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Lüthy R. Aminoglykosid-Bestimmungen im Serum mit der Urease-Methode. [Assay of aminoglycosides in serum using the urease method]. Infection. 1977;5(2):115-116doi: 10.1007/BF01642092.
Lüthy, R. (1977). Aminoglykosid-Bestimmungen im Serum mit der Urease-Methode. [Assay of aminoglycosides in serum using the urease method]. Infection, 5(2), 115-116. https://doi.org/10.1007/BF01642092
Lüthy, R. "Aminoglykosid-Bestimmungen im Serum mit der Urease-Methode." [Assay of aminoglycosides in serum using the urease method]. Infection vol. 5,2 (1977): 115-116. doi: https://doi.org/10.1007/BF01642092
Lüthy R. Aminoglykosid-Bestimmungen im Serum mit der Urease-Methode. [Assay of aminoglycosides in serum using the urease method]. Infection. 1977 Jun;5(2):115-116. doi: 10.1007/BF01642092. German. PMID: 27722999.
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Infection. 1977 Jun;5(2):115-116. doi: 10.1007/BF01642092.

[Assay of aminoglycosides in serum using the urease method].

Infection

[Article in German]
R Lüthy

Affiliations

  1. Departement für Innere Medizin der Universität Zürich, Medizinische Poliklinik, Rämistr. 100, CH-8091, Zürich, Switzerland.

PMID: 27722999 DOI: 10.1007/BF01642092

Abstract

The urease method developed byNoone et al. for rapid bioassay of aminoglycoside antibiotics in serum is described in detail. The accuracy of the method was improved by using a BM 1-09 electrode assembly (Metrohm, CH-9100 Herisau, Switzerland) in conjunction with a digital pH-Meter (Metrohm E 500). The mean difference between spiked serum samples and measured concentrations was +0.37±0.78µg/ml. The coefficient of determination between the urease and agar diffusion method was 0.94. Disadvantages of the method are a low sensitivity at concentrations below 1.5µg/ml and a large serum sample (1.5 ml). Advantages are its simplicity and rapidity.

References

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