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Della Casa L, Rossi E, Romanelli C, et al. Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes Nutr. 2016;11:26doi: 10.1186/s12263-016-0542-2.
Della Casa, L., Rossi, E., Romanelli, C., Gibellini, L., & Iannone, A. (2016). Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes & nutrition, 1126. https://doi.org/10.1186/s12263-016-0542-2
Della Casa, L, et al. "Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice." Genes & nutrition vol. 11 (2016): 26. doi: https://doi.org/10.1186/s12263-016-0542-2
Della Casa L, Rossi E, Romanelli C, Gibellini L, Iannone A. Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes Nutr. 2016 Oct 04;11:26. doi: 10.1186/s12263-016-0542-2. eCollection 2016. PMID: 27713773; PMCID: PMC5050585.
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Genes Nutr. 2016 Oct 04;11:26. doi: 10.1186/s12263-016-0542-2. eCollection 2016.

Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice.

Genes & nutrition

L Della Casa, E Rossi, C Romanelli, L Gibellini, A Iannone

Affiliations

  1. "ProteoWork Lab", Dipartimento di Medicina Diagnostica, Clinica e di Sanità Pubblica, Università di Modena e Reggio Emilia, via Campi 287, 41125 Modena, Italy.
  2. Dipartimento Chirurgico, Medico, Odontoiatrico e di Scienze Morfologiche con Interesse Trapiantologico, Oncologico e di Medicina Rigenerativa, Università di Modena e Reggio Emilia, via Campi 287, 41125 Modena, Italy.

PMID: 27713773 PMCID: PMC5050585 DOI: 10.1186/s12263-016-0542-2

Abstract

BACKGROUND: The individual genetic variations, as a response to diet, have recently caught the attention of several researchers. In addition, there is also a trend to assume food containing beneficial substances, or to supplement food with specific compounds. Among these, there is the conjugated linoleic acid (CLA), which has been demonstrated to reduce fat mass and to increase lean mass, even though its mechanism of action is still not known. We investigated the effect of CLA isomers (CLA c9,t11 and CLA t10,c12) on the proteomic profile of liver, adipose tissue, and muscle of mouse, with the aim of verifying the presence of a modification in fat and lean mass, and to explore the mechanism of action.

METHODS: C57/BL6 mice were fed for 2 months with different diets: (1) standard chow, (2) CLA c9,t11 diet, (3) CLA t10,c11 diet, (4) CLA isomers mixture diet, and (5) linoleic acid diet. The proteomic profile of liver, white adipose tissue, and muscle was investigated. Statistical significance of the spots with an intensity higher than twofold in expression compared to the control was tested using student's

RESULTS: We found that both isomers modulate the proteomic profiles of liver, adipose tissue, and muscle by different mechanisms of action. Liver steatosis is mostly due to the isomer CLA t10,c12, since it alters the expression of lipogenetic proteins; it acts also reducing the adipose tissue and increasing fatty acid oxidation in muscle. Conversely, CLA c9,t11 has no relevant effects on liver and adipose tissue, but acts mostly on muscle, where it enhances muscular cell differentiation.

CONCLUSIONS: Administration of CLA in humans has to be carefully personalized, since even considering the presence of a species-specific effect, adverse effects might occur on long-term supplementation. Here we demonstrated that, in mouse, CLA is effective in reducing fat mass, but it also induces liver steatosis. The increase of lean mass is linked to an induction of cell proliferation, which, on long-term supplementation, might also lead to adverse effects.

References

  1. Prostaglandins Leukot Essent Fatty Acids. 2015 Jul;98:15-9 - PubMed
  2. J Lipid Res. 2002 Sep;43(9):1400-9 - PubMed
  3. J Biol Chem. 2009 May 22;284(21):14050-7 - PubMed
  4. Behav Brain Res. 2009 Jun 25;200(2):295-303 - PubMed
  5. Annu Rev Nutr. 2001;21:193-230 - PubMed
  6. Lipids. 2016 Feb;51(2):159-78 - PubMed
  7. J Lipid Res. 1999 Aug;40(8):1426-33 - PubMed
  8. Physiol Rev. 2009 Jul;89(3):921-56 - PubMed
  9. J Biol Chem. 1994 Aug 19;269(33):20807-10 - PubMed
  10. J Cell Sci. 2002 Jan 15;115(Pt 2):355-66 - PubMed
  11. J Microbiol Biotechnol. 2011 Dec;21(12):1211-27 - PubMed
  12. Science. 1965 Aug 27;149(3687):981-2 - PubMed
  13. J Biol Chem. 2012 Nov 23;287(48):40732-44 - PubMed
  14. PLoS One. 2012;7(10):e47948 - PubMed
  15. Biochim Biophys Acta. 2004 Jun 1;1682(1-3):120-7 - PubMed
  16. PLoS One. 2012;7(11):e48801 - PubMed
  17. Dev Growth Differ. 2009 Sep;51(7):607-15 - PubMed
  18. Cell. 1983 Mar;32(3):755-61 - PubMed
  19. Proc Nutr Soc. 2004 Feb;63(1):161-6 - PubMed
  20. Mol Cell Biol. 2002 Jul;22(14 ):5114-27 - PubMed
  21. Glycobiology. 2008 Nov;18(11):842-50 - PubMed
  22. Lipids. 2003 Apr;38(4):377-86 - PubMed
  23. Cell Transplant. 2000 Jul-Aug;9(4):519-29 - PubMed
  24. J Nutr. 2005 Jan;135(1):9-13 - PubMed
  25. Obesity (Silver Spring). 2007 Jan;15(1):32-9 - PubMed
  26. Nutr Metab (Lond). 2013 Jan 30;10(1):16 - PubMed
  27. Int J Mol Med. 2007 Sep;20(3):351-8 - PubMed
  28. J Biol Chem. 2009 Apr 24;284(17):11152-9 - PubMed
  29. Prostaglandins Leukot Essent Fatty Acids. 2009 May-Jun;80(5-6):279-87 - PubMed
  30. Biochem J. 1969 Nov;115(3):405-17 - PubMed
  31. Proteomics Clin Appl. 2008 Apr;2(4):478-91 - PubMed
  32. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4320-5 - PubMed
  33. Lipids. 1999 Mar;34(3):235-41 - PubMed

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