Display options
Cite
Huang CT, Levine MM, Woodward WE, et al. Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity. Lipids. 1981;16(9):670-6doi: 10.1007/BF02535062.
Huang, C. T., Levine, M. M., Woodward, W. E., Gopalakrishna, G. S., Nalin, D. R., Hornick, R. B., & Nichols, B. L. (1981). Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity. Lipids, 16(9), 670-6. https://doi.org/10.1007/BF02535062
Huang, C T, et al. "Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity." Lipids vol. 16,9 (1981): 670-6. doi: https://doi.org/10.1007/BF02535062
Huang CT, Levine MM, Woodward WE, Gopalakrishna GS, Nalin DR, Hornick RB, Nichols BL. Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity. Lipids. 1981 Sep;16(9):670-6. doi: 10.1007/BF02535062. PMID: 27519235.
Copy Download .nbib Format: NLM
Share it on

Lipids. 1981 Sep;16(9):670-6. doi: 10.1007/BF02535062.

Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity.

Lipids

C T Huang, M M Levine, W E Woodward, G S Gopalakrishna, D R Nalin, R B Hornick, B L Nichols

Affiliations

  1. Section of Nutrition and Gastroenterolory, Department of Pediatrics, Baylor College of Medicine, 77030, Houston, TX.
  2. Division of Infectious Diseases, University of Maryland, School of Medicine, 21201, Baltimore, MD.

PMID: 27519235 DOI: 10.1007/BF02535062

Abstract

Fecal steroid compositions of 82 human subjects of various ages and diets and gastrointestinal status were examined by gas liquid chromatography. Progressive increases in bacterial activities on both bile acids and neutral sterols were observed with the advance of age in infants and children. The patterns in the 4-year-olds approached those observed in adults. Bacterial activites on fecal steroids were found to be decreased in adult subjects with acute shigellosis and in those challenged by castor oil. In contrast, no significant changes in fecal steroid profiles were observed in the subjects with traveller's diarrhea assoicated with toxigenicEscherichia coli. The effects of diarrhea on fecal steroids of infants under 1(1/2) years were less consistent than those of adults. However, a close relationship was observed between the degree of 7α-dehydroxylation of cholic acid (expressed as the ratio of deoxycholic to the sum of deoxycholic and cholic acids) and the percentage of cholesterol in the feces (r= 0.921, p<0.001). The correlation between the production of lithocholic acid and the percentage cholesterol was also good (r=-0.739, p<0.001). Analysis of neutral steroids may be a good index of intraluminal bile acid metabolism.

References

  1. Am J Clin Nutr. 1978 Apr;31(4):626-32 - PubMed
  2. Am J Clin Nutr. 1980 Jan;33(1):40-4 - PubMed
  3. J Lipid Res. 1965 Jul;6:411-24 - PubMed
  4. Can J Biochem. 1972 Apr;50(4):402-8 - PubMed
  5. Adv Lipid Res. 1973;11:143-92 - PubMed
  6. Gut. 1973 Oct;14(10):790-3 - PubMed
  7. J Chromatogr. 1974 Dec 4;101(1):235-9 - PubMed
  8. Gastroenterology. 1971 Jul;61(1):85-90 - PubMed
  9. Am J Clin Nutr. 1976 Sep;29(9):949-55 - PubMed
  10. Am J Clin Nutr. 1974 Nov;27(11):1329-40 - PubMed
  11. J Chromatogr. 1975 Jun 18;109(2):427-31 - PubMed
  12. Am J Clin Nutr. 1978 Mar;31(3):516-26 - PubMed
  13. Lipids. 1974 Dec;9(12):1024-9 - PubMed
  14. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):202-10 - PubMed
  15. Am J Clin Nutr. 1970 May;23(5):546-59 - PubMed
  16. Fed Proc. 1963 Nov-Dec;22:1322-9 - PubMed
  17. J Pathol. 1971 Jun;104(2):129-39 - PubMed
  18. Am J Clin Nutr. 1976 Nov;29(11):1196-203 - PubMed
  19. Biochim Biophys Acta. 1968 Jan 10;152(1):165-73 - PubMed

Publication Types