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Kamat MM, Vasudevan S, Maalouf SA, et al. Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy. Biol Reprod. 2016;95(3):68doi: 10.1095/biolreprod.116.141069.
Kamat, M. M., Vasudevan, S., Maalouf, S. A., Townson, D. H., Pate, J. L., & Ott, T. L. (2016). Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy. Biology of reproduction, 95(3), 68. https://doi.org/10.1095/biolreprod.116.141069
Kamat, Manasi M, et al. "Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy." Biology of reproduction vol. 95,3 (2016): 68. doi: https://doi.org/10.1095/biolreprod.116.141069
Kamat MM, Vasudevan S, Maalouf SA, Townson DH, Pate JL, Ott TL. Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy. Biol Reprod. 2016 Sep;95(3):68. doi: 10.1095/biolreprod.116.141069. Epub 2016 Aug 10. PMID: 27512154.
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Biol Reprod. 2016 Sep;95(3):68. doi: 10.1095/biolreprod.116.141069. Epub 2016 Aug 10.

Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.

Biology of reproduction

Manasi M Kamat, Sreelakshmi Vasudevan, Samar A Maalouf, David H Townson, Joy L Pate, Troy L Ott

Affiliations

  1. Department of Animal Science, Center for Reproductive Biology and Health, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania.
  2. Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, New Hampshire.
  3. Department of Animal Science, Center for Reproductive Biology and Health, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania [email protected].

PMID: 27512154 DOI: 10.1095/biolreprod.116.141069

Abstract

Establishment of pregnancy requires interaction between the developing conceptus and the uterine mucosal immune system. Myeloid lineage cells (macrophages and dendritic cells) are key mediators of pregnancy in rodents and humans but relatively little is known regarding their role and distribution during early pregnancy in ruminants. We tested the hypothesis that myeloid lineage cell number, distribution, and function are altered during early pregnancy in dairy heifers. Dairy heifers were inseminated using sperm from a single bull (Day 0), and uteri and blood were collected at slaughter on Days 17 and 20 of pregnancy to investigate the response of myeloid lineage cells to the presence of a conceptus. Responses were compared to noninseminated heifers on Day 17 of the estrous cycle. Peripheral blood and uterine-derived immune cells were isolated magnetically and examined using flow cytometry. Immunohistochemical analysis was used to evaluate the spatial distribution of myeloid lineage cells in the endometrium and quantitative polymerase chain reaction was conducted to quantify abundance of mRNA transcripts associated with myeloid lineage cell function. Transcripts for major histocompatibility complex (MHC) II, cluster of differentiation (CD) 80, CD86, CD163, and indoleamine 2,3-dioxygenase (IDO) 1 were greater in endometrium of pregnant compared to cyclic heifers. Immunofluorescence analysis revealed increased labeling for MHCII and SIRPA in pregnant compared to cyclic heifers. There were approximately 50% more CD14

© 2016 by the Society for the Study of Reproduction, Inc.

Keywords: cattle; conceptus; dendritic cell; macrophage; pregnancy; uterus

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