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Ahlen C, Aas M, Krusnell J, et al. A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years. Microb Ecol Health Dis. 2016;27:31148doi: 10.3402/mehd.v27.31148.
Ahlen, C., Aas, M., Krusnell, J., & Iversen, O. J. (2016). A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years. Microbial ecology in health and disease, 2731148. https://doi.org/10.3402/mehd.v27.31148
Ahlen, Catrine, et al. "A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years." Microbial ecology in health and disease vol. 27 (2016): 31148. doi: https://doi.org/10.3402/mehd.v27.31148
Ahlen C, Aas M, Krusnell J, Iversen OJ. A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years. Microb Ecol Health Dis. 2016 Aug 10;27:31148. doi: 10.3402/mehd.v27.31148. eCollection 2016. PMID: 27515183; PMCID: PMC4981653.
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Microb Ecol Health Dis. 2016 Aug 10;27:31148. doi: 10.3402/mehd.v27.31148. eCollection 2016.

A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in 6 years.

Microbial ecology in health and disease

Catrine Ahlen, Marianne Aas, Jadwiga Krusnell, Ole-Jan Iversen

Affiliations

  1. Norwegian Centre for Maritime Medicine, Department of Occupational Medicine, Haukeland University Hospital, Bergen, Norway; [email protected].
  2. Department of Environmental Technology, Microbiology, SINTEF, Trondheim, Norway.
  3. Department of Microbiology, Parasitology and Waterborne Pathogens, Public Health Agency of Sweden, Stockholm, Sweden.
  4. Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway.

PMID: 27515183 PMCID: PMC4981653 DOI: 10.3402/mehd.v27.31148

Abstract

BACKGROUND: Recurrent legionella outbreaks at one and the same location are common. We have identified a single Legionella pneumophila genotype associated with recurrent Legionella outbreaks over 6 years.

METHODS: Field emergency surveys following Legionella outbreaks were performed on a vessel in 2008, 2009 and 2013. Water samples from both the distribution and technical parts of the potable water system were analyzed with respect to L. pneumophila [Real-Time PCR, cultivation, serotyping and genotyping (PFGE)] and free-living amoebae, (FLA).

RESULTS: Legionella pneumophila serogroup 1 was present in the ship's potable water system during every outbreak. Genotyping of the 2008 survey material showed two separate PFGE genotypes while those in 2009 and 2013 demonstrated the presence of only one of the two genotypes. FLA with intracellular L. pneumophila of the same genotype were also detected. Analyses of the freshwater system on a ship following three separate Legionella outbreaks, for L. pneumophila and FLAs, revealed a single L. pneumophila genotype and FLA (Hartmanella).

CONCLUSIONS: It is reasonable to assume that the L. pneumophila genotype detected in the freshwater system was the causal agent in the outbreaks onboard. Persistence of an apparently low-pathogenic L. pneumophila genotype and FLA in a potable water system represent a potential risk for recurrent outbreaks.

Keywords: Free-living amoeba; Genotypes; Legionella pneumophila; Pathogenicity; Prevention and control; Ships; Virulence; infection outbreaks

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