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O'Connell R, Skrabanek P, Cannon D, et al. High-sensitivity radioimmunoassay for substance P. Ir J Med Sci. 1976;145(1):392doi: 10.1007/BF02938978.
O'Connell, R., Skrabanek, P., Cannon, D., & Powell, D. (1976). High-sensitivity radioimmunoassay for substance P. Irish journal of medical science, 145(1), 392. https://doi.org/10.1007/BF02938978
O'Connell, R, et al. "High-sensitivity radioimmunoassay for substance P." Irish journal of medical science vol. 145,1 (1976): 392. doi: https://doi.org/10.1007/BF02938978
O'Connell R, Skrabanek P, Cannon D, Powell D. High-sensitivity radioimmunoassay for substance P. Ir J Med Sci. 1976 Dec;145(1):392. doi: 10.1007/BF02938978. PMID: 27517262.
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Ir J Med Sci. 1976 Dec;145(1):392. doi: 10.1007/BF02938978.

High-sensitivity radioimmunoassay for substance P.

Irish journal of medical science

R O'Connell, P Skrabanek, D Cannon, D Powell

Affiliations

  1. Endocrine Unit, Mater Misericordiae Hospital, Dublin 7.

PMID: 27517262 DOI: 10.1007/BF02938978

Abstract

Optimisation of a non-equilibrium radioimmunoassay for substance P is described. The assay has a sensitivity of 2 pg (1.5 fmole), sufficient for substance P determinations in unexcepted human plasma and other biological material. The specificity of antisera used was tested with closely related peptides, physalaemin and eledoisin. Crossreaction of 1 per cent for eledoisin and 0.1 per cent for physalaemin was found. The assay is easy to perform, has a precision of 5 per cent, and takes only 24 hours.

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