of Infectious Diseases, Laboratory of Bacteriology (LIM-54). São Paulo, SP, Brazil. E-mails: [email protected]; [email protected]; [email protected]; [email protected].
  2. University of São Paulo, School of Medicine, Department of Infectious Diseases, São Paulo, SP, Brazil. E-mails: [email protected]; [email protected].

PMID: 27680165 PMCID: PMC5048631 DOI: 10.1590/S1678-9946201658060

Abstract

Neisseria meningitidis is a cause of several life-threatening diseases and can be a normal commensal in the upper respiratory tract of healthy carriers. The carrier rate is not well established especially because there is no standard method for the isolation of N. meningitidis. Therefore, the aim of this study was to compare identification methods for the carrier state. Two swabs were collected from 190 volunteers: one was cultured and the other had DNA extracted directly from the sample. The Polymerase Chain Reaction (PCR) was performed to determine species and serogroups and compared the results between the methods. PCR for species determination used two pairs of primers and when there was only one amplicon, it was sequenced. The culture technique was positive in 23 (12.1%) subjects while the direct extraction method was positive in 132 (69.5%), p < 0.001. Among the 135 subjects with positive N. meningitides tests, 88 (65.2%) were serogroup C; 3 (2.2%) serogroup B; 5 (3.7%) were positive for both serogroup B and C, and 39 (28.9%) did not belong to any of the tested serogroups. In this study, PCR from DNA extracted directly from swabs identified more N. meningitidis asymptomatic carriers than the culture technique.

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