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Hogerheyde TA, Suzuki S, Walshe J, et al. Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem Cells Int. 2016;2016:8310127doi: 10.1155/2016/8310127.
Hogerheyde, T. A., Suzuki, S., Walshe, J., Bray, L. J., Stephenson, S. A., Harkin, D. G., & Richardson, N. A. (2016). Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem cells international, 20168310127. https://doi.org/10.1155/2016/8310127
Hogerheyde, Thomas A, et al. "Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes." Stem cells international vol. 2016 (2016): 8310127. doi: https://doi.org/10.1155/2016/8310127
Hogerheyde TA, Suzuki S, Walshe J, Bray LJ, Stephenson SA, Harkin DG, Richardson NA. Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes. Stem Cells Int. 2016;2016:8310127. doi: 10.1155/2016/8310127. Epub 2016 Aug 28. PMID: 27648078; PMCID: PMC5018328.
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Stem Cells Int. 2016;2016:8310127. doi: 10.1155/2016/8310127. Epub 2016 Aug 28.

Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes.

Stem cells international

Thomas A Hogerheyde, Shuko Suzuki, Jennifer Walshe, Laura J Bray, Sally A Stephenson, Damien G Harkin, Neil A Richardson

Affiliations

  1. School of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
  2. Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.

PMID: 27648078 PMCID: PMC5018328 DOI: 10.1155/2016/8310127

Abstract

Scaffolds prepared from silk fibroin derived from cocoons of the domesticated silkworm moth Bombyx mori have demonstrated potential to support the attachment and growth of human limbal epithelial (HLE) cells in vitro. In this study, we attempted to further optimize protocols to promote the expansion of HLE cells on B. mori silk fibroin- (BMSF-) based scaffolds. BMSF films were initially coated with different extracellular matrix proteins and then analysed for their impact on corneal epithelial cell adhesion, cell morphology, and culture confluency. Results showed that collagen I, collagen III, and collagen IV consistently improved HCE-T cell adherence, promoted an elongated cell morphology, and increased culture confluency. By contrast, ECM coating had no significant effect on the performance of primary HLE cells cultured on BMSF films. In the second part of this study, primary HLE cells were grown on BMSF films in the presence of medium (SHEM) supplemented with keratinocyte growth factor (KGF) and the Rho kinase inhibitor, Y-27632. The results demonstrated that SHEM medium supplemented with KGF and Y-27632 dramatically increased expression of corneal differentiation markers, keratin 3 and keratin 12, whereas expression of the progenitor marker, p63, did not appear to be significantly influenced by the choice of culture medium.

References

  1. Biomaterials. 2011 Jun;32(17):4076-84 - PubMed
  2. Invest Ophthalmol Vis Sci. 2009 Feb;50(2):604-13 - PubMed
  3. Invest Ophthalmol Vis Sci. 2014 Sep 18;55(10):6590-600 - PubMed
  4. J Cell Sci. 2009 Dec 15;122(Pt 24):4473-80 - PubMed
  5. J Cell Biol. 1986 Jul;103(1):49-62 - PubMed
  6. Cornea. 2009 Oct;28(9):1050-4 - PubMed
  7. Surv Ophthalmol. 2007 Sep-Oct;52(5):483-502 - PubMed
  8. Sci Rep. 2015 Jan 22;5:7955 - PubMed
  9. J Funct Biomater. 2015 May 29;6(2):345-66 - PubMed
  10. Methods Mol Biol. 2013;1014:165-78 - PubMed
  11. Arch Ophthalmol. 2006 Dec;124(12):1734-40 - PubMed
  12. Biomaterials. 2012 May;33(13):3529-38 - PubMed
  13. Invest Ophthalmol Vis Sci. 1995 Jul;36(8):1524-9 - PubMed
  14. Ophthalmology. 1995 Oct;102(10):1476-85 - PubMed
  15. J Ocul Pharmacol Ther. 2007 Apr;23(2):172-81 - PubMed
  16. Tissue Eng Part C Methods. 2013 Jul;19(7):531-7 - PubMed
  17. Stem Cells Int. 2016;2016:9798374 - PubMed
  18. Tissue Eng Part A. 2008 Jul;14(7):1203-11 - PubMed
  19. Exp Eye Res. 2001 Aug;73(2):191-202 - PubMed
  20. Biomaterials. 2011 Aug;32(22):5086-91 - PubMed
  21. Curr Eye Res. 1994 Nov;13(11):805-14 - PubMed
  22. Curr Eye Res. 1994 Oct;13(10):765-78 - PubMed
  23. Exp Eye Res. 1999 Apr;68(4):377-97 - PubMed
  24. Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9523-8 - PubMed
  25. Colloids Surf B Biointerfaces. 2015 Dec 1;136:394-401 - PubMed
  26. Curr Eye Res. 1993 Nov;12(11):963-74 - PubMed
  27. Invest Ophthalmol Vis Sci. 1990 Sep;31(9):1903-13 - PubMed
  28. Exp Eye Res. 2006 Feb;82(2):293-9 - PubMed
  29. Biomaterials. 2011 Apr;32(10):2445-58 - PubMed
  30. J Cell Physiol. 1997 Sep;172(3):361-72 - PubMed
  31. Ophthalmology. 1989 May;96(5):709-22; discussion 722-3 - PubMed
  32. Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3301-9 - PubMed
  33. PLoS One. 2015 Dec 16;10(12):e0144571 - PubMed
  34. Toxicol In Vitro. 2011 Sep;25(6):1237-41 - PubMed
  35. J Pharm Pharmacol. 2008 Mar;60(3):299-307 - PubMed
  36. Exp Eye Res. 2013 Feb;107:110-20 - PubMed
  37. Stem Cells Transl Med. 2013 Oct;2(10):758-65 - PubMed
  38. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3156-61 - PubMed
  39. J Tissue Eng Regen Med. 2015 Oct 9;:null - PubMed
  40. Invest Ophthalmol Vis Sci. 2012 Jun 26;53(7):4130-8 - PubMed
  41. Tissue Eng Part A. 2014 Sep;20(17-18):2378-89 - PubMed
  42. Acta Biomater. 2012 Oct;8(10):3732-43 - PubMed
  43. Stem Cell Res Ther. 2014 Apr 28;5(2):60 - PubMed

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