Display options
Cite
Ipsen DH, Tveden-Nyborg P, Rolin B, et al. High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. Nutr Metab (Lond). 2016;13:51doi: 10.1186/s12986-016-0110-1.
Ipsen, D. H., Tveden-Nyborg, P., Rolin, B., Rakipovski, G., Beck, M., Mortensen, L. W., Færk, L., Heegaard, P. M., Møller, P., & Lykkesfeldt, J. (2016). High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. Nutrition & metabolism, 1351. https://doi.org/10.1186/s12986-016-0110-1
Ipsen, David Højland, et al. "High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs." Nutrition & metabolism vol. 13 (2016): 51. doi: https://doi.org/10.1186/s12986-016-0110-1
Ipsen DH, Tveden-Nyborg P, Rolin B, Rakipovski G, Beck M, Mortensen LW, Færk L, Heegaard PM, Møller P, Lykkesfeldt J. High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs. Nutr Metab (Lond). 2016 Aug 09;13:51. doi: 10.1186/s12986-016-0110-1. eCollection 2016. PMID: 27512407; PMCID: PMC4979160.
Copy Download .nbib Format: NLM
Share it on

Nutr Metab (Lond). 2016 Aug 09;13:51. doi: 10.1186/s12986-016-0110-1. eCollection 2016.

High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs.

Nutrition & metabolism

David Højland Ipsen, Pernille Tveden-Nyborg, Bidda Rolin, Günaj Rakipovski, Maria Beck, Line Winther Mortensen, Lasse Færk, Peter Mikael Helweg Heegaard, Peter Møller, Jens Lykkesfeldt

Affiliations

  1. Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, 1870 Frederiksberg C, Denmark.
  2. Diabetes Pharmacology, Global research, Novo Nordisk, Novo Nordisk Park 1, 2760 Måløv, Denmark.
  3. National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark.
  4. Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, 1353 Copenhagen, Denmark.

PMID: 27512407 PMCID: PMC4979160 DOI: 10.1186/s12986-016-0110-1

Abstract

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) and dyslipidemia are closely related. Diet plays an important role in the progression of these diseases, but the role of specific dietary components is not completely understood. Therefore, we investigated the role of dietary sucrose and fat/cholesterol on the development of dyslipidemia and NAFLD.

METHODS: Seventy female guinea pigs were block-randomized (based on weight) into five groups and fed a normal chow diet (control: 4 % fat), a very high-sucrose diet (vHS: 4 % fat, 25 % sucrose), a high-fat diet (HF: 20 % fat, 0.35 % cholesterol), a high-fat/high-sucrose diet (HFHS: 20 % fat, 15 % sucrose, 0.35 % cholesterol) or a high-fat/very high-sucrose diet (HFvHS: 20 % fat, 25 % sucrose, 0.35 % cholesterol) for 16 and 25 weeks.

RESULTS: All three high-fat diets induced dyslipidemia with increased concentrations of plasma cholesterol (p < 0.0001), LDL-C (p < 0.0001) and VLDL-C (p < 0.05) compared to control and vHS. Contrary to this, plasma triglycerides were increased in control and vHS compared to high-fat fed animals (p < 0.01), while circulating levels of free fatty acids were even between groups. Histological evaluation of liver sections revealed non-alcoholic steatohepatitis (NASH) with progressive inflammation and bridging fibrosis in high-fat fed animals. Accordingly, hepatic triglycerides (p < 0.05) and cholesterol (p < 0.0001) was increased alongside elevated levels of alanine and aspartate aminotransferase (p < 0.01) compared to control and vHS.

CONCLUSION: Collectively, our results suggest that intake of fat and cholesterol, but not sucrose, are the main factors driving the development and progression of dyslipidemia and NAFLD/NASH.

Keywords: Cholesterol; Dyslipidemia; Guinea pigs; High-fat diet; Non-alcoholic fatty liver disease; Non-alcoholic steatohepatitis; Sucrose

References

  1. Transl Res. 2016 Feb;168:146-60 - PubMed
  2. Circ Res. 2004 Nov 12;95(10 ):998-1004 - PubMed
  3. Nutr Metab (Lond). 2006 Mar 27;3:17 - PubMed
  4. Comp Med. 2012 Apr;62(2):109-15 - PubMed
  5. Cancer Res. 2002 Aug 1;62(15):4339-45 - PubMed
  6. Int J Obes Relat Metab Disord. 2001 Mar;25(3):434-8 - PubMed
  7. Mutat Res. 2004 Oct 4;554(1-2):279-86 - PubMed
  8. Int J Mol Sci. 2016 Mar 15;17 (3):383 - PubMed
  9. Am J Physiol. 1997 May;272(5 Pt 2):R1571-6 - PubMed
  10. Obesity (Silver Spring). 2016 Mar;24(3):556-67 - PubMed
  11. Pharmazie. 2013 Oct;68(10):850-4 - PubMed
  12. Hepatology. 2002 Apr;35(4):898-904 - PubMed
  13. Comp Med. 2006 Feb;56(1):17-22 - PubMed
  14. Free Radic Res. 2003 Sep;37(9):947-56 - PubMed
  15. J Vis Exp. 2014 Oct 11;(92):e51982 - PubMed
  16. Semin Liver Dis. 2001;21(1):89-104 - PubMed
  17. J Hepatol. 2011 Oct;55(4):941-3 - PubMed
  18. J Gastroenterol Hepatol. 2008 Nov;23(11):1635-48 - PubMed
  19. PLoS One. 2015 Mar 04;10 (3):e0118773 - PubMed
  20. Am J Pathol. 2010 Jul;177(1):153-65 - PubMed
  21. Hepatology. 2005 Jun;41(6):1313-21 - PubMed
  22. Lipids. 1995 Jul;30(7):619-26 - PubMed
  23. Metabolism. 1995 Jul;44(7):855-64 - PubMed
  24. Am J Clin Nutr. 2008 Dec;88(6):1716S-1721S - PubMed
  25. Nutrients. 2013 May 10;5(5):1544-60 - PubMed
  26. Best Pract Res Clin Gastroenterol. 2011 Apr;25(2):319-33 - PubMed
  27. Hepatology. 2003 Apr;37(4):909-16 - PubMed
  28. J Nutr. 2009 Nov;139(11):2067-71 - PubMed
  29. Free Radic Res. 2013 Aug;47(8):602-13 - PubMed
  30. Metabolism. 2011 Feb;60(2):155-64 - PubMed
  31. Cardiovasc Drug Rev. 2004 Spring;22(1):55-70 - PubMed
  32. Nutrients. 2014 Mar 17;6(3):1128-44 - PubMed
  33. Hepatology. 2010 Nov;52(5):1836-46 - PubMed
  34. World J Gastroenterol. 2011 Aug 7;17(29):3377-89 - PubMed
  35. J Appl Physiol (1985). 2007 Jun;102(6):2260-5 - PubMed
  36. Br J Nutr. 2015 May 28;113(10):1539-49 - PubMed
  37. Antioxid Redox Signal. 2011 Sep 1;15(5):1325-65 - PubMed
  38. J Gastroenterol Hepatol. 2011 May;26(5):875-83 - PubMed
  39. Hepatol Res. 2015 Apr;45(4):458-69 - PubMed
  40. Eur J Pharmacol. 2015 Sep 5;762:364-71 - PubMed
  41. Arch Toxicol. 2003 Nov;77(11):651-6 - PubMed
  42. J Hepatol. 2003 Dec;39(6):978-83 - PubMed
  43. Nat Rev Gastroenterol Hepatol. 2011 Jan;8(1):35-44 - PubMed
  44. Oxid Med Cell Longev. 2012;2012:374346 - PubMed
  45. Hepatol Int. 2008 Mar;2(1):39-49 - PubMed
  46. Mutat Res. 2003 Jun 19;527(1-2):91-7 - PubMed
  47. Obesity (Silver Spring). 2009 Oct;17(10):1872-7 - PubMed
  48. Scand J Gastroenterol. 2009;44(4):471-7 - PubMed
  49. Hepatology. 2009 Sep;50(3):772-80 - PubMed
  50. Eur J Nutr. 2010 Feb;49(1):1-9 - PubMed
  51. Br J Nutr. 2014 Dec 14;112(11):1858-72 - PubMed
  52. Metabolism. 1996 Dec;45(12):1527-32 - PubMed
  53. J Nutr. 1996 Oct;126(10):2494-504 - PubMed
  54. Am J Physiol Regul Integr Comp Physiol. 2011 Dec;301(6):R1710-22 - PubMed
  55. Science. 2011 Jun 24;332(6037):1519-23 - PubMed
  56. Int J Food Microbiol. 2010 Jun 15;140(2-3):218-24 - PubMed
  57. Singapore Med J. 2009 Mar;50(3):295-9 - PubMed
  58. J Nutr Biochem. 2014 Feb;25(2):193-200 - PubMed
  59. Nucleic Acids Res. 2002 May 15;30(10):e47 - PubMed

Publication Types