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Qiu L, Hughes-Brittain NF, Bastiaansen CW, et al. Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films. J Funct Biomater. 2016;7(4)doi: 10.3390/jfb7040033.
Qiu, L., Hughes-Brittain, N. F., Bastiaansen, C. W., Peijs, T., & Wang, W. (2016). Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films. Journal of functional biomaterials, 7(4), . https://doi.org/10.3390/jfb7040033
Qiu, Lin, et al. "Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films." Journal of functional biomaterials vol. 7,4 (2016). doi: https://doi.org/10.3390/jfb7040033
Qiu L, Hughes-Brittain NF, Bastiaansen CW, Peijs T, Wang W. Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films. J Funct Biomater. 2016 Dec 09;7(4). doi: 10.3390/jfb7040033. PMID: 27941669; PMCID: PMC5197992.
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J Funct Biomater. 2016 Dec 09;7(4). doi: 10.3390/jfb7040033.

Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films.

Journal of functional biomaterials

Lin Qiu, Nanayaa F Hughes-Brittain, Cees W M Bastiaansen, Ton Peijs, Wen Wang

Affiliations

  1. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK. [email protected].
  2. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK. [email protected].
  3. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
  4. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK. [email protected].
  5. School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK. [email protected].

PMID: 27941669 PMCID: PMC5197992 DOI: 10.3390/jfb7040033

Abstract

Failures of vascular grafts are normally caused by the lack of a durable and adherent endothelium covering the graft which leads to thrombus and neointima formation. A promising approach to overcome these issues is to create a functional, quiescent monolayer of endothelial cells on the surface of implants. The present study reports for the first time on the use of photoembossing as a technique to create polymer films with different topographical features for improved cell interaction in biomedical applications. For this, a photopolymer is created by mixing poly(methyl methacrylate) (PMMA) and trimethylolpropane ethoxylate triacrylate (TPETA) at a 1:1 ratio. This photopolymer demonstrated an improvement in biocompatibility over PMMA which is already known to be biocompatible and has been extensively used in the biomedical field. Additionally, photoembossed films showed significantly improved cell attachment and proliferation compared to their non-embossed counterparts. Surface texturing consisted of grooves of different pitches (6, 10, and 20 µm) and heights (1 µm and 2.5 µm). The 20 µm pitch photoembossed films significantly accelerated cell migration in a wound-healing assay, while films with a 6 µm pitch inhibited cells from detaching. Additionally, the relief structure obtained by photoembossing also changed the surface wettability of the substrates. Photoembossed PMMA-TPETA systems benefited from this change as it improved their water contact angle to around 70°, making it well suited for cell adhesion.

Keywords: cell interaction; cell proliferation and migration; photoembossing; polymer; surface texture

Conflict of interest statement

The authors declare no conflict of interest.

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