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Lesche D, Geyer R, Lienhard D, et al. Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics. 2016;12(10):159doi: 10.1007/s11306-016-1109-3.
Lesche, D., Geyer, R., Lienhard, D., Nakas, C. T., Diserens, G., Vermathen, P., & Leichtle, A. B. (2016). Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics : Official journal of the Metabolomic Society, 12(10), 159. https://doi.org/10.1007/s11306-016-1109-3
Lesche, Dorothea, et al. "Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome." Metabolomics : Official journal of the Metabolomic Society vol. 12,10 (2016): 159. doi: https://doi.org/10.1007/s11306-016-1109-3
Lesche D, Geyer R, Lienhard D, Nakas CT, Diserens G, Vermathen P, Leichtle AB. Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics. 2016;12(10):159. doi: 10.1007/s11306-016-1109-3. Epub 2016 Sep 15. PMID: 27729833; PMCID: PMC5025507.
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Metabolomics. 2016;12(10):159. doi: 10.1007/s11306-016-1109-3. Epub 2016 Sep 15.

Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome.

Metabolomics : Official journal of the Metabolomic Society

Dorothea Lesche, Roland Geyer, Daniel Lienhard, Christos T Nakas, Gaëlle Diserens, Peter Vermathen, Alexander B Leichtle

Affiliations

  1. University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland ; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  2. University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland.
  3. University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland ; Laboratory of Biometry, University of Thessaly, Volos, Greece.
  4. Departments of Clinical Research and Radiology (AMSM), Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland ; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  5. Departments of Clinical Research and Radiology (AMSM), Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.

PMID: 27729833 PMCID: PMC5025507 DOI: 10.1007/s11306-016-1109-3

Abstract

BACKGROUND: Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.

AIM: Determine whether routinely used plasma centrifugation protocols (1500×

METHODS: Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.

RESULTS: Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.

CONCLUSION: Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.

Keywords: Centrifugation; Metabolome; Plasma; Preanalytics; Relative centrifugal force; Spinning time

Conflict of interest statement

All authors declare that they have no conflict of interest directly or indirectly related to the research presented in this manuscript. Informed consent All participants provided written informed cons

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