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Degu A, Engidawork E, Shibeshi W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC Complement Altern Med. 2016;16(1):379doi: 10.1186/s12906-016-1357-9.
Degu, A., Engidawork, E., & Shibeshi, W. (2016). Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC complementary and alternative medicine, 16(1), 379. https://doi.org/10.1186/s12906-016-1357-9
Degu, Amsalu, et al. "Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model." BMC complementary and alternative medicine vol. 16,1 (2016): 379. doi: https://doi.org/10.1186/s12906-016-1357-9
Degu A, Engidawork E, Shibeshi W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC Complement Altern Med. 2016 Sep 29;16(1):379. doi: 10.1186/s12906-016-1357-9. PMID: 27681095; PMCID: PMC5041311.
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BMC Complement Altern Med. 2016 Sep 29;16(1):379. doi: 10.1186/s12906-016-1357-9.

Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model.

BMC complementary and alternative medicine

Amsalu Degu, Ephrem Engidawork, Workineh Shibeshi

Affiliations

  1. Department of Pharmacy, College of Medicine and Health Sciences, Ambo University, P.O, Box 19, Ambo, Ethiopia. [email protected].
  2. Department of Pharmacology and Clinical Pharmacy, College of Health Sciences, School of Pharmacy, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.

PMID: 27681095 PMCID: PMC5041311 DOI: 10.1186/s12906-016-1357-9

Abstract

BACKGROUND: Traditional healers in Ethiopia use a wide range of medicinal plants with antidiarrheal properties. Among these, Croton macrostachyus is one such plant claimed to have an antidiarrheal activity in Ethiopian folklore medicine. Previous studies showed that the crude extract is endowed with the claimed property. The present study was undertaken to further the claim by screening different fractions for the said activity so that it could serve as a basis for subsequent studies.

METHODS: The fractions were obtained by successive extraction in soxhlet apparatus with solvents of different polarity (chloroform & methanol) followed by cold maceration of the deposit of the methanol fraction with distilled water. The antidiarrheal activity was evaluated using castor oil induced diarrheal model, charcoal meal test and anti-enteropooling test in mice. The test groups received various doses (300, 400, 500 mg/kg and an additional dose of 1000 mg/kg for the aqueous fraction) of the fractions, whereas positive controls received either Loperamide (3 mg/kg) or Atropine (5 mg/kg) and negative controls received vehicle (10 ml/kg).

RESULTS: In the castor oil induced model, the chloroform (at all test doses) and methanol (at 400 & 500 mg/kg) fractions significantly delayed diarrheal onset, decreased stool frequency and weight of feces. The aqueous fraction was however devoid of significant effect at all the tested doses. Chloroform and methanol fractions produced a significant dose dependent decline in the weight and volume of intestinal contents while the aqueous fraction did not have a significant effect. All the fractions produced a significant anti-motility effect either at all doses (chloroform fraction) or at middle and higher doses (methanol and aqueous fractions).

CONCLUSION: The present study demonstrated that the chloroform and methanol fractions possessed significant anti-diarrheal activity. Nevertheless, the aqueous fraction showed only significant anti-motility effect at the higher dose (1000 mg/kg) employed in the study.

Keywords: Anti-enteropooling; Antidiarrheal activity; Castor oil induced diarrhea; Croton macrostachyus; Gastrointestinal transit

References

  1. Prostaglandins. 1976 May;11(5):809-28 - PubMed
  2. J Inflamm (Lond). 2011 Nov 21;8:35 - PubMed
  3. J Ethnobiol Ethnomed. 2009 May 01;5:14 - PubMed
  4. J Pharm Pharmacol. 1993 Feb;45(2):157-9 - PubMed
  5. J Ethnobiol Ethnomed. 2007 Mar 14;3:12 - PubMed
  6. Epilepsy Behav. 2012 Jul;24(3):319-23 - PubMed
  7. Indian J Exp Biol. 2014 Feb;52(2):139-46 - PubMed
  8. BMC Complement Altern Med. 2013 Oct 29;13:291 - PubMed
  9. Prostaglandins Leukot Essent Fatty Acids. 2004 Jun;70(6):511-20 - PubMed
  10. Ann Intern Med. 1985 Feb;102(2):197-9 - PubMed
  11. J Ethnopharmacol. 2000 Apr;70(1):41-55 - PubMed
  12. Gut. 1968 Dec;9(6):655-8 - PubMed
  13. Life Sci. 1997;61(20):2049-55 - PubMed
  14. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9179-84 - PubMed
  15. Phytother Res. 2006 Jan;20(1):82-4 - PubMed
  16. Arch Pharm Res. 2004 Sep;27(9):937-43 - PubMed
  17. BMC Complement Altern Med. 2014 Mar 01;14:79 - PubMed
  18. J Pharmacol Exp Ther. 1994 Jan;268(1):291-5 - PubMed
  19. Evid Based Complement Alternat Med. 2013;2013:170290 - PubMed
  20. Arch Pharm Res. 2004 Mar;27(3):291-4 - PubMed
  21. Biochem Pharmacol. 1999 Sep 1;58(5):759-65 - PubMed
  22. J Pharm Pharmacol. 1978 Jan;30(1):41-5 - PubMed
  23. BMC Public Health. 2012 Mar 21;12:220 - PubMed
  24. Rev Saude Publica. 1995 Dec;29(6):428-33 - PubMed
  25. Lancet. 2013 Apr 20;381(9875):1405-16 - PubMed
  26. Biochem Pharmacol. 1993 Dec 3;46(11):1887-92 - PubMed
  27. Planta Med. 2011 Sep;77(13):1504-11 - PubMed
  28. BMC Complement Altern Med. 2013 May 12;13:101 - PubMed
  29. BMC Complement Altern Med. 2013 Jan 28;13:21 - PubMed
  30. J Pharm Pharm Sci. 2004 Feb 25;7(1):70-5 - PubMed
  31. Planta Med. 1997 Apr;63(2):146-9 - PubMed
  32. J Pharm Pharmacol. 2001 Jun;53(6):867-72 - PubMed
  33. J Ethnobiol Ethnomed. 2009 Oct 12;5:28 - PubMed
  34. J Ethnopharmacol. 2005 Aug 22;100(1-2):37-9 - PubMed
  35. Br J Pharmacol. 1993 Apr;108(4):861-4 - PubMed
  36. Afr J Tradit Complement Altern Med. 2011 Dec 29;9(2):242-9 - PubMed
  37. Phytother Res. 2006 Sep;20(9):717-24 - PubMed
  38. J Clin Invest. 1989 Jun;83(6):1810-20 - PubMed
  39. J Pharm Pharmacol. 1993 Dec;45(12):1054-9 - PubMed

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