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Woods RJ, Greally JF. A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans. Ir J Med Sci. 1976;145(1):369-77doi: 10.1007/BF02938974.
Woods, R. J., & Greally, J. F. (1976). A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans. Irish journal of medical science, 145(1), 369-77. https://doi.org/10.1007/BF02938974
Woods, R J, and Greally, J F. "A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans." Irish journal of medical science vol. 145,1 (1976): 369-77. doi: https://doi.org/10.1007/BF02938974
Woods RJ, Greally JF. A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans. Ir J Med Sci. 1976 Dec;145(1):369-77. doi: 10.1007/BF02938974. PMID: 27517258.
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Ir J Med Sci. 1976 Dec;145(1):369-77. doi: 10.1007/BF02938974.

A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans.

Irish journal of medical science

R J Woods, J F Greally

Affiliations

  1. Department of Immunology, School of Pathology, Trinity College, Dublin 2.

PMID: 27517258 DOI: 10.1007/BF02938974

Abstract

A micro method forin vitro transformation of lymphocytes (Penhaleet al., 1974) by mitogens and antigens is presented. Using a range of concentrations, satisfactory responses were obtained with small numbers of lymphocytes in the presence of the mitogens, phytohaemagglutinin-P, Concanavalin-A, Pokeweed mitogen and mumps, tetanus, tuberculin (Purified Protein Derivative) antigens. Diptheria toxoid and streptokinase/streptodornase antigens did not give satisfactory responses. The method is likely to be of particular use in studies in lymphopenic individuals.

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