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Garbaj AM, Awad EM, Azwai SM, et al. Enterohemorrhagic . Vet World. 2016;9(11):1184-1189doi: 10.14202/vetworld.2016.1184-1189.
Garbaj, A. M., Awad, E. M., Azwai, S. M., Abolghait, S. K., Naas, H. T., Moawad, A. A., Gammoudi, F. T., Barbieri, I., & Eldaghayes, I. M. (2016). Enterohemorrhagic . Veterinary world, 9(11), 1184-1189. https://doi.org/10.14202/vetworld.2016.1184-1189
Garbaj, Aboubaker M, et al. "Enterohemorrhagic ." Veterinary world vol. 9,11 (2016): 1184-1189. doi: https://doi.org/10.14202/vetworld.2016.1184-1189
Garbaj AM, Awad EM, Azwai SM, Abolghait SK, Naas HT, Moawad AA, Gammoudi FT, Barbieri I, Eldaghayes IM. Enterohemorrhagic . Vet World. 2016 Nov;9(11):1184-1189. doi: 10.14202/vetworld.2016.1184-1189. Epub 2016 Nov 03. PMID: 27956766; PMCID: PMC5146295.
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Vet World. 2016 Nov;9(11):1184-1189. doi: 10.14202/vetworld.2016.1184-1189. Epub 2016 Nov 03.

Enterohemorrhagic .

Veterinary world

Aboubaker M Garbaj, Enas M Awad, Salah M Azwai, Said K Abolghait, Hesham T Naas, Ashraf A Moawad, Fatim T Gammoudi, Ilaria Barbieri, Ibrahim M Eldaghayes

Affiliations

  1. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya.
  2. Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya.
  3. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.
  4. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt.
  5. Department of Genetics, The Lombardy and Emilia Romagna Experimental Zootechnic Institute (IZSLER), Via Bianchi 9, 25124 Brescia, Italy.

PMID: 27956766 PMCID: PMC5146295 DOI: 10.14202/vetworld.2016.1184-1189

Abstract

AIM: The aim of this work was to isolate and molecularly identify enterohemorrhagic

MATERIALS AND METHODS: A total of 108 samples of raw milk (cow, she-camel, and goat) and locally made dairy products (fermented cow's milk, Maasora, Ricotta and ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura and Tobruk) in Libya. Samples were subjected to microbiological analysis for isolation of

RESULTS: Out of 108 samples, only 27 isolates were found to be EHEC O157 based on their cultural characteristics (Tellurite-Cefixime-Sorbitol MacConkey) that include 3 isolates from cow's milk (11%), 3 isolates from she-camel's milk (11%), two isolates from goat's milk (7.4%) and 7 isolates from fermented raw milk samples (26%), isolates from fresh locally made soft cheeses (Maasora and Ricotta) were 9 (33%) and 3 (11%), respectively, while none of the ice cream samples revealed any growth. However, out of these 27 isolates, only 11 were confirmed to be

CONCLUSION: These results can be used for further studies on EHEC O157 as an emerging foodborne pathogen and its role in human infection in Libya.

Keywords: 16S rDNA; dairy products; enterohemorrhagic Escherichia coli O157; milk

References

  1. Vet Ital. 2014 Jul-Sep;50(3):185-91 - PubMed
  2. Libyan J Med. 2014 Jun 11;9:24840 - PubMed
  3. Food Microbiol. 2009 Dec;26(8):865-71 - PubMed
  4. Lancet. 1998 Oct 10;352(9135):1207-12 - PubMed
  5. Int J Food Microbiol. 2001 Jul 20;67(1-2):1-17 - PubMed
  6. Foodborne Pathog Dis. 2009 Sep;6(7):793-806 - PubMed
  7. Front Cell Infect Microbiol. 2012 Jul 12;2:90 - PubMed
  8. Iran J Microbiol. 2013 Sep;5(3):233-8 - PubMed
  9. J Clin Microbiol. 1986 May;23(5):869-72 - PubMed
  10. Appl Environ Microbiol. 1995 Apr;61(4):1669-72 - PubMed
  11. J Food Prot. 2005 Dec;68(12):2623-30 - PubMed
  12. Foodborne Pathog Dis. 2005 Summer;2(2):115-29 - PubMed
  13. Exp Biol Med (Maywood). 2003 Apr;228(4):352-7 - PubMed
  14. Libyan J Med. 2014 Apr 22;9:24409 - PubMed
  15. J Food Prot. 2013 Nov;76(11):1908-15 - PubMed
  16. ISME J. 2011 Oct;5(10):1571-9 - PubMed
  17. ScientificWorldJournal. 2012;2012:231342 - PubMed
  18. J S Afr Vet Assoc. 2008 Dec;79(4):167-70 - PubMed
  19. J Food Prot. 2007 Jul;70(7):1710-6 - PubMed
  20. J Appl Microbiol. 2004;97(4):861-6 - PubMed
  21. Rev Argent Microbiol. 2012 Oct-Dec;44(4):312-23 - PubMed
  22. MMWR Morb Mortal Wkly Rep. 2015 Jun 26;64(24):668-72 - PubMed
  23. Appl Environ Microbiol. 2006 Jan;72(1):144-9 - PubMed
  24. Open Vet J. 2016;6(1):36-43 - PubMed
  25. Food Microbiol. 2009 Dec;26(8):910-4 - PubMed

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