Display options
Cite
Kundanati L, Singh SK, Mandal BB, et al. Fabrication and Mechanical Characterization of Hydrogel Infused Network Silk Scaffolds. Int J Mol Sci. 2016;17(10)doi: 10.3390/ijms17101631.
Kundanati, L., Singh, S. K., Mandal, B. B., Murthy, T. G., Gundiah, N., & Pugno, N. M. (2016). Fabrication and Mechanical Characterization of Hydrogel Infused Network Silk Scaffolds. International journal of molecular sciences, 17(10), . https://doi.org/10.3390/ijms17101631
Kundanati, Lakshminath, et al. "Fabrication and Mechanical Characterization of Hydrogel Infused Network Silk Scaffolds." International journal of molecular sciences vol. 17,10 (2016). doi: https://doi.org/10.3390/ijms17101631
Kundanati L, Singh SK, Mandal BB, Murthy TG, Gundiah N, Pugno NM. Fabrication and Mechanical Characterization of Hydrogel Infused Network Silk Scaffolds. Int J Mol Sci. 2016 Sep 26;17(10). doi: 10.3390/ijms17101631. PMID: 27681725; PMCID: PMC5085664.
Copy Download .nbib Format: NLM
Share it on

Int J Mol Sci. 2016 Sep 26;17(10). doi: 10.3390/ijms17101631.

Fabrication and Mechanical Characterization of Hydrogel Infused Network Silk Scaffolds.

International journal of molecular sciences

Lakshminath Kundanati, Saket K Singh, Biman B Mandal, Tejas G Murthy, Namrata Gundiah, Nicola M Pugno

Affiliations

  1. Laboratory of Bio-Inspired & Graphene Nanomechanics, Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano 77, 38123 Trento, Italy. [email protected].
  2. Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati 781039, Assam, India. [email protected].
  3. Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati 781039, Assam, India. [email protected].
  4. Departments of Civil Engineering, Indian Institute of Science, Bangalore 560012, Karnataka, India. [email protected].
  5. Departments of Mechanical Engineering, Indian Institute of Science, Bangalore 560012, Karnataka, India. [email protected].
  6. Laboratory of Bio-Inspired & Graphene Nanomechanics, Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano 77, 38123 Trento, Italy. [email protected].
  7. Center for Materials and Microsystems, Fondazione Bruno Kessler, Via Sommarive 18, Povo, I-38123 Trento, Italy. [email protected].
  8. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK. [email protected].

PMID: 27681725 PMCID: PMC5085664 DOI: 10.3390/ijms17101631

Abstract

Development and characterization of porous scaffolds for tissue engineering and regenerative medicine is of great importance. In recent times, silk scaffolds were developed and successfully tested in tissue engineering and drug release applications. We developed a novel composite scaffold by mechanical infusion of silk hydrogel matrix into a highly porous network silk scaffold. The mechanical behaviour of these scaffolds was thoroughly examined for their possible use in load bearing applications. Firstly, unconfined compression experiments show that the denser composite scaffolds displayed significant enhancement in the elastic modulus as compared to either of the components. This effect was examined and further explained with the help of foam mechanics principles. Secondly, results from confined compression experiments that resemble loading of cartilage in confinement, showed nonlinear material responses for all scaffolds. Finally, the confined creep experiments were performed to calculate the hydraulic permeability of the scaffolds using soil mechanics principles. Our results show that composite scaffolds with some modifications can be a potential candidate for use of cartilage like applications. We hope such approaches help in developing novel scaffolds for tissue engineering by providing an understanding of the mechanics and can further be used to develop graded scaffolds by targeted infusion in specific regions.

Keywords: foam mechanics; mechanical infusion; permeability; silk scaffolds; tissue engineering

Conflict of interest statement

The authors declare no conflict of interest.

References

  1. Biomaterials. 2002 Oct;23(20):4131-41 - PubMed
  2. Philos Trans R Soc Lond B Biol Sci. 2002 Feb 28;357(1418):121-32 - PubMed
  3. Nat Commun. 2015 Apr 28;6:6933 - PubMed
  4. Biorheology. 2004;41(3-4):159-66 - PubMed
  5. J Orthop Res. 2008 Jul;26(7):951-6 - PubMed
  6. Biomaterials. 2007 Dec;28(34):5087-92 - PubMed
  7. J Mech Behav Biomed Mater. 2010 Apr;3(3):278-89 - PubMed
  8. Acta Biomater. 2015 Jan;11:27-36 - PubMed
  9. Biomacromolecules. 2004 May-Jun;5(3):718-26 - PubMed
  10. Eur J Pharm Sci. 2009 May 12;37(2):160-71 - PubMed
  11. J Biomech. 1997 Nov-Dec;30(11-12):1157-64 - PubMed
  12. J Biomech. 2005 Mar;38(3):377-99 - PubMed
  13. Biomaterials. 1992;13(2):67-97 - PubMed
  14. Adv Drug Deliv Rev. 2006 Jun 3;58(3):402-11 - PubMed
  15. Philos Trans A Math Phys Eng Sci. 2006 Jan 15;364(1838):15-30 - PubMed
  16. Macromol Biosci. 2010 Apr 8;10(4):393-403 - PubMed
  17. Nat Protoc. 2011 Sep 22;6(10):1612-31 - PubMed
  18. Biomaterials. 2009 Jan;30(2):196-207 - PubMed
  19. Biotechnol Bioeng. 2009 Jul 1;103(4):655-63 - PubMed
  20. J Mech Behav Biomed Mater. 2013 Dec;28:263-73 - PubMed
  21. Sports Health. 2009 Nov;1(6):461-8 - PubMed

Publication Types