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Fan B, Li YL, Mariappan A, et al. New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42. Biol Open. 2016;5(12):1776-1783doi: 10.1242/bio.021501.
Fan, B., Li, Y. L., Mariappan, A., Becker, A., Wu, X. Q., & Borriss, R. (2016). New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42. Biology open, 5(12), 1776-1783. https://doi.org/10.1242/bio.021501
Fan, Ben, et al. "New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42." Biology open vol. 5,12 (2016): 1776-1783. doi: https://doi.org/10.1242/bio.021501
Fan B, Li YL, Mariappan A, Becker A, Wu XQ, Borriss R. New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42. Biol Open. 2016 Dec 15;5(12):1776-1783. doi: 10.1242/bio.021501. PMID: 27797724; PMCID: PMC5200910.
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Biol Open. 2016 Dec 15;5(12):1776-1783. doi: 10.1242/bio.021501.

New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42.

Biology open

Ben Fan, Yu-Long Li, Aruljothi Mariappan, Anke Becker, Xiao-Qin Wu, Rainer Borriss

Affiliations

  1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
  2. Institut für Biologie/Bakteriengenetik, Humboldt Universität zu Berlin, Chausseestrasse 117, Berlin D-10115, Germany.
  3. LOEWE Center for Synthetic Microbiology, Marburg an der Lahn, Philipps-Universität Marburg, Marburg 35037, Germany.
  4. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China [email protected] [email protected].
  5. Institut für Biologie/Bakteriengenetik, Humboldt Universität zu Berlin, Chausseestrasse 117, Berlin D-10115, Germany [email protected] [email protected].
  6. Fachgebiet Phytomedizin, Albrecht Daniel Thaer Institut für Agrar- und Gartenbauwissenschaften, Lebenswissenschaftliche Fakultät, Humboldt Universität zu Berlin, Berlin 14195, Germany.

PMID: 27797724 PMCID: PMC5200910 DOI: 10.1242/bio.021501

Abstract

The alternative sigma factor D is known to be involved in at least three biological processes in Bacilli: flagellin synthesis, methyl-accepting chemotaxis and autolysin synthesis. Although many Bacillus genes have been identified as SigD regulon, the list may be not be complete. With microarray-based systemic screening, we found a set of genes downregulated in the sigD knockout mutant of the plant growth-promoting rhizobacterium B. amyloliquefaciens subsp. plantarum FZB42. Eight genes (appA, blsA, dhaS, spoVG, yqgA, RBAM_004640, RBAM_018080 and ytk) were further confirmed by quantitative PCR and/or northern blot to be controlled by SigD at the transcriptional level. These genes are hitherto not reported to be controlled by SigD. Among them, four genes are of unknown function and two genes (RBAM_004640 and RBAM_018080), absent in the model strain B. subtilis 168, are unique to B. amyloliquefaciens stains. The eight genes are involved in sporulation, biofilm formation, metabolite transport and several other functions. These findings extend our knowledge of the regulatory network governed by SigD in Bacillus and will further help to decipher the roles of the genes.

© 2016. Published by The Company of Biologists Ltd.

Keywords: Bacillus amyloliquefaciens; FZB42; Microarray; Non-isotopic northern blot; SigD; Sigma factor; Soil extract

Conflict of interest statement

The authors declare no competing or financial interests.

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