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Trabulo S, Cardoso AL, Mano M, et al. Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems. Pharmaceuticals (Basel). 2010;3(4):961-993doi: 10.3390/ph3040961.
Trabulo, S., Cardoso, A. L., Mano, M., & De Lima, M. C. (2010). Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems. Pharmaceuticals (Basel, Switzerland), 3(4), 961-993. https://doi.org/10.3390/ph3040961
Trabulo, Sara, et al. "Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems." Pharmaceuticals (Basel, Switzerland) vol. 3,4 (2010): 961-993. doi: https://doi.org/10.3390/ph3040961
Trabulo S, Cardoso AL, Mano M, De Lima MC. Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems. Pharmaceuticals (Basel). 2010 Mar 30;3(4):961-993. doi: 10.3390/ph3040961. PMID: 27713284; PMCID: PMC4034016.
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Pharmaceuticals (Basel). 2010 Mar 30;3(4):961-993. doi: 10.3390/ph3040961.

Cell-Penetrating Peptides-Mechanisms of Cellular Uptake and Generation of Delivery Systems.

Pharmaceuticals (Basel, Switzerland)

Sara Trabulo, Ana Luísa Cardoso, Miguel Mano, Maria C Pedroso De Lima

Affiliations

  1. Center for Neuroscience and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Portugal.
  2. Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Apartado 3126, 3001-401 Coimbra, Portugal.
  3. Center for Neuroscience and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Portugal. [email protected].
  4. Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Apartado 3126, 3001-401 Coimbra, Portugal. [email protected].

PMID: 27713284 PMCID: PMC4034016 DOI: 10.3390/ph3040961

Abstract

The successful clinical application of nucleic acid-based therapeutic strategies has been limited by the poor delivery efficiency achieved by existing vectors. The development of alternative delivery systems for improved biological activity is, therefore, mandatory. Since the seminal observations two decades ago that the Tat protein, and derived peptides, can translocate across biological membranes, cell-penetrating peptides (CPPs) have been considered one of the most promising tools to improve non-invasive cellular delivery of therapeutic molecules. Despite extensive research on the use of CPPs for this purpose, the exact mechanisms underlying their cellular uptake and that of peptide conjugates remain controversial. Over the last years, our research group has been focused on the S4

Keywords: cell-penetrating peptides; non-viral vectors; nucleic acid delivery

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